Multifunctional colored polyester fabric treated with dopamine hydrochloride at room temperature: higher tensile,hydrophilicity and anti-bacterial properties along with aminolysis

In this work, dopamine hydrochloride, an environmental friendly compound, was applied on polyester fabric through conventional simple impregnation method in alkaline solution (pH=8.5) at room temperature. In situ spontaneous oxidative polymerization of dopamine form polydopamine (PDA) along with aminolysis of polyester fabric surface. Also, a range of colored polyester fabric were successfully achieved by formation of polydopamine adhesive coating layer at different concentration of dopamine hydrochloride (0.001-4 g/l). Fourier transform infrared spectroscopy and field emission scanning electron microscopy showed deposition of polydopmaine on the polyester fabric surface. The modified colored polyester fabric showed reasonable durability against washing, rubbing and light. The treated polyester fabric with 2 g/l dopamine hydrochloride as optimum concentration indicated not only lower spreading time for water droplet and electrical resistance with higher tensile strength but also very good bactericidal activity against Staphylococcus aureus and Escherichia coli.     

Life on the edge - High levels of genetic diversity in a cliff population of Bertya ingramii are attributed to B. rosmarinifolia (Euphorbiaceae)

Wind-facilitated migration of new genotypes into small, geographically disjunct populations should buffer them against local extinction. Bertya ingramii, a monoecious, wind-pollinated shrub, is restricted to three populations in a 4 km² area in eastern Australia. Populations are separated by deeply dissected gorges where it is unlikely that seeds are exchanged but where wind movement may facilitate pollen dispersal. Using 156 highly polymorphic ISSR markers, we found moderate genetic variation within and among populations of B. ingramii and less genetic diversity in a nearby and small population of the widespread Bertya rosmarinifolia. The smallest population of B. ingramii (<30 plants) had the highest genetic variation (65% polymorphic markers, Shannon Information Index = 0.30). AMOVA and a Bayesian analysis showed that molecular variance was equally distributed within and among populations suggesting that gene flow is as limited within as in among populations. Genetic distances between populations were only weakly explained by their relative geographic distances (mantel test, R² = 0.21, P = 0.001) but the distribution of private bands, the departure from Hardy-Weinberg equilibrium, and a UPGMA tree showed that the smallest population of B. ingramii was generally more similar to an upstream population of B. rosmarinifolia. Thirty-eight percent of bands in this small population of B. ingramii were exclusively shared with B. rosmarinifolia. This covert hybridisation may have been an ancient event but may be responsible for contemporary declines in germination and establishment in B. ingramii. The conservation implications are amplified by the endangered status of B. ingramii.     

Evaluation of bone healing in canine tibial defects filled with cortical autograft,commercial-DBM,calf fetal DBM,omentum and omentum-calf fetal DBM

The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 ± 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups.     

Seasonal species diversity and abundance of phytoplankton from the southwestern Caspian Sea

International Aquatic Research - In the present investigation, we aimed to study diversity and cell abundance of phytoplankton in the southwest of the Caspian Sea. This survey included 4 transects...     

Mucoadhesive Chitosan Electrospun Nanofibers Containing Tetracycline and Triamcinolone as a Drug Delivery System

The mucoadhesive Chitosan (CS) nanofibers as a drug delivery system were developed. Chitosan was modified via the immobilization of thiol groups from L-cysteine as a mucoadhesive reagent. The mucoadhesive properties of the chitosan nanofibers were evaluated by tensiometer set and via tensile studies. Drug and mucoadhesive agent loading lead to decrease diameters and increased porous of nanofibers. The release of Tetracycline (Tet) and Triamcinolone (Tri) were increased with increasing immersion time and it became constant at long immersion times. Mucoadhesion studies were done at pH 2–7 and in pH 6 maximum mucoadhesive properties observed. Release studies demonstrated a sustained release of both drug continued up to 48 hours. Microbial studies were performed on the nanofibers. The drug delivery system represented a novel tool for improve the therapeutic efficacy of various drugs that are poorly absorbed from the gastrointestinal tract. Also it is an efficient system for treatment of oral ulceration.     

“Candidatus Phytoplasma solani” associated with Eucalyptus witches’ broom in Iran

During summer of 2015, Eucalyptus camaldulensis plants showing witches’ broom, little leaf and general yellowing of the foliage were observed in west of Fars and Khozestan province of Iran. DNA from samples of 22 symptomatic and two asymptomatic trees was extracted and subjected to molecular analyses. Nested‐PCR test using R16F2n/R16R2 primers confirmed phytoplasma presence in 63% of symptomatic Eucalyptus plants. Sequence analysis along with virtual RFLP of the 16S ribosomal DNA allowed to classify three Eucalyptus witches’ broom strains into the “stolbur” (“Candidatus phytoplasma solani”) 16SrXII‐A subgroup. Comparison of the secA and secY gene sequences with sequences deposited in GenBank confirmed the phytoplasma identity. Real and virtual RFLPs of the amplified secY gene using HaeIII, MseI and RsaI restriction enzymes showed profiles indistinguishable from each other. This is the first study reporting E. camaldulensis as a new host species for “Ca. P. solani.”     

Soil Solution Chemical Response to Two Decades of Experimental Acidification at the Bear Brook Watershed in Maine

We examined long-term changes in soil solution chemistry associated with experimental, whole watershed-acidification at the Bear Brook Watershed in Maine (BBWM). At BBWM, the West Bear (WB) watershed has been treated with bimonthly additions of ((NH₄)₂ SO₄) since 1989. The adjacent East Bear (EB) watershed serves as a biogeochemical reference. Soil solution chemistry in the EB watershed was relatively stable from 1989?C2007, with the exception of declining SO₄?CS concentrations associated with a progressive decline in SO₄?CS deposition during this period. Soil solution chemistry in WB reflected a progressive change in acid-neutralization mechanisms from base cation buffering to Al buffering associated with treatment during this period. Total dissolved Al concentrations progressively increased over time and were ~4× higher in 2007 than in 1989. Treatment of WB was also associated with long-term increases in soil solution H⁺, SO₄?CS, and NO₃?CN, whereas soil solution dissolved organic carbon (DOC) was unresponsive to treatment. For solutes such as Ca, H⁺, and SO₄?CS, changes in stream chemistry were generally parallel to changes in soil solution chemistry, indicating a close coupling of terrestrial and aquatic processes that regulate the chemistry of solutions in this first-order stream watershed. For other solutes such as Al and DOC, solute concentrations were higher in soil solutions compared with streams, suggesting that sorption and transformation processes along hydrologic flow-paths were important in regulating the chemistry of solutions and the transport of these solutes.     

Sol-Gel Synthesis,in vitro Behavior,and Human Bone Marrow-Derived Mesenchymal Stem Cell Differentiation and Proliferation of Bioactive Glass 58S

Background:Bioactive glasses 58S, are silicate-based materials containing calcium and phosphate, which dissolved in body fluid and bond to the bone tissue. This type of bioactive glass is highly biocompatible and has a wide range of clinical applications. Methods:The 58S glass powders were synthesized via sol-gel methods, using tetraethyl orthosilicate, triethyl phosphate, and calcium nitrate, as precursors. Upon the analyses of phase and chemical structures of bioactive glass in different gelation times (12, 48, and 100 h), the appropriate heat treatment (at 525, 575, and 625 °C) was performed to eliminate nitrate compounds and stabilize the glass powder samples. The in vitro assay in SBF solution revealed the bioactivity of the synthesized 58S glass through the morphological (SEM), chemical structure (FTIR), release of calcium, phosphorous and silicon elements, pH variations, and weight loss measurements. The behavior of MSCs in the presence of bioactive glass powders was studied by MTT cytotoxicity, cell staining, ALP activity and biomineralization tests, as well as by the evaluation of ALP, osteocalcin, osteonectin, collagen I, and RUNX2 gene expression. Results: The results confirmed a gelation time of 100 h and a calcination temperature of 575 °C at optimal conditions for the synthesis of nitrate-free bioactive glass powders. Conclusion:The glass spherical nanoparticles in the range of 20-30 nm possess the improved bioactivity and osteogenic properties as demanded for bone tissue engineering. Key Words: Bioactive glass 58S, Gene expression, Mesenchymal stem cells     

Effects of different salinities on amino acid profile in Artemia franciscana

The effects of different salinity levels, including 10–15, 30–35, 70–75, 110–115 and 150–155 ppt, were evaluated on survival and the body amino acids (BAA) of Artemia franciscana. The results were expressed as total essential (TE), non‐essential (NE) and total amino acids (TAA); also, the ratio of the TE to NE (ENAA) on days 3 and 13 of the culture is reported. The study of changes in Artemia BAA showed that with the increase in the water salinity, the TE, NE and TAA increased significantly on days 3 and 13 of the culture. However, the highest ratio of ENAA was observed in the 110 gL^?1 salinity treatment on day 3 (1.067 ± 1.25). Regarding the effects of different salinity treatments on the survival rate of Artemia, it was observed that, generally, an increase in the water salinity would reduce the survival rate of this species. This reduction was observed on day 3 of culture in the salinity treatments above 120 (66.66 ± 1.68%) and below 30 gL^?1 (89.66 ± 0.34%) and on day 13 of culture in the salinity treatments below 30 (11.86 ± 0.13%) and above 70 gL^?1. Basically, it can be concluded that A. franciscana culture at a salinity of 110 gL^?1 can lead to the biological enrichment of Artemia in terms of amino acid profile.     

CONTROLLED RELEASE OF FERTILIZER MICROCAPSULES USING ETHYLENE VINYL ACETATE POLYMER TO ENHANCE MICRONUTRIENT AND WATER USE EFFICIENCY

There have as yet been no detailed studies of micronutrient release from polymer coated fertilizers (PCFs). In this research, slow release microcapsules containing iron fertilizer was made and its release pattern evaluated. Three polymers ethyl cellulose (EC), glycerol mono stearate (GMS) and Compritol 888 ATO, were mixed with avicel and lactose and the microcaopsules were made by extrusion/spheronization method. The dissolution test carried out to determine the Fe release rate versus time. The results showed that, microcaopsules containing Compritol 888 ATO, released iron ions slower than that of EC and GMS. The release rate reached 85% after 7 days. In order to reduce the release rate, these microcaopsules were coated with ethylene vinyl acetate (EVA) polymer by dip coating method. It is concluded that the combination of matrix type formulation and the coating with EVA polymer caused a significant decrease in the release rate of iron.