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Kaori KASUGA Maho HIGASHI Takahisa YAMADA Toshie SUGIYAMA Yukio TANIGUCHI Hiroaki IWAISAKI Kosuke HOMMA Yuuichi WAJIKI Yoshinori KANEKO Satoshi YAMAGISHI 《Animal Science Journal》2012,83(1):83-87
The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR‐based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W‐ and Z‐linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR‐based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR‐based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis. 相似文献
2.
Saeid Moharramipour Kazuyoshi Takeda Kazuhiro Sato Hideya Yoshida Hisaaki Tsumuki 《Euphytica》1999,106(2):181-185
The inheritance of gramine indole alkaloid was studied in F1 and F2 generations derived from crosses between wild ( Hordeum
vulgare subsp. spontaneum) and cultivated ( H. vulgare subsp. vulgare) barley at adult stage in the field. The means of gramine
content were very low in F1s and F2s in April and May. The ratio of progeny containing gramine to those without gramine (near
zero or undetectable) fit a mono-genic or di-genic model in F2 generation. However, the content of gramine might be controlled
by minor genes in the gramine-containing plants. The direction of dominance was toward lower gramine content. The averages
of dominance ( h/d) in F1 and F2 ranged from partial to complete. Broad-sense heritabilities were high (0.70 to 0.77). The
usefulness of hybrids for breeding aphid resistance and possibility increasing palatability of grazing pasture for sheep and
cattle is discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Merchant SS Prochnik SE Vallon O Harris EH Karpowicz SJ Witman GB Terry A Salamov A Fritz-Laylin LK Maréchal-Drouard L Marshall WF Qu LH Nelson DR Sanderfoot AA Spalding MH Kapitonov VV Ren Q Ferris P Lindquist E Shapiro H Lucas SM Grimwood J Schmutz J Cardol P Cerutti H Chanfreau G Chen CL Cognat V Croft MT Dent R Dutcher S Fernández E Fukuzawa H González-Ballester D González-Halphen D Hallmann A Hanikenne M Hippler M Inwood W Jabbari K Kalanon M Kuras R Lefebvre PA Lemaire SD Lobanov AV 《Science (New York, N.Y.)》2007,318(5848):245-250
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella. 相似文献
4.
Non‐invasive sampling technique for DNA extraction from captive Japanese Crested Ibis on Sado Island
Kensuke URANO Takahisa YAMADA Yukio TANIGUCHI Hiroaki IWAISAKI Toshie SUGIYAMA Kosuke HOMMA Yoshinori KANEKO Satoshi YAMAGISHI 《Animal Science Journal》2011,82(4):616-619
The Japanese Crested Ibis Nipponia nippon is a critically threatened bird. The post‐hatch eggs of the current captive population of this species on Sado Island have been stored at room temperature for the long‐term. In this study, we investigated the suitability of the vascularized chorioallantois membrane from the eggs as a non‐invasive DNA source. Using microsatellite loci developed for the Japanese Crested Ibis, we performed three experiments for comparison of genotypes obtained among DNA. First, DNA from five different sites of the identical membrane showed the same genotypes at either of two loci examined. Second, DNA from the membrane of each full‐sibling birds and blood of their parents showed the genotypes that were consistent with Mendelian parent–offspring relationships at any of eight loci examined. Third, DNA from the membrane and blood of the same bird showed the matched genotypes at any of eight loci examined. These results indicate that the vascularized chorioallantois membrane from post‐hatch eggs stored at room temperature for the long‐ term can be used as a reliable DNA source of offspring that had hatched from the egg. This study will promote a molecular genetics study on genetic diversity of the current captive Japanese Crested Ibis population on Sado Island. 相似文献
5.
Effects of a commercial probiotic, Bacillus cereus toyoi on abdominal fat accumulation in the Japanese quail fed either a commercial control diet (crude protein (CP), 23.5%; metabolizable energy (ME), 11.7MJ/kg) or a high‐energy diet (CP, 23.0%; ME, 13.8MJ/kg) were investigated. Four‐week‐old male birds (n = 108) were divided into four diet groups: control diet, control with probiotic, high‐energy diet and high‐energy diet with probiotic. At 8 weeks and 12 weeks of age (4 and 8 weeks of probiotic supplementation period, respectively), abdominal fat, muscles and the liver were weighed. The weight of total fat and protein in the muscles and liver were also determined. A rectal temperature was recorded weekly. Bodyweight and feed intake in both diets were not affected by probiotic supplementation. At 8 weeks of age, birds fed the control diet with probiotic had significantly less abdominal fat than those fed without the probiotic, and a similar tendency was seen for both diet groups at 12 weeks of age. The decrease in abdominal fat coincided with an increase in rectal temperature. These results suggest that greater metabolizable energy was consumed through elevation of heat production by supplementation of B. cereus toyoi, with hardly any energy stored as body fat. Probiotic supplementation appears to induce greater muscle weight, higher protein and lower lipid content in muscles by 8 weeks of age, and lower lipid content in muscles and greater liver weight by 12 weeks of age, in the Japanese quail. 相似文献
6.
Kotaro Mori Hideya Tokuoka Hisashi Miyagawa Yoshiaki Nakagawa 《Journal of Pesticide Science》2021,46(1):120
Benzoylphenylureas (BPUs) were discovered as novel type insecticides about a half century ago; many analogs have been launched as insecticides and acaricides. BPUs are known to inhibit chitin synthesis in insects and other arthropods, but they have no effect against microorganisms such as fungi. We designed new chitin synthesis inhibitors based on the hypothesis that biomolecules that play important roles in cellulose and chitin biosynthesis are similar. In the full automatic modeling system (FAMS), the cellulose synthase was selected as a template three-dimensional structure. Thus, we focused on the structure of cellulose synthase inhibitor, isoxaben, to develop new chemistry. The 1,1-diethylethyl [-C(CH3)(CH2CH3)2] group of isoxaben was changed to a 4-substituted phenyl group bearing Cl, Et, or Ph. These compounds significantly inhibited chitin synthesis in the cultured integument of the rice stem borer Chilo suppressalis. The activity of the 4-ethylphenyl analog was enhanced 30-fold by adding piperonyl butoxide to the culture medium. 相似文献
7.
Effects of erect panicle genotype and environment interactions on rice yield and yield components
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WANG Yuan-zheng Olusegun IDOWU WANG Yun HOMMA Koki NAKAZAKI Tetsuya ZHENG Wen-jing XU Zheng-jin SHIRAIWA Tatsuhiko 《农业科学学报》2023,22(3):716-726
The dense and erect panicle (EP) genotype conferred by DEP1 has been widely used in the breeding of high-yield Chinese japonica rice varieties. However, the breeding value of the EP genotype has rarely been determined at the plant population level. Therefore, the effects of the interaction of EP genotype and the environment at different locations and times on rice yield and its various components were investigated in this study. Two sets of near-isogenic lines (NILs) of EP and non-EP (NEP) genotypes with Liaojing 5 (LG5) and Akitakomachi (AKI) backgrounds were grown in the field in 2016 and 2017 in Shenyang, China, and Kyoto, Japan. In 2018, these sets were grown only in Kyoto, Japan. The average yields of the EP and NEP genotypes were 6.67 and 6.13 t ha−1 for the AKI background, and 6.66 and 6.58 t ha−1 for the LG5 background, respectively. The EP genotype positively affected panicle number (PN) and grain number per square meter (GNPM), mostly resulting in a positive effect on harvest index (HI). In contrast, the EP genotype exerted a negative effect on thousand-grain weight (KGW). The ratio of the performance of the EP genotype relative to the NEP genotype in terms of yield and total biomass correlated positively with mean daily solar radiation during a 40-day period around heading. These results indicate that the effectiveness of the EP genotype depends on the availability of solar radiation, and the effect of this genotype is consistently positive for sink formation, conditional in terms of source capacity, and positive in a high-radiation environment. 相似文献
8.
Atsutoshi KUWANO Hidekazu NIWA Tohru HIGUCHI Hideya MITSUI Robert A. AGNE 《Journal of Equine Science》2012,23(3):41-46
A 3-year-old thoroughbred colt presented with canker on its left hind foot. Subsequent
development of cottage cheese-like horns and dermatitis disturbed healing, despite the use
of miscellaneous orthodox treatment approaches to the lesions. Histological examination
revealed exudative and suppurative dermatitis, and proliferatively suppurative epidermitis
infected with helically coiled treponemes. Total debridement under general anesthesia led
to a temporary improvement, but the ground surface regenerated abnormal epidermis similar
to that observed initially after surgery. Maggot debridement therapy (MDT) was attempted,
which removed all the abnormal tissue. After MDT, general farriery trimming helped to
correct the distorted ground surface, and the horse returned to constant training and
eventually raced. This case shows that MDT was successfully used for treatment of an
intractable and treponemes-infected canker. 相似文献
9.
Rosalina M. LAPITAN Arnel N. DEL BARRIO Osamu KATSUBE Tomomi BAN-TOKUDA Edgar A. ORDEN Alberto Y. ROBLES Tsutomu FUJIHARA Libertado C. CRUZ Hideya HOMMA Yukio KANAI 《Animal Science Journal》2007,78(6):596-604
This study was conducted to determine and compare the live traits, carcass yield and the physical, chemical, processing and sensory properties of meat from 20 Brahman grade cattle (crossbred cattle) and crossbred water buffalo (Philippine carabao × Murrah, Bulgarian or Indian) with an average age and weight of 29 months and 434 kg, respectively. The animals were fed a similar diet for 180 days before slaughter. A survey was performed to establish consumer meat preference between the two species. Live weights before slaughter of the two species were found to be different (P < 0.05) with the crossbred water buffalo showing higher bodyweight. However, the crossbred water buffalo had a lower (P < 0.05) dressing yield based on hot carcass weight and chilled carcass weight. The lower dressing yield of crossbred water buffalo can be attributed to its higher (P < 0.05) weights of edible and non‐edible slaughter by‐products. The chilled carcass yield of the forequarters and hindquarters from crossbred cattle and crossbred water buffalo were comparable when expressed as percent of live weight and chilled carcass weight. The estimated lean yield was higher in crossbred cattle than crossbred water buffalo. Crude protein, ash, fat, cholesterol, myofibrillar, sarcoplasmic and insoluble protein contents of the beef and carabao meat were all similar. Water holding capacity, pH, muscle fiber diameter, tenderness, firmness and marbling score in carabeef were all comparable to the beef. Redness was found to be higher (P < 0.05) in carabeef than the beef as measured with a color meter. The consumer meat preference survey showed that 55.9% selected the beef while 44.1% preferred carabeef. The color and amount of fat on the outside of the beef sample were primary in buyer selection. 相似文献
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