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1.
Development and characterization of a technique for percutaneous radiologic gastrojejunostomy tube placement in the dog
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Belayneh Ayalew Desalegne Kifle Dagne Gedil Melaku Boukar Ousmane Christian A. Fatokun 《Journal of Crop Science and Biotechnology》2017,20(2):107-128
The extent of genetic diversity and relatedness of cowpea germplasm from East Africa are poorly understood. A set of 13 microsatellites (SSR) and 151 single nucleotide polymorphisms (SNPs) markers were applied to assess the levels of genetic diversity in a sample of 95 accessions of local cowpea germplasm and inbred lines of Vigna unguiculata. The average genetic diversity (D), as quantified by the expected heterozygosity, was higher for SSR loci (0.52) than for SNPs (0.34). The polymorphic information content was 0.48 for SSR and 0.28 for SNP while the fixation index was 0.095 for SSR and 0.15 for SNPs showing moderate differentiation and high gene flow among cowpea accessions from East African countries. The results of data analysis of both SSR and SNP markers showed similar clustering patterns suggesting a substantial degree of association between origin and genotype. Principal coordinate analysis (PCoA) with SSR and SNP markers showed that accessions were grouped into two and three broad groups across the first two axes, respectively. Our study found that SNP markers were more effective than SSR in determining the genetic relationship among East African local cowpea accessions and IITA inbred lines. Based on this analysis, five local cowpea accessions Tvu-13490, Tvu-6378, Tvu-13448, Tvu-16073, and 2305675 were identified to be tightly clustered sharing several common alleles with the drought tolerant variety Danila when analyzed with SSR and SNP markers. The findings will assist and contribute to future genetic diversity studies aimed at the genetic improvement of local Eastern Africa cowpea accessions for improved overall agronomic performance in general and breeding for drought tolerant in particular. 相似文献
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Jeanne D. LestelleDonald L. Thompson Jr. PhD Rebekah C. Hebert MS 《Journal of Equine Veterinary Science》2013
The glucose responses to intravenous injections of a range of doses of recombinant human insulin were determined for six mares known to be insulin sensitive and six mares known to be insulin insensitive, with the goal of better characterizing the regression lines resulting from the two categories of mares. Insulin doses between 8 and 198 mU of insulin per kg of body weight (mU/kg BW) were administered intravenously between September 13 and 26, 2010, starting with 50 mU/kg BW on the first day. Higher and lower doses were administered on alternate days to obtain percentages of decreases in blood glucose concentrations between 10% and 70%. Linear regression analysis revealed that insulin-insensitive mares have glucose response curves with higher y intercepts (P = .066), less steep slopes (P = .0003), and less goodness of fit (P = .053) in addition to the expected greater dose required to produce a 50% reduction in blood glucose concentrations (ED50; P = .006), despite the similarities between their body weights and those of insulin-sensitive mares. Linear and nonlinear regression of responses to the 32, 50, and 79 mU/kg BW insulin doses with the overall estimates of ED50 and the natural log of ED50 indicated that the 50 mU/kg BW dose had the greatest coefficient of determination (>0.95). Generally, it appears that estimates of insulin sensitivity based on a single injection of insulin or on multiple injections of insulin are least variable for insulin-sensitive mares. 相似文献
4.
亚洲地区饲料中霉菌毒素危害的真实情况 总被引:2,自引:0,他引:2
霉菌毒素是动物饲料中具有危害性的成分,它们可导致畜禽多种难以判断的综合病症.霉菌毒素通常导致动物的生产性能下降,免疫机能抑制,导致动物患病,进而通过动物的肉、奶、内脏进入人类的食品链中,危害人类的健康.对这些易受环境和气候影响的天然毒素进行常规地检查和实时监控是非常有必要的.这将有助于饲料生产企业做好控制动物体内霉菌毒素潜在问题的准备,饲料生产企业或通过选择使用正确有效的霉菌毒素吸附剂,添加到饲料中以吸附饲料中的霉菌毒素;或避免购买和使用来自问题地区的霉变饲料原料. 相似文献
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Martin G. Randell Nandhakumar Balakrishnan Rebekah Gunn‐Christie Andrew Mackin Edward B. Breitschwerdt 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(1):45-50
Ineffective erythropoiesis was diagnosed in an 8‐year‐old male castrated Labrador Retriever. Despite treatment with immunosuppressive therapy for suspected immune‐mediated erythrocyte maturation arrest, resolution of the nonregenerative anemia was not achieved. Following documentation of Bartonella henselae bacteremia by Bartonella alpha proteobacteria growth medium (BAPGM) enrichment blood culture, immunosuppressive therapy was discontinued, and the anemia resolved following prolonged antibiotic therapy. Bartonella immunofluorescent antibody testing was negative, whereas B henselae western blot was consistently positive. The contribution of B henselae bacteremia to ineffective erythropoiesis remains unknown; however, the potential role of B henselae in the pathophysiology of bone marrow dyscrasias warrants additional investigation. 相似文献
7.
SB Grant JD Saphores DL Feldman AJ Hamilton TD Fletcher PL Cook M Stewardson BF Sanders LA Levin RF Ambrose A Deletic R Brown SC Jiang D Rosso WJ Cooper I Marusic 《Science (New York, N.Y.)》2012,337(6095):681-686
Humans create vast quantities of wastewater through inefficiencies and poor management of water systems. The wasting of water poses sustainability challenges, depletes energy reserves, and undermines human water security and ecosystem health. Here we review emerging approaches for reusing wastewater and minimizing its generation. These complementary options make the most of scarce freshwater resources, serve the varying water needs of both developed and developing countries, and confer a variety of environmental benefits. Their widespread adoption will require changing how freshwater is sourced, used, managed, and priced. 相似文献
8.
Matthew Stachowiak Shirely E. Clark Rebekah E. Templin Katherine H. Baker 《Water, air, and soil pollution》2010,211(1-4):251-259
We examined the impact of the effluent discharged from a freshwater (trout and related species) fish hatchery on the presence of antibiotic-resistant microorganisms in a small stream. There had been no documented use of antibiotics in the hatchery for at least 6 months prior to our study, although a variety of biocides were employed routinely for cleaning. Heterotrophic bacteria and Escherichia coli were isolated from both water column and sediment samples at sites above and below the discharge of the hatchery effluent as well as from the hatchery effluent itself. Randomly chosen isolates (≥96 isolates per site) were tested for their resistance to ampicillin, cephalexin, erythromycin, and tetracycline. Resistance to at least one antibiotic was found in greater than 30% of both the heterotrophic isolates and the E. coli isolates from each of the sites. There were no significant differences among the sites in the proportion of the heterotrophic isolates resistant to any specific antibiotic. The proportion of E. coli isolates resistant to tetracycline in the hatchery effluent and in both the downstream water and sediment samples was significantly higher than in either the upstream water or sediment. These results support the possibility of the hatchery as a source of tetracycline-resistant microorganisms even in the absence of recent use of this antibiotic. 相似文献
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Chen Hsin-Yi Rawlings Rebekah 《饲料博览》2009,(7):14-17
霉菌毒素是动物饲料中具有危害性的成分,可导致畜禽多种难以判断的综合病征。霉菌毒素通常可导致动物的生产性能下降,免疫机能抑制,并通过动物的肉、奶、内脏进入食品链中,危害人类的健康。对这些易受环境和气候影响的天然毒素进行常规检查和实时监控非常有必要,有助于饲料生产企业做好控制动物体内霉菌毒素潜在问题的准备。 相似文献