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排序方式: 共有230条查询结果,搜索用时 15 毫秒
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Kentaro OKUNO Mitsuro KAMEYA-IWAKI Minoru TAKESHITA Naruto FURUYA Yoichi TAKANAMI 《Journal of General Plant Pathology》2002,68(1):108-109
A Cucumber mosaic virus was newly isolated from Silene armeria and was characterized by biological, serological and molecular biological methods.
Received 4 July 2001/ Accepted in revised form 28 August 2001 相似文献
4.
Ohashi K Sato Y Iwata H Kawai M Kurebayashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1223-1228
Colonic mucosal mast cells are implicated in the pathogenesis of visceral hypersensitivity associated with irritable bowel syndromes. This study was designed to investigate the roles of mucosal mast cells in development of an experimental visceral hypersensitivity induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in rats. TNBS, when injected into the proximal colon through laparotomy, produced a significant decrease in pain threshold of the distal colon to mechanical distention, indicating a visceral hypersensitivity. In the proximal colon that was directly insulted by TNBS, mucosal necrosis and extensive inflammatory cell infiltration were observed with concomitant increase in tissue myeloperoxide (MPO) activity. In the distal colon where distention stimuli were applied, the number of mucosal mast cells significantly increased following TNBS treatment, although neither mucosal injury nor increase in tissue MPO activity was observed. In an organ culture, spontaneous release of a mucosal mast cell-specific protease (RMCP-2) from the distal colon tissue of TNBS-treated rats was significantly larger than that of sham animals. Furthermore, TNBS-induced visceral hypersensitivity was significantly suppressed by subcutaneous pretreatment with a mast cell stabilizer doxantrazole in a dose-dependent manner. These findings suggest that prominent colonic mast cell infiltration associated with an enhanced spontaneous mediator release is responsible, at least partly, for development of visceral hypersensitivity induced by TNBS in rats. 相似文献
5.
Kyoko Suzuki Tomoyasu Yoshitomi Yoichi Kawaguchi Masaki Ichimura Kaneaki Edo Tsuguo Otake 《Fisheries Science》2011,77(3):313-320
Sakhalin taimen Hucho perryi populations have decreased drastically to near extinction. It is urgent to establish an effective conservation strategy based
on an understanding of the characteristics of migration and habitat use of this species. We examined the migration history
of anadromous Sakhalin taimen captured off the Sarufutsu coast, northern Hokkaido, Japan, using otolith Sr:Ca ratios and also
examined the relationship between their otolith Sr:Ca ratios during freshwater and seawater residence in a rearing experiment.
Otolith Sr:Ca ratios of some fish from the Sarufutsu coast showed freshwater levels (0.5–4.0 × 10−3) near the core, which thereafter increased to brackish water levels (4.0–6.0 × 10−3), and then to seawater levels (6.0–10.0 × 10−3) in the outermost regions. Those findings indicate that specimens from the Sarufutsu coast migrated to the brackish water
region or the sea and spent most of their lives there. The anadromous migration pattern including the timing of downstream
migration seems to be flexible among individuals in the species. They migrate between freshwater and seawater or brackish
water several times during their lives, showing extensive habitat use. It is essential to secure the continuity among the
freshwater, brackish water, and seawater areas for their effective conservation. 相似文献
6.
Md. Shah Alam Sarker Shuichi Satoh Keisuke Kamata Yutaka Haga Yoshihiro Yamamoto 《Aquaculture Research》2012,43(4):538-545
A feeding experiment was conducted to investigate the effect of organic acids and/or lipid supplementation on growth, utilization and environmental loading of nitrogen (N) and phosphorus (P) in juvenile yellowtail fed fishmeal (FM) and plant protein (PP) diets. Six diets as FM (FM‐based), FM+P (FM with inorganic P), FM+L (FM with lipid), PP+CA (PP with citric acid), PP+L+CA (PP with lipid and citric acid) and PP+L+FA (PP with formic acid) were formulated. Yellowtails were fed each of the diets in duplicate groups; once a day, 6 days a week to near satiation at water temperature 19.0–25.0 °C for 16 weeks. Fishmeal with inorganic P gave the best growth while PP+L+FA the lowest. However, growth increased in PP+CA and PP+L+CA. Addition of lipid significantly increased N and P retention resulting in significant reduction in N and P excretion. Citric acid and FA supplementation to PP diets also increased retention of P; hence, its excretion was lowered. Thus, CA, FA and lipid in juvenile yellowtail diets can help to partially replace FM with PP sources and reduce inorganic P use to minimize environmental loading from aquafeeds. 相似文献
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Evaluating the durability of wood-based panels using internal bond strength results from accelerated aging treatments 总被引:1,自引:0,他引:1
In this study, the durability of wood-based panels was evaluated by comparing the internal bond (IB) strength retention after
five different laboratory-based accelerated aging tests with the IB retention after 5 years of outdoor exposure in Shizuoka
City. In each accelerated aging test, the IB retention of MDI-bonded panels showed high retention compared to other panels.
Outdoor exposure in Shizuoka City resulted in an IB retention value for particleboard (PF) and oriented strandboard (aspen)
of less than 10% after the 5-year exposure period. Medium-density fiberboards maintained their initial IB strength over the
same period. Calculation of the mean IB retention for all board types allowed comparison of the severity of aging between
the accelerated test methods and outdoor exposure. The ASTM six-cycle test method was the most severe among the standard treatment
cycles applied. 相似文献
9.
Kuroishi T Komine K Kai K Itagaki M Kobayashi J Ohta M Kamata S Kumagai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(8):899-906
To investigate the pathological role of staphylococcal enterotoxins (SEs) and toxic shock syndrome toxin-1 (TSST-1) in bovine mastitis, the production of SEs and TSST-1 was investigated in staphylococci isolated from 120 mammary gland secretions (MGS, 51 from no clinical sign-mammary glands and 69 from staphylococcal mastitic-mammary glands) collected from dairy farms where staphylococcal mastitis frequently occurred in Miyagi and Yamagata prefectures from 1997 to 1998. Concentrations of these toxins and specific antibody titers in each MGS were also measured. Furthermore, SEC and TSST-1 were inoculated into lactating mammary glands and inflammatory responses were analyzed. A high percentage of staphylococci including Staphylococcus aureus and coagulase-negative staphylococci isolated from both no clinical sign- and mastitic-MGS produced both SEC and/or TSST-1. The concentration of SEC increased with the severity of the mastitis, and was significantly higher (P<0.05) in acute mastitic-than in no clinical signs-MGS. Titers of specific antibodies to TSST-1 in MGS were significantly higher (P<0.05) than those to SEC, regardless of whether or not the cows were lactating or mastitic. Specific antibodies purified from MGS neutralized each toxin in vitro. A significant increase (P < 0.05) in somatic cell counts was induced by the intramammary inoculation of SEC but not TSST-1. These findings indicated that SEC rather than TSST-1 plays an important role in the pathology of staphylococcal bovine mastitis. The inflammatory activity of TSST-1 was probably neutralized by specific antibodies in MGS. 相似文献
10.
Kentaro Okuno Tomoko Hama Minoru Takeshita Naruto Furuya Yoichi Takanami 《Journal of General Plant Pathology》2003,69(2):138-142
A potyvirus, for which the name Japanese hornwort mosaic virus (JHMV) is proposed, was isolated from Japanese hornwort plants
(Cryptotaenia japonica) with mosaic disease symptoms. The virus was used to inoculate mechanically 34 plants belonging to 33 species of 10 families.
Of these species seven from two families were infected. Faint chlorotic spots appeared on the inoculated leaves of Chenopodium quinoa and C. amaranticolor, but no systemic infection occurred in these plants. JHMV systemically infected only Umbelliferae plants; they did not infect
26 other species in eight families. JHMV was transmitted in a nonpersistent manner by aphids (Myzus persicae). The virus was a flexuous rod-shaped particle about 750 nm in length. Sequencing the nucleotides in the 3′ terminal region
of JHMV revealed that the coat protein contains 280 amino acids with a molecular mass of 32.2 kDa. The nucleotide sequence
of the coat protein of JHMV had the highest similarity with that of Zantedeschia mosaic virus (83.3%) compared to those of
other potyviruses (57.0%–64.9%). An antiserum against JHMV reacted strongly with JHMV and weakly with Potato virus Y. These results indicate that JHMV is a new potyvirus.
Received: September 9, 2002 / Accepted: November 7, 2002
RID="*"
ID="*" The nucleotide sequence determined in this work appears in the DDBJ/EMBL/GenBank nucleotide sequence databases with
the accession number AB081518 相似文献