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AIM:To observe changes of serum asymmetry dimethylarginine (ADMA) and endothelin-1 (ET-1) levels,which reflect blood vessel endothelial function,after therapy of enhanced external counterpulsation(EECP) in patients who had taken percutaneous transluminal coronary angioplasty and stent.METHODS:Fifty one coronary heart disease patients (all of them had taken percutaneous tranluminal coronary angioplasty and stent) were distributed into two groups by matching them with ratio of 1∶2,17 patients in EECP group and 34 patients in control group.Both of two groups were given conventional medicine,in addition,EECP group was undertaken three courses of treatment of EECP.ADMA was detected by HPLC-fluorescence method,and ET-1 was detected by radio-immunity method.RESULTS:In EECP group ,compared with prior treatment,ADMA and ET-1 levels was obviously reduced (P<0.05).In control group,compared with prior treatment,ADMA had no significant difference (P>0.05) and ET-1 was higher (P<0.01).Compared between two groups,extent of decrease in ADMA and ET-1 levels in EECP group were more obvious than control group (P<0.01).Improvement of angina and decrease of frequency of angina was more obvious in EECP group than control group.Besides,decrease of ADMA level was positive correlation with them (r=0.85,0.70,respectively P<0.01).CONCLUSION:EECP reduces serum ADMA and ET-1 levels by increasing shear stress to vessel endothelium in coronary heart disease patients who had taken percutaneous tranluminal coronary angioplasty.It hints that EECP can improve endothelial function and provides experimental evidence for the combined treatment of coronary heart disease. 相似文献
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为全面掌握加州新小绥螨四川种群的种群特征,本试验运用两性生命表开展了以朱砂叶螨为猎物的加州新小绥螨四川种群的生长发育、繁殖与捕食率研究。结果表明,加州新小绥螨四川种群不同发育阶段重叠,在雌成螨产卵期结束前雌成螨特定年龄-阶段存活率Sxj明显高于雄成螨。单位时间(12 h)特定年龄-阶段繁殖力fx5、特定年龄繁殖力mx最大值分别为1.74和1.12粒/雌,单雌总产卵量为49.28粒。种群内禀增长率为0.27/d、周限增长率为1.31/d、净增殖率为26.61粒/个体、世代平均周期为12.34 d。在未成熟阶段,加州新小绥螨各螨态特定年龄-阶段捕食率Cxj均呈双峰型,雌成螨和雄成螨捕食率均随时间明显波动。加州新小绥螨取食朱砂叶螨的转化率为4.55、内禀捕食率为0.54、周限捕食率为1.72头。以朱砂叶螨为猎物时,加州新小绥螨四川种群表现出较好的个体发育、种群增长和捕食能力,可作为控制朱砂叶螨的生防资源,值得进一步研究和开发利用;同时朱砂叶螨可作为加州新小绥螨四川种群人工繁殖选择的猎物之一。 相似文献
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台湾桤木采穗圃建立及扦插繁殖技术研究 总被引:1,自引:0,他引:1
经过2a多对台湾桤木采穗圃的建立和管理及扦插试验研究,结果表明:(1)0.5a生优树建立的采穗圃第2a的株采穗量达71条;(2)400mg·L^-1GGR6号生根粉水溶液浸泡的插穗生根率最高,达96.5%,清水处理的穗条效果最差,生根率为67.6%;(3)一年中插穗在6月、9月扦插效果最好,成活率分别可达96.67%、97.35%;(4)插穗摘除1/3叶片扦插效果最好,生根率迭96.3%;(5)母树上部穗条扦插效果最佳,成活率可达97%;(6)台湾桤木插穗以皮部生根为主,属于皮部和愈伤组织混合生根型,夏季扦插60d后成活穗条皮部生根的占83.33%。 相似文献
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Immunogenicity of bovine parainfluenza type 3 virus proteins encapsulated in nanoparticle vaccines,following intranasal administration to mice 总被引:2,自引:0,他引:2
Shephard MJ Todd D Adair BM Po AL Mackie DP Scott EM 《Research in veterinary science》2003,74(2):187-190
Stimulation of long lasting, protective immunity to respiratory viruses is often difficult to achieve with conventional respiratory vaccines. Polymeric nanoparticles, incorporating viral proteins have been shown to offer sustained release of antigen, with consequent prolongued stimulation of the respiratory immune system. In this paper the efficacy of two nanoparticle vaccines (poly-lactide-co-glycolide, PLGA; polymethylmethacrylate, PMMA), incorporating proteins of bovine parainfluenza type 3 virus (BPI-3) was investigated. As a preliminary to experiments in calves, it was considered essential to demonstrate immunogenicity of the experimental vaccine in mice. Mice immunised with PLGA nanoparticles, containing BPI-3 proteins, developed higher levels of virus-specific antibody than mice immunised with the PMMA vaccine or with soluble viral proteins alone. Immunoblotting using serum from the vaccinated mice, demonstrated strong reactions against the major BPI-3 proteins. 相似文献
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Rosochacki SJ Piekarzewska AB Połoszynowicz J Sakowski T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(4):231-242
Forty-five Duroc (recognized as not susceptible to stress) and 34 Pietrain (susceptible to stress) pigs were subjected to immobilization stress in a prone position for 5, 15, 30 and 60 min. Plasma concentrations of epinephrine (E), norepinephrine (NE), dopamine (DA) and cortisol (C) were determined in response to restraint stress. The concentrations of E, NE and DA were different between the two strains of pigs (some significant interactions); the highest response was seen after 5 min of stress. The concentration of plasma C increased with duration of stress and there was a significant interaction between strain of animals and the time of stress. Our data substantiate the use of E, NE, DA and C as indicators of stress in swine as early as 5 min after exposure to the stressor. It is also shown that stress-susceptible Pietrain pigs had higher plasma concentrations of E, NE and DA than Duroc pigs. 相似文献
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杜仲种子发芽试验分析 总被引:3,自引:0,他引:3
杜仲为我国特有的经济树种和名贵中药,其皮为重要的中药材。采用传统的种子育苗法其发芽率一般不超过50%。将杜仲种子剥去果皮或剥去果皮并弃去胚根端部分胚乳,接种于Ms+0.8%琼脂的培养基上进行发芽试验,种子发芽率分别为50%和94%。将种子分别置于10℃、15℃、20℃、25℃、30℃的温度下培养发现,杜仲的最适发芽温度为20℃。 相似文献
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To characterize the structural events associated with incompatibility of graft development, we conducted a histological study of compatible and incompatible pear/pear and pear/quince grafts that had been grown for five months in a greenhouse. Multivariate analysis of histological data describing the structure of the graft union allowed us to discriminate between compatible and incompatible combinations before either macroscopic examination or qualitative microscopic examination differences between graft combinations became evident. The histological variables responsible for the discrimination between incompatible and compatible unions were related to three typical symptoms of graft incompatibility: bark discontinuity, which was the main feature; cambial dysfunction; and accumulation of starch in the scion. Little cell necrosis was observed at the interface of incompatible grafts at the 5-month stage of graft development. Multivariate analysis of histological data provides a new tool for studying early structural events resulting from the graft incompatibility response and for diagnosing early graft incompatibility. 相似文献