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排序方式: 共有201条查询结果,搜索用时 9 毫秒
1.
When crude enzymes prepared from some vegetables and fruits were incubated with bisphenol A (2,2-bis(4-hydroxyphenyl)propane, BPA) at 37 degrees C, BPA was oxidized by crude enzymes from potato, eggplant, and lettuce. The crude enzyme prepared from potato (Solanum tuberosum) had the strongest oxidative activity for BPA. Its optimal temperature and pH were 40-45 degrees C and 8.0, respectively. More than 95% of BPA was oxidized after the incubation with potato enzyme for 60 min. BPA gave two oxidation products besides insoluble compounds during the oxidation by potato enzyme. The oxidation products were identified to be 4[1-(4-hydroxyphenyl)-1-methyl-ethyl]-benzene-1,2-diol and 4[1-(4-hydroxyphenyl)-1-methyl-ethyl]-benzene-1,3-diol. Enzymatically oxidized BPA lost the estrogen-like activity to enhance the growth of human breast cancer (MCF-7) cells. 相似文献
2.
Suzu Sakao Takafumi Fujimoto Terumasa Kobayashi Goro Yoshizaki Etsuro Yamaha Katsutoshi Arai 《Fisheries Science》2009,75(4):993-1000
Diploid gametes generated with tetraploid animals are a stepping stone to improving chromosome manipulation techniques. However,
artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could be induced by in vivo
cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs
were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they
migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole-mount in situ hybridization using vasa messenger RNA (mRNA), 4–35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological
observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia.
They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that
inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and
tetraploid fish production. 相似文献
3.
Poxvirus infection in Nile crocodiles (Crocodylus niloticus) 总被引:1,自引:0,他引:1
G S Pandey N Inoue K Ohshima K Okada Y Chihaya Y Fujimoto 《Research in veterinary science》1990,49(2):171-176
An outbreak was encountered of numerous yellowish cutaneous nodules in one- to two-year-old farmed Nile crocodiles (Crocodylus niloticus) in Kasaba Bay Crocodile Farm at Lake Tanganyika in Zambia during 1988. Out of 4000 crocodiles of different age groups, 300 yearlings were affected and 82 of those affected died. The lesions were prominent on the head, especially around the eyelids, the nostrils, both sides of the mouth, ventral neck, ventral pale belly, limbs and the root of the tail. Histologically, the epidermal lesions revealed large focal areas of marked acanthosis accompanied by hyperkeratosis and parakeratosis. In the prickle cells, there were multiple small neutrophilic granular inclusions and large eosinophilic homogeneous cytoplasmic inclusions. Electron microscopically, there were numerous poxvirus particles and matrices in the cytoplasm of many prickle cells. Dark blue homogeneous cytoplasmic inclusions in toluidine blue sections consisted of an electron opaque matrix with mature viral particles (160 x 200 x 230 nm). Furthermore, there were clumps of granular matrix containing immature viral particles (200 x 399 nm) in the cytoplasm. Various features of viral developing processes were observed. 相似文献
4.
Fujimoto Kiyoshi Miyagi Toyohiko Kikuchi Takao Kawana Toshio 《Mangroves and Salt Marshes》1996,1(1):47-57
Mangrove habitats on Kosrae are divided into three types, i.e., an estuary or delta type, a backmarsh or lagoon type and a coral reef or tidal-flat type. Most of the mangrove forests of Kosrae have been developed during the last 2000 years by accumulating mangrove peat with the gradual sea-level rise of 1 to 2 mm/yr except the landward part of the estuary or delta type. On the other hand, during the period of rapid sea-level rise of about 10 mm/yr between 4100 and 3700 yr B.P., the mangrove forests ceased peat accumulation and retreated landward. Until 3500 yr B.P., mangrove forests were distributed only in narrow bands in the inlets. Therefore, the critical rate of mangrove peat accretion with sea-level rise is estimated at more than 2 mm/yr and less than 10 mm/yr. If the anticipated sea-level rise exceeds this critical rate, all of the mangrove forests of Kosrae will retreat landward and reduce rapidly. 相似文献
5.
Takaaki Fujimoto Kazuhito Kita Kazuko Uchiyama Makoto Kuromaru Hisashi Akutsu Kazuyuki Oda 《Journal of Forest Research》2006,11(3):157-163
Age trends in variance components and heritability of overall wood density, earlywood and latewood density, and latewood proportion
were investigated in 29-year-old trees of 19 full-sib families of hybrid larch (Larix gmelinii var. japonica × Larix kaempferi) F1. The age–age correlation and optimum selection age for these traits were also estimated and genetic and phenotypic correlations
between wood density and radial growth rate were calculated for each growth ring. Intraring wood density data were obtained
using X-ray densitometry. The coefficient of additive genetic variance was stable over all ages, whereas the coefficient of
environmental variances gradually decreased with increasing age, resulting in increases in heritability estimates with age
for overall density. The latewood proportion had the highest heritability estimates at all ages, ranging from 0.44 to 0.66.
Overall density and its various components at 28 years of age showed strong genetic correlations with their respective traits
at all younger ages. Optimum selection ages for the wood density traits ranged from 8 to 14 years, at which point maximum
gain efficiencies per year were obtained. There were negative correlations between wood density and radial growth rate at
early ages, although these relationships tended to be weaker with increasing age. These results suggest that selection at
a young age is effective for wood density, but particular care must be taken in selecting trees with an improved radial growth
rate because rapid growth will result in a low-density wood product, especially in the early growth period. 相似文献
6.
Quantitative determination of cyproconazole by gas chromatography–mass spectrometry (GC/MS) analysis was investigated. The results suggest that cyproconazole determination is significantly affected by wood extracts. In the presence of methanol extracts of Japanese cedar (Cryptomeria japonica) heartwood, cyproconazole showed 50 % larger peak area than that without the extracts. This phenomenon whereby contaminants in the sample solution affect the intensity of the peak signal is known as the matrix effect. An investigation for eliminating the matrix effect revealed that adding sorbitol as an analyte protectant drastically increased the analyte peak intensity and successfully mitigated the matrix effect of the wood extracts. Adding hexaconazole as an internal standard was also useful in reducing the deviation of the data. The efficacy of a novel sample preparation method consisting of adding sorbitol and hexaconazole as analyte protectants and the internal standard, respectively, were applied to the GC/MS analysis of cyproconazole in the presence of wood extracts of the other nine species. It is revealed that cyproconazole can be precisely determined by GC/MS analysis in combination with the analyte protectant and the internal standard. 相似文献
7.
8.
Murahashi T Fujimoto M Oka MA Hashimoto Y Uemura T Tatsumi Y Nakao Y Ikeda A Sakaki S Kurosawa H 《Science (New York, N.Y.)》2006,313(5790):1104-1107
Despite the abundance of "sandwich" complexes, in which two cyclic aromatic hydrocarbon ligands flank a metal center, this motif has not been extended to sheets of multiple metal atoms. We prepared and isolated two such compounds. In the first, three palladium centers form a planar triangular array, capped by chlorides, between two cycloheptatrienyl ligands. In the second, a pentapalladium sheet adopts an edge-sharing triangle-trapezoid skeleton between two naphthacene rings. The compounds were characterized by x-ray crystallography and nuclear magnetic resonance spectroscopy. The nature of bonding in the clusters was analyzed by quantum calculations. 相似文献
9.
Hideaki Endo Eiji Takahashi Masataka Murata Hitoshi Ohnuki Huifeng Ren Wakako Tsugawa Koji Sode 《Fisheries Science》2010,76(4):687-694
A wireless biosensor system was developed for the continuous measurement of blood glucose levels in flatfish. The biosensor
was implanted in the interstitial fluid under the scleral surface of the eyeball (EISF) to investigate the relationship between
EISF and blood glucose levels. EISF glucose levels were found to be correlated with those in the blood and to be approximately
the same as blood glucose levels in the range of 7–25 mg dl−1. A needle-type biosensor was prepared for the continuous EISF glucose monitoring in flatfish. A working electrode was constructed
using platinum iridium wire, and glucose oxidase was immobilized to the electrode. The biosensor was inserted into the EISF
of flatfish for sensor implantation. A 650-mV potential (vs. Ag/AgCl) was applied by a wireless potentiostat to the working
electrode for the amperometric glucose measurement. We investigated whether glucose in the EISF can be determined in vivo.
The estimated glucose levels using a one-point calibration method were correlated with actual blood glucose levels. In conclusion,
using a wireless biosensor system, we were able to monitor blood glucose levels in flatfish under free-swimming conditions
for 16 h. 相似文献
10.
Isolation of influenza A viruses from migratory waterfowl in San-in District, western Japan in the winter of 1983-1984 总被引:1,自引:0,他引:1
K Otsuki O Takemoto R Fujimoto K Yamazaki N Kubota H Hosaki T Mitani M Tsubokura 《Research in veterinary science》1987,43(2):177-179
Certain species of winter migratory waterfowl in San-in District, western Japan, were surveyed for influenza virus from November 1983 to March 1984. Faeces of the waterfowl were collected regularly at five stations. Eleven influenza A viruses including H5N3 and H10N4 subtypes were isolated from 450 faecal samples from whistling swans, 28 viruses including H2N2 and H10N4 subtypes were isolated from 362 faecal samples from pintails; and subtype H13N6 was isolated from 240 faecal samples of black-tailed gulls. 相似文献