EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.     

中国旱地农业发展的对策

分析了中国旱地基本情况和基本问题,提出了相应对策,介绍了9种旱地农业栽培技术.     

亚麻屑和红麻芯用于仙客来栽培基质的研究初报

为提高亚麻屑和红麻芯的附加值,促进生物资源的综合利用,分别用25%、50%、75%与100%的亚麻屑和红麻芯替代普通泥炭作为仙客来栽培基质.结果表明用25%的亚麻屑或50%的红麻芯替代普通泥炭均有利于仙客来的生长与开花,其各项指标均优于常规基质.     

轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

鸭大肠杆菌病流行病学与病原特性研究

对浙江等地鸭大肠杆菌病流行病学进行调查，并研究其发病特点，分离鉴定出致病性大肠杆菌26株；已对其中20株菌株进行血清型鉴定，主要为078，占70%（14/20），其次是O1，占15%（3/20）；人工感染试验表明，80%（8/10）菌株毒力强，皮下、肌肉、足蹼3种途径均易复制该病；病理组织学检查肝、肺、脾等，表现急性炎症，出血等病变；药敏试验表明，不同菌株对药物敏感性差异较大，除对鸭菌消、卡那霉素等高敏外，对多数常用抗菌药已耐药，最后就该病的防治提出了对策。     

鞭角华扁叶蜂防治研究

对鞭角华扁叶蜂进行5年防治试验,在幼虫危害盛期用1:3000～2000倍20％氰戊菊酯乳油、1:1000倍50％甲胺磷乳油喷雾防治,幼虫越冬期的当年11月～次年2月对受害林地进行深翻,成虫羽化高峰期用1:1500倍50％甲胺磷乳油和80％敌敌畏乳油混合液喷雾防治均能达防治目的。     

crataegus grayana的组织培养

同许多种其它果树一样,山植属的Crataegus grayana也可应用组织培养的方法,进行快速繁殖。在加入0.25ppmBA,0.5ppmIAA以及150ppm水解干酪素的MS培养基上,可由胚得到实生苗。在加入0.5ppm和300ppm水解干酪素的MS培养基上,由茎尖与茎段可得到壮实的试管苗。由于该植物胚在第一年内成熟度甚低,因此用茎尖或茎段进行快繁殖远优于用胚来进行。从此项研究结果看,其繁殖率为2~n-3~n(n为∮月数)。     

研究型大学科研评价指标体系研究

进一步探讨了制定科研评价体系的原则、方法等,并结合目前我国研究型大学的实际情况初步提出了一套科研评价指标体系。     

人工圈养对短尾猴的影响初报

比较安徽黄山鱼鳞坑群短尾猴人工圈养前后的种群生态学和行为学资料表明，人工圈养：（１）降低了个体的迁入率和迁出率，增加了幼体的出生率和幼体死亡率，结果使种群的年平均增长率下降；（２）加速了猴群的分群进程；（３）使个体间的友好行为频率下降，社会联系减弱；同时，攻击行为频率上升，导致群体结构松散。文中分析了人工圈养中人工投饵、人为地限制猴群活动空间范围和游人对上述诸问题的影响机制，并提出了改善和加强管理的措施。     

Influence of ischemia-reperfusion on apoptosis of sinoatrial node cells in rabbits in vivo

AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I_{10 min}, I_{30 min}, I_{60 min} and I_{120 min}) and IR groups (I_{10 min}R_4h, I_{30 min}R_4h, I_{60 min}R_4hand I_{120 min}R_4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I_{10 min} and I_{30 min} groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I_{60 min}, I_{120 min} and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I_{120 min} group and that of Bcl-2 was in I_{60 min} group. ③The highest expression of Fas-L and Bax was observed in I_{60 min}R_4h group. The peak level of Bcl-2 was observed in I_{30 min}R_4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells.