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排序方式: 共有260条查询结果,搜索用时 156 毫秒
1.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
2.
Kumagai R Nakatani K Ikeya N Kito Y Kaidoh T Takeuchi S 《Veterinary microbiology》2007,122(1-2):190-195
In 13 of 43 non-beta-hemolysin-producing bovine isolates of Staphylococcus aureus, two truncated beta-hemolysin (hlb) genes were demonstrated by PCR and sequencing, and one truncated hlb gene was located beside the integrase (int) gene of phage origin. The staphylokinase (sak) gene was detected in all 13 isolates in which the truncated hlb genes were detected by PCR. Enterotoxin A (sea) and enterotoxin P (sep) genes were also detected in 5 and 2 of the 13 isolates, respectively. Moreover, the scn and chp genes encoding staphylococcal complement inhibitor (SCIN) and chemotaxis inhibitory protein of S. aureus (CHIPS) were detected in 13 and 4 of the 13 isolates, respectively. The bacteriophage induced by mitomycin C treatment was able to lysogenize one beta-hemolysin-producing isolate of S. aureus, and the sak and scn genes were detected from the lysogenized isolate. These results suggest quadruple or quintuple conversion of hlb, sak, sea (or sep), scn, and chp genes by bacteriophages among non-beta-hemolysin-producing bovine isolates of S. aureus. 相似文献
3.
Hiroharu Murakami Seiya Tsushima Takayuki Akimoto Yukiko Kuroyanagi Yoshihiro Shishido 《Soil Science and Plant Nutrition》2013,59(8):1307-1311
The effect of the plowing of clubbed roots of cracifers on the population of Plasmodiophora brassicae in soil was quantitatively studied by measuring the number of resting spores produced in the diseased plants. Though the mean number of resting spores per diseased plant increased with the increase of the disease severity, it remained almost identical for the disease severity classified into category 3 among host species and cultivars tested. Mean number of resting spores per diseased plant ranged from 9.3 to 10.9 (log) regardless of the value of the disease index. When the number of resting spores in soil was calculated based on these data and plant cultivation methods, the values were equivalent to 4.8-6.4 resting spores g-1soil (log)where clubroot disease occurred severely. The value of the disease index of Chinese cabbage plants grown in the pots where clubbed roots of initially grown plants had been plowed into soil (plowing plot) was higher than that in the pots where no plants had been grown (control plot) and where the clubbed roots of initial plants had been removed (removal plot). Though the number of resting spores of P. brassicae in soil decreased by 14% of the inocoKum concentration immediately after the inoculation, the number of spores after the first cultivation in the removal plot was similar to that in the control plot. On the other hand, the number of resting spores in the plowing plot increased significantly compared with that in the control plot. The plowing of clubbed roots into soil resulted in the increase of the population of P. brassicae and disease severity of clubroot in subsequent cultivation in the field. The results corresponded to the values estimated based on the number of resting spores in soil in relation to each value of the disease index. 相似文献
4.
Olivier M Aggarwal A Allen J Almendras AA Bajorek ES Beasley EM Brady SD Bushard JM Bustos VI Chu A Chung TR De Witte A Denys ME Dominguez R Fang NY Foster BD Freudenberg RW Hadley D Hamilton LR Jeffrey TJ Kelly L Lazzeroni L Levy MR Lewis SC Liu X Lopez FJ Louie B Marquis JP Martinez RA Matsuura MK Misherghi NS Norton JA Olshen A Perkins SM Perou AJ Piercy C Piercy M Qin F Reif T Sheppard K Shokoohi V Smick GA Sun WL Stewart EA Fernando J Tejeda Tran NM Trejo T Vo NT Yan SC Zierten DL Zhao S 《Science (New York, N.Y.)》2001,291(5507):1298-1302
We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases. 相似文献
5.
Nahoko Kurachi Tomiyasu Miyaura Jumpei Toriyama Yojiro Matsuura Akira Osawa 《Scandinavian Journal of Forest Research》2019,34(2):102-114
We estimated the aboveground net primary production (ANPP) in five self-thinning jack pine (Pinus banksiana) stands in Wood Buffalo National Park, NWT, Canada. The stands (11 to ca.175 years old) were selected to examine the relationship between stand density and tree size and its effect on carbon dynamics. Aboveground litterfall was collected from each stand from 1997 to 2012. Stand biomass was estimated by measuring tree size every 5 years and estimating the individual mass using allometric relationships. ANPP was then estimated by summing the litterfall mass, dead stem mass increment and stand biomass increment. We determined the proportional contribution of each organ to the total litter and the seasonal pattern of needle litterfall. There was a lower turnover rate of aboveground biomass in older stands than younger stands. The ANPP increased in the youngest stand (<30 years old) showed a decreasing trend in stands >50 years old. The maximum ANPP was estimated to be ca. 500?g m?2 year?1 in dry matter, which was found in 30–50 year-old stands. 相似文献
6.
Journal of Plant Diseases and Protection - Three isolates of chrysanthemum white rust fungus, Puccinia horiana, were collected from two regions in Hiroshima Prefecture, Japan, in 2017. In an in... 相似文献
7.
S. Hashimoto S. Ugawa K. Morisada M. Wattenbach P. Smith Y. Matsuura 《Soil Use and Management》2012,28(1):45-53
Forest management and climate change may have a substantial impact on future soil organic carbon (SOC) stocks at the country scale. Potential SOC in Japanese forest soils was regionally estimated under nine forest managements and a climate change scenario using the CENTURY ecosystem model. Three rotations (30, 50, 100 yr) and three thinning regimes were tested: no‐thinning; 30% of the trees cut in the middle of the rotation (e.g. 15 year in a 30‐yr rotation) and thinned trees all left as litter or slash (ThinLef) and the trees from thinning removed from the forest (ThinRem). A climate change scenario was tested (ca. 3 °C increase in air temperature and 9% increase in precipitation). The model was run at 1 km resolution using climate, vegetation and soil databases. The estimated SOC stock ranged from 1600 to 1830 TgC (from 6800 to 7800 gC/m2), and the SOC stock was largest with the longest rotation and was largest under ThinLef with all three rotations. Despite an increase in net primary production, the SOC stock decreased by 5% under the climate change scenario. 相似文献
8.
9.
Sato Y Matsuura S Kadota K Miyazawa I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(1):49-52
Lymphoma was seen in an 11-year-old female savanna monkey (Ceropithecus aethiops). The superficial inguinal and visceral lymph nodes were markedly enlarged, and their architecture was completely effaced by neoplastic cells. The neoplastic cells, which were highly pleomorphic, resembled those in adult T-cell lymphoma-leukemia in humans. Ultrastructurally the neoplastic cells were characterized by nuclear irregularity and clustered dense bodies, and almost all cells showed positivity for CD3. The animal had been reared with her family, and her mother and 2 brothers had antibodies reactive to human T-cell leukemia virus. This virus serologically cross-reacts with simian T-cell leukemia virus, which may be the causative agent of the present neoplasm. 相似文献
10.