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1.
Funaba M Yamate T Narukawa Y Gotoh K Iriki T Hatano Y Abe M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):337-339
Feeding dry foods supplemented with urine acidifier (D,L-methionine (Met) or ammonium chloride) decreased urinary pH and struvite activity product in clinically normal cats. As a result, the number of struvite crystals in urine was greatly reduced. Supplementation with 3% Met but not 1% Met caused decrease in the urinary concentration of sediment, which resulted from a reduction in the HCl-soluble fraction. The concentration of HCl-insoluble sediment was not affected by supplementation with the urine acidifier. 相似文献
2.
Sakae ARASE Chang-Min ZHAO Kazuya AKIMITSU Mikihiro YAMAMOTO Masahiko ICHII 《Journal of General Plant Pathology》2000,66(2):109-116
A gamma-ray-induced rice mutant, M1009, which spontaneously forms brown specks on leaves was isolated. In appearance, these
lesions very much resemble those that are produced during the hypersensitive resistance response of rice to pathogens. A similar
phenotype has been associated with a number of mutants in maize or Arabidopsis, which have been briefly categorized as disease lesion mimics. Brown speck formation was suppressed at temperatures of 25°C
and above, and was severe at 20°C. Light irradiation is also required to initiate brown specks. In addition, the mutant exhibits
heightened resistance to two major fungal pathogens of rice, Magnaporthe grisea and Cochliobolus miyabeanus. Genetic characterization of the mutant has indicated that its les-bs (lesion-brown speck) phenotype segregates as a monogenic
recessive mutation.
Received 13 September 1999/ Accepted in revised form 15 December 1999 相似文献
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Takuma Sugimoto Shinya Yoshida Masataka Aino Kazuhiko Watanabe Kuniko Shiwaku Mikihiro Sugimoto 《Journal of General Plant Pathology》2006,72(2):92-97
Since 1987, Phytophthora root and stem rot of soybean [Glycine max (L.) Merr. cv. Tanbakuro], caused by Phytophthora sojae Kaufman and Gerdemann, has been increasing in the Sasayama, Nishiwaki, and Kasai regions in Hyogo, the most famous soybean
(cv. Tanbakuro)-producing areas in Japan. In 2002 to 2004, 51 isolates (one from each field) of P. sojae were recovered from 51 fields in Hyogo. These isolates were tested for virulence on six Japanese differential soybean cultivars
used for race determination in Japan, and three additional ones containing four Rps genes used in Indiana, USA. Race E was the most prevalent from 2002 to 2004, followed by races A, C, D, and four new races
(proposed as races K, L, M, and N). Interestingly, none of the new races had high virulence on the Japanese differential cultivars,
compared with other races in each area. One (race N) was avirulent on all six soybean differentials. There was a difference
in race distribution on each of three individual areas; race E seemed to be a major component of the P. sojae population in Sasayama, whereas race A and the new race M were the most prevalent in Nishiwaki and Kasai, respectively. Rps6 (cv. Altona) and Rps1a + Rps7 (cv. Harosoy 63) were infected by 90.2% and 33.3% of all isolates, respectively. However, Rps1d (cv. PI103091) was not susceptible to any of the 51 isolates, nor was cv. Gedenshirazu-1. These two soybean cultivars were
considered to be potential sources of resistance to breed new resistant cultivars with the desirable characteristics of cv.
Tanbakuro for this region. 相似文献
5.
Kazuhiro TAMURA Mikihiro YOSHIE Keisuke HASHIMOTO Eiichi TACHIKAWA 《The Journal of reproduction and development》2014,60(6):447-453
Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E2 secretion. These findings suggest the possibility that
luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL. 相似文献
6.
A monitoring test for arsenic species in white rice flour was developed and applied to flours made from 20 samples of polished rice collected from locations all over Japan. The arsenic species in white rice flour made from five samples each of four types of rice were analyzed by HPLC-ICP-MS after a heat-assisted aqueous extraction. The total arsenic and major and minor element concentrations in the white rice flours were measured by ICP-MS and ICP-OES after microwave-assisted digestion. 91 ± 1% of the arsenic in the flours was extractable. Concentrations of arsenite [As(III)], arsenate [As(V)], and dimethylarsinic acid (DMAA) were closely positively correlated with the total arsenic concentrations. The total arsenic concentration in flours made from rice collected around Japan was 0.15 ± 0.07 mg kg(-1) (highest, 0.32 mg kg(-1)), which is very low. It was thus confirmed that the white rice flour samples collected in this experiment were not suffered from noticeable As contamination. 相似文献
7.
Yasunori Akagi Masatoki Taga Mikihiro Yamamoto Takashi Tsuge Yukitaka Fukumasa-Nakai Hiroshi Otani Motoichiro Kodama 《Journal of General Plant Pathology》2009,75(2):101-109
To analyze the genetics of host-specific toxin production and its relation to the specific pathogenicity of a mitosporic fungus
Alternaria alternata, we developed a protoplast fusion system. Protoplasts of drug-resistant transformants of the A. alternata tomato pathotype (AAL-toxin producer) and A. alternata strawberry pathotype (AF-toxin producer) were fused by electrofusion. Of five fusion strains examined, two strains were pathogenic
on both tomato and strawberry host plants, whereas the rest of the fusion strains were pathogenic only on tomato. Pulsed-field
gel electrophoresis analysis demonstrated that the hybrid strains pathogenic on both tomato and strawberry carry 1.0- and
1.05-Mb conditionally dispensable (CD) chromosomes derived, respectively, from the parental strains of the tomato and strawberry
pathotypes. On the other hand, the fusion strains appeared to maintain only a single homologous chromosome derived from one
of the parental strain in the case of essential chromosomes (A chromosomes). The results suggest that fusion strains between
two different pathotypes of A. alternata might be haploid resulting from the deletion of extra sets of essential chromosomes in the fused nuclei, whereas the CD chromosomes
derived from each parental strain could be maintained stably in a new genetic background with an expanded range of pathogenicity.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank database under the accession numbers AB469331to
AB469354. 相似文献
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Hiroyuki?Takahara Gento?Tsuji Yasuyuki?Kubo Mikihiro?Yamamoto Kazuhiro?Toyoda Yoshishige?Inagaki Yuki?Ichinose Tomonori?ShiraishiEmail author 《Journal of General Plant Pathology》2004,70(2):93-96
We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes. 相似文献
10.
Yoshiaki Harimoto Takayoshi Tanaka Motoichiro Kodama Mikihiro Yamamoto Hiroshi Otani Takashi Tsuge 《Journal of General Plant Pathology》2008,74(3):222-229
The apple pathotype of Alternaria alternata produces the cyclic depsipeptide AM-toxin and causes Alternaria blotch of apple. Previously, we cloned AMT2 from the apple pathotype as an orthologue of AFTS1, which is required for biosynthesis of the decatrienoic acid ester AF-toxin I of the strawberry pathotype. These genes were
predicted to encode aldo-keto reductases involved in biosynthesis of a common precursor, 2-hydroxy-isovaleric acid, of AF-toxin
I and AM-toxin. In this study, we analyzed the function of AMT2 in AM-toxin biosynthesis in the apple pathotype. DNA gel blot analysis of the apple pathotype strain IFO8984 with five restriction
enzymes suggested that this strain has a single copy of AMT2 in the genome. However, gene disruption experiments showed that IFO8984 probably has three copies of AMT2. We made mutants having one or two copies of AMT2 disrupted. The single-copy mutants produced less AM-toxin than did the wild type and were still as pathogenic as the wild
type. The two-copy mutants produced trace or undetectable amounts of AM-toxin and were markedly reduced in pathogenicity.
Thus, AMT2 was verified to be required for AM-toxin biosynthesis and hence pathogenicity. The fact that the two-copy mutants have a
remaining copy of AMT2 suggests that multiple copies of AMT2 are prerequisite for the pathogen to produce enough AM-toxin for full pathogenicity. 相似文献