甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析

为了进一步研究甜菜谷胱甘肽转移酶BvGSTU9 (LOC104894060)在重金属胁迫过程中的功能。本研究以‘780016B/12优’为实验材料,对该基因序列特征、结构、功能进行预测分析,并利用qPCR检测该基因在不同浓度镉胁迫下的表达量变化。结果显示甜菜BvGSTU9基因全长925 bp,开放阅读框675 bp,编码了由224个氨基酸组成的不稳定膜外蛋白。BvGSTU9与菠菜、藜麦的氨基酸序列相似性较高,与系统发育进化树分析结果基本相符。二级和三级结构预测表明该基因主要由α-螺旋、β-折叠、延伸链及无规则卷曲组成。qPCR显示BvGSTU9基因在不同浓度的镉胁迫下均受到不同程度的诱导,因此可以推断甜菜BvGSTU9基因无论从结构还是功能上,与镉逆境胁迫存在着一定的应答关系。研究结果也为甜菜耐重金属镉机制研究提供参考依据。     

连续施用有机肥对黄瓜体内Zn的积累及其在亚细胞分布的影响

以黄瓜品种'津优35号'为试材,采用田间随机区组试验设计,研究了不同用量(0、15、30、45 t·hm-2和60 t·hm-2)鸡粪源有机肥中Zn在土壤中的积累,以及Zn在黄瓜体内的吸收转运及其在亚细胞各组分中的分布情况,以期为指导黄瓜的安全生产以及鸡粪有机肥合理施用提供参考依据.结果 表明:低用量鸡粪有机肥施用就能显著提高土壤Zn含量(P＜0.05),土壤Zn含量最高为140.40 mg·kg-1,均未超出国家安全标准.施用60 t·hm-2鸡粪有机肥显著提高了黄瓜根部和果实Zn含量,黄瓜果实Zn含量变化范围为19.27～23.59 mg·kg-1,但Zn含量均未超过100 mg·kg-1,可以安全食用.无论鸡粪施用量的高低,Zn在黄瓜根、茎、叶和果实各亚细胞组分中均表现为可溶性组分＜细胞器(叶绿体和线粒体)＜细胞壁.60 t·hm-2鸡粪有机肥可以显著提高黄瓜根部细胞壁Zn含量,且根部细胞壁中Zn含量及所占比例随着鸡粪有机肥施用量的增加而增加.细胞壁中Zn在根中所占比例最高,达到了54.67％～58.77％.     

废纸回收材料化利用研究进展

简述了国内外废纸回收利用现状,重点阐述了废纸在建筑填料、制备高附加值纤维衍生物以及复合材料等方面的利用研究现状,并针对废纸材料利用过程中存在的高吸水率和弱结合界面等问题提出了相应的解决方法和研究方向。     

国内高架桥绿化及研究现状

从高架桥区的生态环境、适生种类的选择以及高架桥绿化的养护管理、景观配置模式四个方面对国内的高架桥绿化及研究现状进行了分析和探讨,指出了存在的问题和解决方法,并对高架桥绿化的前景进行了展望.     

几种分析方法对杂种棉后代综合评价的比较研究

为了明确几种分析方法对杂种棉后代综合评价的异同，于2002-2003年通过聚类分析、关联度分析以及多目标综合值评定分析原理对10个杂种棉后代在不同生态点的主要农艺及经济性状进行了分析．结果表明：3种分析方法对杂种棉后代的综合评价趋势基本上一致．从纤维品质来看，以湘Z-010，湘Z-004，湘Z-008，湘Z-007纤维品质较好．从产量性状来考虑，以湘Z-001，湘Z-002，湘Z-004产量较高．从湖南定位50-60支纱区划方针和产量性状来看，以湘Z-004表现最好，属于高产优质品种．     

纤维亚麻新品种黑亚15号选育报告

黑亚15号是2004年2月通过黑龙江省农作物品种审定委员会审定通过同意注册的纤维亚麻新品种。原茎、长麻、全麻、种子产量分别为5641．7、897．2、1282．3、619．okg／hm2，分别比对照增产9．3％、20．2％、12．2％、11．9％。长麻率20．O％，比对照高2．8个百分点。纤维强度261．ON。属高纤、优质纤维亚麻新品种。     

哈尼族传统人居环境体系中森林要素之作用评述

从不同角度评述了森林在哈尼族传统人居环境体系中的作用和地位，阐述了未来山区村镇发展中保留周边森林的重要性。     

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.     

Relationship between expression of somatostatin and DNA ploidy in breast cancer

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer.