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S. Prosperi A. Irsara G. Battelli V. Sanguinetti 《Veterinary research communications》1984,8(1):181-185
The authors vaccinated 152 cattle divided into three groups against rabies. The first group received the ERA strain and the second group an inactivated vaccine. The third group received the inactivated vaccine on two occasions with an interval of 60 days between the two doses. Their antibody response was surveyed with the fluorescent foci-inhibition test carried out on blood samples collected during a 10-month period. All animals developed an almost identical antibody response. However, at the sixth and tenth months, there was a higher number of seropositive animals in the groups vaccinated with the killed vaccine. 相似文献
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The relationships between welfare and udder health indicators were investigated in 120 midlactation Comisana ewes over a 90-day
period. The welfare indicators monitored were the humoral response to chicken egg albumin (OVA), the cell-mediated immune
response to phytohemagglutinin, and the plasma cortisol levels. Changes in milk components, in somatic cell count (SCC) and
in bacterial count, in leukocyte populations and in anti-OVA IgG concentration were used to monitor the ewe udder health.
Correlations between welfare and udder health indicators were then tested. The skinfold thickness, representative of the cell-mediated
immune response, was correlated positively with the percentage of neutrophils in milk and negatively with the chloride content,
the percentage of lymphocytes and anti-OVA IgG concentrations in milk. The plasma antibody titres to OVA, representative of
the humoral immune response, correlated positively with the anti-OVA IgG levels in milk and negatively with the lactose content
and to the percentage of neutrophils. Plasma cortisol levels correlated positively with the chloride content in milk. Our
study demonstrates that information about the immune condition of animals can be successfully achieved by monitoring the immunological
responses of the mammary gland. The detection of reliable indicators of animal welfare in milk can help in reducing the stress
from animal handling and blood sampling from lactating animals. 相似文献
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Ciulli S. Galletti E. Grodzki M. Alessi A. Battilani M. Prosperi S. 《Veterinary research communications》2007,31(1):221-224
Veterinary Research Communications - Ciulli, S., Galletti, E., Grodzki, M., Alessi, A., Battilani, M. and Prosperi, S., 2007. Isolation and genetic characterization of Betanodavirus from wild... 相似文献
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E. Fernández Valiente A. Quesada C. Prosperi M. Nieva F. Leganés A. Ucha 《Biology and Fertility of Soils》1997,24(4):353-357
Short- and long-term experiments were conducted in the rice fields of Valencia, Spain, to determine the ecological significance
of ammonium on nitrogen fixation. A significant inhibition of nitrogenase activity by ammonium, at concentrations higher than
0.5mM, was observed after 8h of incubation in short-term experiments done with a bloom of the N2-fixing cyanobacterium Anabaena sp. In a second set of short-term experiments for in situ assays of nitrogenase activity in the field, a significant correlation
between nitrogenase activity and the number of N2-fixing cyanobacteria in soil was found. No significant inhibition of nitrogenase activity by ammonium at concentrations up
to 2mM was observed in these assays after 24h of incubation. This lack of inhibition was probably due to the rapid decrease in ammonium
content in the flood water. Only 5% of the ammonium initially added remained in the water 24h later. In the long-term experiments,
nitrogenase activity was assayed in plots fertilized with 0, 70 and 140kgNha–1, over the cultivation cycle, for 5 years. A partial inhibition of nitrogenase activity by deep-placed N fertilizers was observed.
Differences were only significant in 2 years. Mean results from 5 years only showed significant differences between plots
fertilized with 0 and 140kgNha–1. The partial inhibition of nitrogenase activity by ammonium increased over the cultivation cycle. Inhibition was only significant
in September, at the end of the cultivation cycle.
Received: 28 January 1996 相似文献
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Ana C. A. S. Pinheiro Enrico Volpe Donatella Principi Santino Prosperi Sara Ciulli 《Aquaculture International》2016,24(1):115-125
The major viral diseases that affect rainbow trout (Oncorhynchus mykiss) are viral haemorrhagic septicaemia, infectious haematopoietic necrosis, infectious pancreatic necrosis and sleeping disease. In the presented study, we developed a multiplex RT-PCR (mRT-PCR) assay for the simultaneous detection of these four rainbow trout viruses in a single assay. The choice of primers was carried out based on the expected size of the fragments, the temperature and time required for the amplification, and the specificity for the target sequence. Firstly, the method was optimised using reference strains of viral haemorrhagic septicaemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV) and sleeping disease virus (SDV) cultivated with permissive cell culture lines; subsequently, the method was used for the identification of these viral infections in rainbow trout samples. Twenty-two samples of rainbow trout, clinically suspected of having viruses, were analysed by the developed method to detect the presence of the four viruses, by directly analysing the animal tissues. The mRT-PCR method was able to efficiently detect the viral RNA in infected cell culture supernatants and in tissue samples, highlighting the presence of single infections as well as co-infections in rainbow trout samples. VHSV/SDV and IHNV/SDV co-infections were demonstrated for the first time in rainbow trout. The mRT-PCR method was revealed to be an accurate and fast method to support traditional diagnostic techniques in the diagnosis of major viral diseases of rainbow trout. 相似文献