Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria (Molina) Standl.on NDV in vitro

To research on polysaccharides from Lagenaria siceraria (Molina) Standl.against Newcastle disease virus (NDV).Total polysaccharide and four different solvent extractions of Lagenaria siceraria (Molina) Standl.were extracted by the methods of hot water extraction and ethanol precipition in this study.The content of polysaccharide was measured by phenol-sulfuric acid method and infrared spectroscopy method.The safe concentration and growth of chick embryo fibroblast (CEF) were assayed by MTT method,in order to facilitated the comparison under the same level,the safe concentration was united as 78.125 μg/mL.Under the safety range of concentration,detected the block-virus activity,anti-virus activity and virus-killing activity of polysaccharides through the ways of pre-adding polysaccharides,post-adding polysaccharides and adding polysaccharides with NDV.The results showed that the direct inactivation and propagation inhibition activity of total polysaccharide and four different solvent extractions were stronger than anti-absorption function.Anti-virus inhibition rate of 70%,80% gradient alcohol precipitation of polysaccharides from Lagenaria siceraria (Molina) Standl.(LSP₇₀,LSP₈₀) and total polysaccharide (LSP_t)were 40.41%,44.54% and 61.85%,virus-killing inhibition rate were 44.74%,58.76% and 59.38%.LSP₈₀ had the highest virus inhibition rate as 37.14% in the block-virus activity of those five polysaccharides.In summary,70%,80% gradient alcohol precipitation and total polysaccharide in polysaccharides possessed better activity and would be as the materials for further research.     

阿魏根粗多糖对鸡脾脏和外周血淋巴细胞体外增殖作用的影响

为研究新疆阿魏多糖（FSP）对鸡外周血T淋巴细胞和脾脏B淋巴细胞增殖作用的影响。本研究用水提醇沉法得到阿魏总多糖（FSP_t）和3种分级多糖（FSP₃₀、FSP₅₀、FSP₇₀）,并通过苯酚-硫酸法和红外光谱对FSP进行检测。通过MTT法测定各级FSP的安全浓度及其对鸡外周血T淋巴细胞及脾脏B淋巴细胞的增殖能力。结果显示,提取的FSP均具有多糖的特征峰,为多糖类化合物。外周血T淋巴细胞增殖试验表明,除FSP₅₀外,所有多糖在一定浓度下均能单独和协同PHA刺激T淋巴细胞增殖,其中FSP_t的增殖作用较强。脾脏B淋巴细胞增殖试验表明,除FSP₅₀外,所有多糖在一定浓度下均能单独和协同LPS刺激B淋巴细胞增殖,其中FSP₇₀和FSP_t增殖作用较强。综上所述,各级FSP中,FSP_t对脾脏B淋巴细胞和外周血T淋巴细胞的增殖作用最强,可以作为进一步研究的材料。     

Effects of Polysaccharides from Ferula sinkiangensis Root on Proliferation of Chicken Peripheral Lymphocyte and Spleen Lymphocyte in vitro

To investigate the effects of Ferula sinkiangensis polysaccharides (FSP) on cell proliferation of chicken splenic lymphocytes and peripheral lymphocyte in vitro. By the method of water extract-alcohol precipitation,total Ferula sinkiangensis polysaccharide (FSP_t) and 30%,50%,70% gradient alcohol precipitation of polysaccharides (FSP₃₀,FSP₅₀ and FSP₇₀) were extracted, and detected by phenol-sulfuric acid method and infrared spectroscopy method. The safe concentration of all classifications of polysaccharide were detected and the effect of FSP on lymphocyte proliferation of chicken spleen and peripheral blood were measured by MTT method. The results showed that the absorption peak of FSP were conformed to belong by polysaccharide compounds. Peripheral blood T lymphocyte proliferation test indicated that FSP_t,FSP₃₀ and FSP₇₀ alone or cooperated with PHA could stimulate T lymphocyte proliferation under a certain concentration,and FSP_t showed a better proliferation effect. Spleen B lymphocyte proliferation test showed that FSP_t,FSP₃₀ and FSP₇₀ alone or cooperated with LPS could stimulate B lymphocyte proliferation under a certain concentration,FSP_t and FSP₇₀ showed a better proliferation effect. In conclusion,FSP showed the strong effect on lymphocyte proliferation of chicken spleen and peripheral blood in vitro,and that would be the materials for further research.     

葫芦多糖体外抗鸡新城疫病毒作用的研究

为研究葫芦多糖抗新城疫病毒（Newcastle disease virus,NDV） 活性,本试验采用水提醇沉法提取葫芦总多糖及4种分级多糖,通过苯酚-硫酸法及红外光谱对葫芦多糖进行检测,MTT法测定各级多糖对鸡胚成纤维细胞（CEF）的安全浓度,多糖的安全浓度统一定为78.125 μg/mL,以3种加药方式（先加多糖后接种病毒、先接种病毒后加多糖、多糖和病毒感作后加药）,检测葫芦多糖对NDV的阻断、抑制及直接杀灭作用。结果表明,葫芦总多糖及4种分级多糖均具有抗NDV活性,其中对NDV的抑制作用及直接杀灭作用强于对NDV的阻断作用。70%、80%梯度醇沉的葫芦多糖（LSP₇₀、LSP₈₀）及总多糖（LSP_t）,抑制作用下的病毒抑制率分别为40.41%、44.54%、61.85%,杀灭作用下的病毒抑制率分别为44.74%、58.76%和59.38%,阻断作用下对NDV的抑制率,其中LSP₈₀组最高达37.14%,其余各组均低于25%。综上可知,70%、80%梯度醇沉的葫芦多糖及葫芦总多糖的抗NDV活性较好,可作为进一步研究的材料。