甜菜坏死黄脉病毒(BNYVV)75kDa通读蛋白基因及其54kDa片段的克隆与表达

 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。     

东方杉温室大棚扦插育苗技术

东方杉系杉科属间杂交新树种,半常绿高大乔木,具有耐水湿、耐盐碱、生长速度快、景观效果好、抗风等优良特性;东方杉不结实,且无性繁殖困难。该文介绍了东方杉温室大棚扦插设施准备,采穗圃营建,扦插育苗方法等育苗技术。应用于大规模苗木生产,平均成活率达到76.3%。     

奶牛布鲁氏菌病的病原分离鉴定及血清学研究

通过细菌分离、动物试验、细菌学与血清抗体之间相互关系、温度与感染动物之间相互关系等分离一例奶牛布鲁氏菌。发现细菌学与血清抗体呈平行关系，试管凝集反应与补体结合反应存在一定差异，感染动物14天出现波浪热，持续到剖杀前(31天)。     

大豆室内耐冷筛选及其在田间的应用研究

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。     

山东沂蒙山区主要果树气候区划

应用模糊相似优先比，对沂蒙山区２３个县（市）进行果树气候区划，结果与山东省果树研究所测定的果树品质理化指标相吻合，为沂蒙山区开展以林为主、多种经营，发展名优特产品，加快农民脱贫致富，提供决策依据。     

实验性急性猪丹毒抗原定位和病理学研究

用猪丹毒强毒株人工接种试验猪,采取其心、肺、肾、肝等器官制成样品,光镜和电镜观察结果,靶器官为心、肾、脑、脾、淋巴结,猪丹毒杆菌定位在器官的微血管。     

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

污水污物潜水旋流泵性能及设计方法试验研究

对影响潜水旋流泵性能的6个主要过流件几何参数进行了试验研究，初步掌握了旋流泵叶轮及泵体主要几何尺寸对性能影响的规律。在分析16种优秀水力模型的基础上，计算出叶轮D2及b2函数回归方程式，并用设计实例进行检验，样机效率比国内外同类产品提高3％左右。     

马惠宁输油管道清管的风险分析及对策

介绍了马惠宁输油管道的施工和投产后的运行现状。由于多年运行，管道腐蚀严重，为此拟采用清管器进行内检测。对用清管器进行内检测可能产生的风险进行了分析，并提出了减小风险的对策。     

猪肉毒梭菌毒素中毒的诊断报告

１９９９年７月１５日，遵化市某养猪场的６０ｋｇ左右的育肥猪，因饲喂霉变的小干鱼，引起猪只急性发病和死亡。发病猪只７５头，死亡２１头，治愈５４头，治愈率７２％，现将情况报告如下。１ 临床症状 病猪呆立，食欲废绝，精神沉郁，鼻端、眼结膜发绀，口角流涎并有泡沫，呼吸困难，腹壁松驰，有的腹泻，粪便呈黄绿色或灰绿色水样物，站立不稳或颤抖，尾下垂，不走动，叫声嘶哑，反应迟钝。后肢多呈瘫痪状，卧地不起，最后瞳孔散大，窒息死亡。病猪大多体温正常，个别达４０～４１℃。病程一般在１～３ｄ，个别猪发病到死亡仅 ５～６ ｈ…