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The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen
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The aim of this study was to determine the optimal concentration of curcumin needed for cryopreservation of boar semen. Semen samples (n = 9) were collected from nine Duroc boars which having proven fertility were used for routine artificial insemination. Semen samples were collected and divided into six groups (groups A‐F) according to various concentrations of curcumin in freezing extender (i.e. 0, 0.125, 0.25, 0.50, 0.75 and 1.0 mmol/L, respectively). The semen was frozen by traditional liquid nitrogen vapor method and stored at ?196°C in the liquid nitrogen tank. After storage, frozen semen samples were thawed at 50°C for 12 s and evaluated for progressive motility, viability and acrosome integrity. The present results indicated that the addition of curcumin at 0.25 (group C) or 0.50 mmol/L curcumin (group D) yielded the higher percentage of progressive motility (33.3 and 36.1%, respectively) (P < 0.001). A significantly higher percentage of acrosome integrity was found in groups B (29.7%), C (31.1%) and D (30.2%) than in the other groups (P < 0.01). However, there was no significant difference in percentage of viability among groups. In conclusion, addition to the freezing extender of curcumin during cryopreservation at a concentration of 0.25 or 0.50 mmol/L is the optimal concentration of curcumin for improving the quality (i.e. increased progressive motility and acrosome integrity) of cryopreserved boar semen. 相似文献
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Chanapiwat P Kaeoket K Tummaruk P 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):351-354
The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. 相似文献
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Banjongsinsiri P Shields J Wicker L 《Journal of agricultural and food chemistry》2004,52(26):8214-8223
The effect of vacuum infusion on eggplant quality of a commercial fungal (Aspergillus niger) and citrus pectinmethylesterase (PME) with calcium chloride (4000 ppm) was investigated after processing and during storage. Firmness of infused eggplants using fungal or citrus PME was significantly increased compared to controls (fresh noninfused and water-infused control) after processing and during storage for 7 days at 4 degrees C. Activity of fungal PME-infused eggplant increased almost 32 times, whereas activity of eggplant infused with Marsh grapefruit PME increased 2-fold. Degree of esterification of pectin of eggplants infused with fungal or citrus PME decreased slightly. Cryo-SEM showed that samples treated with fungal PME/ CaCl2 displayed more integrity among cells as compared with water-infused control. The change of pectin in the cell wall was visualized using monoclonal antibodies JIM5 (low-esterified pectin) and JIM7 (high-esterified pectin). JIM5 showed more binding than JIM7 with the cell walls of eggplant tissues from fungal PME/ CaCl2 treatment. 相似文献
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Kaeoket K Chanapiwat P Tummaruk P Techakumphu M Kunavongkrit A 《Tropical animal health and production》2011,43(5):1049-1055
The aim of this study was to evaluate the effect of post-thawing dilution with autologous and heterologous sperm supernatant
on motility of frozen-thawed boar spermatozoa. During the cryopreservation, sperm supernatant (a combination of seminal plasma
and semen extender, 50% v/v) or seminal plasma from nine boars (Duroc, Large White, and Landrace; three in each) was collected by centrifugation and
stored frozen until use as post-thawing solution. Sperm pellets were further processed and cryopreserved using control-rate
freezer and was thawed at 50°C for 12 s. After thawing, frozen thawed semen samples were diluted with seminal plasma (group
A), supernatant from Landrace (group B), supernatant from Large White (group C), supernatant from Duroc (group D), and Modena™
semen extender (group E). Post-thawing motility was evaluated using a phase-contrast microscope after thawing at 1, 10, 20,
and 30 min. The present results show that at 1 min, a significantly higher percentage (P ≤ 0.001) of progressive motility was found in groups B (53.3%) and C (53.9%) than the other groups. At 10 min, the highest
(P ≤ 0.001) progressive motility was found in groups B (65%) and C (61%). At 20 and 30 min, a significantly higher percentage
(P ≤ 0.001) of progressive motility was found in groups B (58.9%), C (53.5%), and D (45.6%) than groups A (3.9%) and E (20.6%).
It can be stated that supernatant from the freezing processes (consisting of seminal plasma and Modena™, 50% v/v) had a beneficial effect on post-thawing progressive motility of frozen boar semen. 相似文献
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Yuri Fukui Moeko Saito Natsuno Nakamura Taichi Mizuno Shuichi Sato Mayu Tsukuda Saori Nakaoka Keita Tsuboi Azusa Sasaki Kouji Kuramochi Panida Boonyaritthongchai Nichapat Kaewmanee Krit Thirapanmethee Mullika Traidej Chomnawang Bhanubong Bongcheewin Thuy Linh Nguyen Huong Lan Thi Nguyen Huong Thi Le Shigehisa Okamoto Takako Nakamura Yasushi Nakamura Nakao Kubo 《Breeding Science》2022,72(2):181
Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on their venation pattern and leaf shape index. We developed 12 SSR markers that allowed good amplification in the Mentha and another Lamiaceae species. In the SSR-based phylogram, the Mentha lines could be delimited into groups I–VI. The Southeast Asian mints divided into groups I and II, and the phylogram separated most of the available species, with groups I and II containing the known species M. × cordifolia and M. arvensis, respectively. The separation of the two groups was supported by a population structure analysis. The SSR markers developed in this study enabled the simultaneous classification of mints and will help improve our understanding of the genetic composition of known mint varieties and as yet unclassified Southeast Asian mints. 相似文献
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