奶牛繁殖疾病的调控

本文综述了奶牛繁殖疾病的发病原因和调控措施，分析认为奶牛的营养状况是影响奶牛繁殖力的重要因素。理想的奶牛管理应做到整个泌乳期始终监测奶牛的体况和奶牛日粮中微量元素的维生素的含量，避免营养素的不平衡而引发的奶牛繁殖障碍，同时应避免奶牛过肥或过瘦。适当补充某些维生素和矿物质十分重要。     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

Chloride channels of platelets

Chloride channels distribute widely in the body, and participate in many physiological actions and regulatory processes. Based on their physiological roles and molecular structures, six kinds of chloride channels have been identified: (1) The chloride channels family; (2) Cystic fibrosis transmembrane conductance regulator; (3) Swelling-activated chloride channels; (4) Calcium-activated chloride channels; (5) The p64 (CLIC) gene family; (6) γ-aminobutyric acid and glycine receptors. The chloride channels do exist in platelets, and their appearances are dependent on the presence of intracellular calcium. Blocking agents of chloride channels inhibit the thrombin-activated platelet aggregation and the elevation of the intracellular calcium concentration in a dose-dependent manner. It is suggested that chloride channels play a role in the activation of platelets. In addition, chloride channels act on both the cell volume regulation and the intracellular pH regulation in platelets.     

滇中云南松火烧后第一年死亡预测模型研建

根据云南松（Pinus yunnanensis）的耐火性和受火害后遗留下来容易获取的信息建立火烧死亡预测模型,可以为林火熄灭后短时间内进行火灾损失评估、迹地植被恢复方案设计等提供理论依据。在安宁2006年“3·29”森林火灾火烧迹地上对发生过树冠火的云南松死活情况、火烧状况进行了调查,测定了286株云南松的树高、胸径、胸高处树皮平均厚度、树干烧黑高度、树干表面圆周烧黑宽度、树干基部树皮平均炭化深度,记录了受害木的流脂状况。以树高、胸径、胸高处树皮平均厚度、树干纵向最大烧黑率、树干弦向最大烧黑率、树干基部树皮径向平均炭化率和流脂状况7个指标作为预测依据,运用学习向量量化人工神经网络建立滇中云南松火烧死亡预测模型,并用C^＋＋实现了该模型。经检验,该模型的平均准确率达81．12％,对死树的预测正确率达93．72％,对活树的预测正确率达48．10％,可以用于云南松火烧后第一年死亡的判别和预测。     

杂交晚稻机插不同播量条件下育插秧试验研究

以杂交晚稻“江四优992”为供试材料,进行了不同播量软盘育秧机插与常规水育秧手插的对比试验。结果表明：机插软盘育秧随播种量增加,漏插率降低,以90～100g芽谷／盘为最佳播量;机插秧虽然前期分蘖稍慢,但后期具有分蘖稳健、无效分蘖少、成穗率高的特点;机插稻的全生育期比手插稻延长2～3d;与手插稻相比,机插稻的穗粒结构合理,穗粒数多,产量高,平均增产10．2％。     

不同处理方法对重楼种子萌发的Meta分析

应用Meta分析方法,通过中英文数据库筛选了1990—2019年关于重楼(Paris polyphylla)种子萌发的文献24篇,得到78个独立试验组,分析温度、机械、生长素、复合处理对重楼种子萌发的影响,并探讨较高海拔下重楼种子繁育的可能性。结果表明,恒温处理促进重楼种子萌发是一种简单可靠的方法,恒温、变温、沙藏方式的交互处理能够有效提高重楼种子发芽率(相对发芽率为0.61)。生长素使用不当会抑制重楼种子萌发。在相同处理条件下,滇重楼[Paris polyphylla Sm. var. yunnanensis(Franch.)Hand.-Mazz]比华重楼[Paris polyphylla Sm. var. chinese(Franch.)H. Hara]具有更好的发芽效率和稳定性。针对适宜重楼生长的较高海拔生境下种子发芽相关研究缺乏,需要针对较高海拔重楼繁育技术进行研究。     

锡林郭勒盟春季沙尘暴成因的气象因子分析

本文选取了锡林郭勒地区沙尘暴高发季节春季(3~5月)的大风日数、气温、相对湿度等气象因子,通过对当地气象因子的调查,采用基于因子分析的主成分提取方法,将气象因子分为3个主成分,并分析了各主成分的意义,通过对沙尘暴成因的各种气象因素所作的定量和定性分析,在沙尘暴的预报和监测中具有重要的实际意义。     

Response of CREG1 promoter to serum starvation in human vascular cells

AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression.     

利用SSR标记分析45份爆裂玉米自交系遗传多样性

利用SSR分子标记分析了45份爆裂玉米自交系的遗传多样性。结果表明:所选取SSR引物的扩增带型均清晰稳定。利用80对SSR引物在45份爆裂玉米自交系间共检测出334个等位基因变异,多态性信息量(PIC)变化为0.10~0.83,平均为0.53。其PIC为0.4~0.8,以0.6~0.7的标记数为最多,占标记总数的20.96%。本试验结果说明爆裂玉米种质具有较高遗传多样性。根据SSR数据,计算分子标记遗传距离,供试自交系遗传距离(GD)为0.095~0.931,平均为0.512。通过聚类分析,以遗传距离0.55为标准,供试自交系可划分为5个类群。群间遗传距离均大于群内遗传距离,分类合理。     

温度对百合种球催芽生根的影响

以‘西伯利亚’、‘索邦’和‘木门’3个百合品种种球为试验材料,采用泥炭为基质进行箱栽,种植后把种球置于10 ℃、12 ℃和14 ℃的低温冷库中进行催芽生根处理,以直接栽培于大棚条件下的种球为对照,处理14d后,测定各处理组的种球鲜质量变化、芽长、茎生根数量、生根率和芽粗。结果表明,12℃下处理‘西伯利亚’、‘索邦’和‘木门’种球种植14d的生根效果最好,可显著促进茎生根的发生和芽的生长,且整齐度较好。