1. Dry rabbit excreta were found to contain 188.1 g crude protein, 6.6 g non‐protein nitrogen, 0.3 g ammonia, 89.8 g moisture, 8.0 g ether extractives, 135.2 g crude fibre, 266.9 g ash and 19.18 MJ gross energy/kg. Methionine and lysine contents were 3.95 and 4.29 g/100 g protein, respectively.
2. In one experiment, 0, 50, 100 or 200 g rabbit excreta/kg were substituted for maize in a broiler diet. Chickens receiving the diet containing 200 g rabbit excreta/kg gained significantly less weight to 8 weeks of age than those receiving other diets.
3. In a second experiment, 0, 100, 150 or 200 g rabbit excreta/kg were substituted primarily for maize in a broiler diet to provide equal energy and protein contents. Weight gains to 8 weeks of birds receiving these diets did not differ significantly. The uncorrected metabolisable energy content of the excreta was 9.15 MJ/kg.
4. There were no gross pathological changes in the birds fed on the experimental diets. 相似文献
Our previous study evidenced that the 3D CORAGRAF loaded with PLGA microsphere constitutes PDGF-BB can support cell attachment and proliferation and can induce an osteogenic commitment of mesenchymal stromal cells in the in vitro condition. However, how this construct can perform in pathophysiological conditions in terms of repairing critical bone defects is yet to be understood. A study was therefore conducted to investigate the regeneration potential of calvaria critical-size defects using CORAGRAF + PLGA with PDGF-BB + mesenchymal stromal cells (MSCs) in a rat model. A 5 mm critical bone defect was created on calvaria of 40 male Sprague-Dawley rats. CORAGRAF incorporated either with or without PDGF-BB and seeded with rat bone-marrow-derived MSCs was implanted at the defect region. The bone regeneration potential of implanted constructs was assessed using micro-CT imaging and histological staining in weeks 4 and 8. The micro-CT images indicated a significant closure of defects in the cranial bone of the rats treated with 3D CORAGRAF + PLGA with PDGF-BB + MSCs on week 4 and 8 post-implantation. This finding, further supported with the histology outcome where the rat cranial defect treated with CORAGRAF + PLGA with PDGF-BB + MSCs indicated neo-bony ingrowth with organized and mature bone-like morphology as compared with other groups. The previous in vitro results substantiated with our pre-clinical findings demonstrate that the combination of CORAGRAF + PLGA with PDGF-BB + MSCs could be an ideal construct to support bone regeneration in critical bone defects. Hence, this construct can be further investigated for its safety and efficacy in large animal models, or it can be skipped to human trial prior for commercialization. 相似文献
Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its efficacy.After 10 years of cultivating Bacillus thuringiensis(Bt) cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A) due to its better effectiveness against insect pests.Thus,the current study was designed to evaluate the role of each gene in the effect... 相似文献
Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host-microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty-eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real-time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log10 copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long-term study. 相似文献
The problem of real-time irrigation scheduling under limited water supply is considered. The goal is to develop an irrigation operation policy which maximizes crop yields and is responsive to current season changes in weather and other variables. Because irrigation decisions are sequential and dependent on crop and soil water status, and also because crop yields can only be known at the end of the season, the decisions are arrived at by a two-stage process. In the first or the design stage, irrigations are planned for the entire season at weekly intervals using historical data and an optimal irrigation scheduling model. In the second stage, the decisions for the subsequent weeks are revised each week after updating the status of the system with real time data up to that week and solving the irrigation optimization model once again for the new conditions. Thus, each week an irrigation decision is made, the entire planning horizon is kept in view. The procedure is illustrated by application to a case study. 相似文献