TDP-43 mutations in familial and sporadic amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder characterized pathologically by ubiquitinated TAR DNA binding protein (TDP-43) inclusions. The function of TDP-43 in the nervous system is uncertain, and a mechanistic role in neurodegeneration remains speculative. We identified neighboring mutations in a highly conserved region of TARDBP in sporadic and familial ALS cases. TARDBPM337V segregated with disease within one kindred and a genome-wide scan confirmed that linkage was restricted to chromosome 1p36, which contains the TARDBP locus. Mutant forms of TDP-43 fragmented in vitro more readily than wild type and, in vivo, caused neural apoptosis and developmental delay in the chick embryo. Our evidence suggests a pathophysiological link between TDP-43 and ALS.     

Analgesic, anti-inflammatory and antipyretic activities of the ethanolic extract and its fractions of Cleome rutidosperma

Oral administration of the ethanolic extract (200 and 400 mg/kg, p.o) and its fractions (200 mg/kg each) of the aerial parts of Cleome rutidosperma produced significant analgesic activity in acetic acid-induced writhing and tail immersion tests, anti-inflammatory effect against carrageenin induced inflammation and adjuvant induced polyarthritis and antipyretic activity against yeast-induced pyrexia. Fractionation of the ethanolic extract potentiated the activities.     

Pharmacokinetics of lincomycin following single intravenous administration in buffalo calves

Lincomycin 10 mg kg^?1, IV in buffalo calves followed two-compartment open model with high distribution rate constant α (11.2?±?0.42 h^?1) and K ₁₂/K ₂₁ ratio (4.40?±?0.10). Distribution half-life was 0.06?±?0.01 h and AUC was 41.6?±?1.73 μg mL^?1 h. Large Vd_area (1.15?±?0.03 L kg^?1) indicated good distribution of lincomycin in various body fluids and tissues. Peak plasma level of lincomycin (71.8?±?1.83 μg mL^?1) was observed at 1 min as expected by IV route. The elimination half-life and MRT of lincomycin were short (3.30?±?0.08 and 4.32?±?0.11 h, respectively). Lincomycin 10 mg kg^?1 IV at 12-h interval would be sufficient to maintain T?>?MIC above 60 % for bacteria with minimum inhibitory concentrations (MIC) values ≤1.6 μg mL^?1. Favourable pharmacokinetic profile in buffalo calves and a convenient dosing interval suggest that lincomycin may be an appropriate antibacterial in buffalo species for gram-positive and anaerobic bacterial pathogens susceptible to lincomycin.     

Rooting response of Brassica oleraceal. var. capitata and bud formation

Summary Rooting responses of cabbage leaves(Bassica oleracea L. var.capitata) were studied. -indolylbutyric acid (I.B.A.) at a concentration of 2.5 p.p.m. was found suitable for the purpose. Very young and old leaves were found to be unsuitable for successful rooting. In the series A (without axillary bud) the sixth leaf was found to be most suitable, while in the B series (with axillary bud) the fifth as well as the sixth leaves were found to be suitable. These leaves showed the best response by producing longer and maximum number of roots in the shortest time interval. The presence of axillary buds shortened the time period required for the initiation of roots however, no such correlation could be observed in connection with lengthening or larger production of roots. In general, the treated leaves initiated roots earlier than the control. From the successfully rooted leaves with or without axillary buds, secondary buds were produced which grew into fairly big, edible cabbage heads. It is suggested that this method of induced rooting might be fruitful in vegetative propagation of cabbage during the growing season.with 1 plate     

Genotype by Environment (G×E) Interaction Study on Yield Traits in Different Maturity Groups of Rice

Journal of Crop Science and Biotechnology - Rice production is affected by emerging problems of climate change and over-utilization of resources. To obtain consistent yield across diverse...     

Patterns of fish community composition and biodiversity in riverine fish sanctuaries in Bangladesh: Implications for hilsa shad conservation

Understanding biodiversity and community composition is a prerequisite to sustainable exploitation and conservation of fisheries. We assessed the spatial and seasonal patterns of fish community composition and biodiversity in seasonal no-take riverine sanctuaries in Bangladesh, which aim to conserve the anadromous hilsa shad (Tenualosa spp. and Hilsa keele). Fish samples were collected during monsoon, as well as pre- and postmonsoon, from five sites within two sanctuaries and one site in an open fishing area. Although total abundance was similar along the rivers, species richness was significantly higher at the open fishing site due to its connectivity to nearby habitats. The diversity apparently decreased in a downstream direction. Fish communities at two upstream sites were separated from other sites in multivariate space largely due to higher abundance of hilsa at downstream sites and presence of floodplain-oriented species at the two upstream sites. Short body length and high trophic level at all sites indicated that fish communities were overexploited. Our results also indicated that riverine hilsa sanctuaries in Bangladesh are rich in fish species diversity and support several threatened species designated by the International Union for Conservation of Nature (IUCN). Our study also suggested that since hilsa abundance shapes community patterns, it can therefore impact riverine ecosystem functioning in the sanctuary areas. The findings of this study can serve as an important baseline for future biodiversity assessment, conservation and impact assessment of conservation in rivers, and therefore help to establish multispecies ecosystem-based management of riverine fisheries around the world.     

Agroforestry farming systems in the homesteads of Kerala,southern India

Kerala State on the southwestern coast of India in the tropical humid zone has a predominantly agricultural economy, a very high density of population and therefore high pressure on cultivable land. The farmers there undertake cultivation of an array of crops — tree crops, plantation crops, seasonals and biennials — all in intimate mixtures on the same piece of land around the homesteads. Farm animals and poultry and sometimes fisheries also are essential components of the system. The close association of agricultural crops, tree crops and animals in the homesteads represents an excellent example of sustainable and productive agroforestry homegardens. Optimum utilization of available resources of land, solar energy and technological inputs and an efficient recycling of farm wastes are important characteristics of the systems. This paper attempts to describe and evaluate the systems' stability, productivity and sustainability, and identify its merits and constraints as well as research needs.     

Genetic analysis of bacterial wilt resistance in eggplant (Solanum melongena L.)

European Journal of Plant Pathology - Bacterial wilt triggered by Ralstonia solanacearum is one of the major devastating diseases causing significant yield reduction in eggplant. F1 hybrids (18...