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排序方式: 共有45条查询结果,搜索用时 218 毫秒
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Misa HOSOE Nao YOSHIDA Yutaka HASHIYADA Hidetoshi TERAMOTO Toru TAKAHASHI Sueo NIIMURA 《The Journal of reproduction and development》2014,60(4):268-273
Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in
vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum
replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the
rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%,
0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of
Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes
and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin
and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured
with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater
in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and
blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and
CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in
those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine
oocytes. 相似文献
3.
Yasushi Narita Kei Satoh Keiichi Hayashi Tamami Iwase Shigeru Tanaka Yukiko Dokiya Morikazu Hosoe Kazuhiko Hayashi 《Water, air, and soil pollution》2001,130(1-4):1649-1654
In recent years, acid rain has been a social problem all over the world. In Japan, it is also a big problem especially in the metropolitan area. Then, we have measured major ions such as H+, Na+, NH4 +, K+, Mg2+, Ca2+, Cl?, NO3 ?, and SO4 2? in precipitation and dry deposition samples which had been collected at 9 sampling sites at Hiyoshi, Mita, Kashiwa, Shiki, Fujisawa, Yokosuka, Mitaka, Hachiouji, and Ashikaga in Tokyo Metropolitan area for 10 years since 1990. The average pH of precipitation in their sites was 4.56 (n=1906). As the results of multiple regression analysis showed that pH of precipitation was determined by 5 ions such as NH4 +, nssCa2+(non sea salt calcium), nssCl?(non sea salt chloride), NO3 ?, nssSO4 2?(non sea salt sulfate) in the most of the sampling sites. Therefore, it is very important to investigate the behavior of these ions to understand the acidification of rain in Tokyo Metropolitan area. In this study, a long term trend of each ion concentration in precipitation and wet deposition was also investigated the base on the data we had observed at 7 sites for 10 years by the statistical method. 相似文献
4.
Mishra B Kizaki K Koshi K Ushizawa K Takahashi T Hosoe M Sato T Ito A Hashizume K 《Domestic animal endocrinology》2012,42(2):63-73
Extracellular matrix metalloproteinase inducer (EMMPRIN) and its induced matrix metalloproteinases (MMPs) play a crucial role in tissue remodeling during the peri-implantation period. However, the role of EMMPRIN in the bovine placenta is still unclear. We have postulated that EMMPRIN might play a regulatory role in trophoblastic cell functions during gestation by itself or through the regulation of MMP expression. In this study, EMMPRIN mRNA was detected in the bovine placentome and interplacentome throughout gestation, and its expression was significantly higher in the cotyledon during late gestation. In situ hybridization showed that EMMPRIN mRNA was expressed in the caruncular epithelium and the cotyledonary epithelium, including binucleate cells. Western blot analysis detected a band representing a protein of approximately 65 kDa in the caruncular and cotyledonary tissues, and the intensity of its expression was increased in both of these tissues during late gestation. The expression levels of MMP-2 and MMP-14 in the bovine placenta were higher during late gestation, as was observed for EMMPRIN. Therefore, EMMPRIN might regulate trophoblastic cell functions, especially those of binucleate cells, through MMP expression in the bovine placenta. 相似文献
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Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY) 总被引:1,自引:0,他引:1
Viral diseases constitute a major constraint to high yield and high quality production of potato. Potato leafroll virus (PLRV) and Potato virus Y (PVY) are among the most damaging potato viruses and are prevalent in most potato growing areas. In the present study, attempts
were made to eliminate PLRV and PVY by three cryogenic protocols, i.e., encapsulation-dehydration, encapsulation-vitrification
and droplet. Results showed that both PLRV and PVY could be efficiently eliminated by cryogenic treatments with 83–86% and
91–95% of frequencies of virus-free plantlets obtained for the former and latter, respectively. Frequencies of virus-free
plantlets produced by cryogenic treatments were higher than those by meristem culture (56% for PLRV and 62% for PVY) and thermotherapy
(50% for PLRV and 65% for PVY), and similar to those by thermotherapy followed by meristem culture (90% for PLRV and 93% for
PVY). Survival (75–85%) and regrowth (83–89%) from cryo-treated shoot tips were higher than those from meristem culture (50–55%)
and thermotherapy followed by meristem culture (40–50%), but similar to those from thermotherapy (80–87%). The morphology
of the plantlets regenerated from cryo-treated shoot tips was similar to that of non-treated plantlets. Thus, cryotherapy
would provide an alternative method for efficient elimination of potato viruses, and can be simultaneously used for long-term
storage of potato germplasm and for production of virus-free plants. 相似文献
7.
Amir Hossan Shaikat Shoko Namekawa Sadequllah Ahmadi Misa Takeda Takeshi Ohkubo 《Animal Science Journal》2018,89(4):688-694
The reproductive system in female birds arises as bilateral asymmetrical anlagen, excluding the birds of prey. Earlier, histological and messenger RNA (mRNA) expression profile studies of several genes related to gonadal sex differentiation in chicken embryos tried to elucidate the query of this asymmetry in a scattered manner. To understand the matter precisely, we have focused on mRNA expression of a cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) in second half of the embryonic days (E10–E18). The established role of leptin in development of the embryo and its expression in the embryonic ovary also drove us to check leptin receptor (LEPR) expression in the ovary. Increased expression of FSHR and CYP19A1 in the left ovary compared with that in the right ovary was identified (P < 0.05), promoting preferential left ovarian development and functionality. Significant high expression (P < 0.05) of the apoptotic genes in the right ovary were also involved here. Leptin probably has no direct influence on ovarian asymmetry as no significant variation in gonadal mRNA expression of LEPR was observed within the same experimental days. We propose that asymmetric expression of this cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) leads to the development of dimorphic gonads during embryogenesis. 相似文献
8.
Takashi?Nakatsuka Misa?Saito Yuka?Sato-Ushiku Eri?Yamada Takashi?Nakasato Nobue?Hoshi Kazumichi?Fujiwara Takashi?Hikage Masahiro?NishiharaEmail author 《Euphytica》2012,184(3):335-344
We developed molecular markers for discrimination of white and blue flower color in Japanese gentian plants. White-flowered
gentians can be classified into two types, based on genetic and physiological features. One type includes four allelic variations
(gtmyb3-1, gtmyb3-2, gtmyb3-3, and gtmyb3-4) of an anthocyanin biosynthetic regulator gene (GtMYB3), distinguished by three PCR-based molecular markers. The other type contains a newly identified inactive allele (ans1) of the anthocyanidin synthase (ANS) gene with a premature stop codon generated from a 4-bp deletion in the second exon. The ans1 allele was distinguished from the active ANS allele by a cleaved amplified polymorphism sequence (CAPS) marker. The genotypes of 12 white-flowered gentian cultivars/lines
could be identified and classified as either ans1 or gtmyb3 using these four molecular markers. No white-flowered gentians contained ans1 and gtmyb3 alleles simultaneously. The mutated ANS gene co-segregated with white flower color in an F2 population, demonstrating that the CAPS marker is useful to discriminate between white and blue flowers in gentian. Markers
to discriminate flower color in Japanese gentian will be useful for early selection of progeny and for breeding management. 相似文献
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