维西傈僳族自治县资源特点及发展建议

维西傈僳族自治县系"三江并流"世界自然遗产的中心腹地,蕴藏着丰富的森林资源、生物资源、旅游资源以及水资源.但由于该县为边疆、民族、山区、贫困县,丰富的自然资源尚未被开发利用,为此提出了保护好现有森林资源、积极发展旅游业、大力开发水电资源、加大生物资源开发利用等发展建议.     

人血小板生成素基因的克隆及CHO稳定细胞系的建立

 以人肝cDNA为模板克隆了人血小板生成素（Human Thrombopoietin,hTPO）基因,利用基因重组技术构建了带有新霉素抗性基因（neo）筛选标记的pcDNA3.1(+)-hTPO真核表达载体,将重组质粒瞬时转染293T细胞,用鼠抗人TPO 单抗Western blot 检测 TPO蛋白的瞬时表达;再将重组质粒转染中国仓鼠卵巢细胞(Chinese Hamster Ovary,CHO),应用400 μg/mL的G418筛选克隆,经PCR及Western blot验证,获得了3株hTPO蛋白表达水平不同的CHO细胞系,为获得大量蛋白并进行活性功能试验及临床应用奠定基础。     

WS镇土地集约利用评价

结合WS镇的土地资源特点和利用现状,建立了一套符合该镇的土地集约利用评价指标体系。采用层次分析法、特尔菲法和综合评价法等方法,对WS镇城镇建设中心区土地集约利用水平进行了评价,并对评价结果进行了分析。     

绿色潮流与北美林产工业

美国林产品杂志58卷第7／8期发表了JimL．Bowyer等的论文,“The Green Movement and the Forest Industry”,较为全面地论述了北美绿色潮流的发展及其对林产工业的影响,预测了未来的发展趋势,对研究林产工业的发展有参考价值。     

葡萄生产主要环节机械化技术探讨

从葡萄生产主要作业环节机械化现状、推广葡萄生产机械化的效益与前景,探讨了葡萄生产机械化急需解决的关键技术。     

毛头鬼伞生物学特性与杀线虫作用的关系

毛头鬼伞Coprinuscomatus,又名鸡腿菇、鸡腿蘑等,隶属真菌门、鬼伞属真菌。该菌肉质肥厚嫩白,鲜美可口。营养丰富,是一种诱人的美味食品。除此之外,该菌还具有独特的医疗保健功效,其味甘性平,有益脾健胃、清神宁智、助消化、增进食欲、治疗痔疮、降低血糖等多种药用功能阻引,是一种极具商业潜能的食药兼用真菌。     

城市湿地公园规划技术研究

介绍城市湿地公园的概念及规划的目的和目标,并研究探讨城市湿地公园规划有关方法和一般技术问题,以提高城市湿地公园的规划与建设水平。     

土壤质地定名法及吸湿水与土壤粒级含量关系的研究

利用卡庆斯基制、美国制、中国制3种土粒分级标准对张家口葡萄产区的30个采样点进行土壤质地命名,并通过对比分析得出中国制是该区最适的土壤质地分类方法。现对吸湿水含量与土壤各粒级微粒百分含量的关系做了探测性研究,得出吸湿水和土壤中小于0.01 mm的粒径含量之间拟合曲线方程为:Y=20.272e0.207x,可根据此方程应用卡庆斯基制确定土壤质地;吸湿水和土壤中1~0.05 mm0、.05~0.002 mm粒径含量的拟合曲线方程分别为:y=22.018x2-105.323x+156.381,y=-18.304x2+86.495x-49.536,又可根据这2个方程利用美国制确定土壤质地名称。这些为用简易方法鉴定土壤质地类型提供科学依据。     

包头市城市环境与经济协调发展分析与评价

运用层次分析法从综合环境效益和综合经济效益两个方面构建指标体系,建立研究模型,定性与定量相结合,从包头市城市环境与经济发展的协调度方面,确定包头市环境与经济协调发展的类型,对2001年-2005年以来包头市的城市环境与经济协调发展状况进行了分析与评价。     

Response of CREG1 promoter to serum starvation in human vascular cells

AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression.