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Detection of bacterial contamination and DNA quantification in stored blood units in 2 veterinary hospital blood banks 下载免费PDF全文
Valentina Stefanetti Arianna Miglio Katia Cappelli Stefano Capomaccio Elisa Sgariglia Maria L. Marenzoni Maria T. Antognoni Mauro Coletti Vittorio Mangili Fabrizio Passamonti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):406-410
Blood transfusions in veterinary medicine have become increasingly more common and are now an integral part of lifesaving and advanced treatment in small and large animals. Important risks associated with transfusion of blood products include the transmission of various infectious diseases. Several guidelines suggest what infectious agents to screen for in canine and feline transfusion medicine. However, while the risk of bacterial contamination of blood products during storage and administration has not been documented in veterinary medicine, it has emerged as a cause of morbidity and mortality in human transfusion medicine. Clinical experience shows that the majority of blood component bacterial contaminations are caused by only a few species. Unlike other types of bacteria, psychrotolerant species like Pseudomonas spp. and Serratia spp. can proliferate during the storage of blood units at 4°C from a very low titer at the time of blood collection to a clinically significant level (> 105 CFU/mL) causing clinical sepsis resulting from red blood cell concentrate transfusions in human medicine. The purpose of this report was to describe the detection and quantification procedures applied in 4 cases of bacterial contamination of canine and feline blood units, which suggest the need for further investigations to optimize patients’ safety in veterinary transfusion medicine. 相似文献
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Riccardo Trentin Luísa Custdio Maria Joo Rodrigues Emanuela Moschin Katia Sciuto Jos Paulo da Silva Isabella Moro 《Marine drugs》2022,20(3)
This work studied the potential biotechnological applications of a naviculoid diatom (IMA053) and a green microalga (Tetraselmis marina IMA043) isolated from the North Adriatic Sea. Water, methanol, and dichloromethane (DCM) extracts were prepared from microalgae biomass and evaluated for total phenolic content (TPC) and in vitro antioxidant properties. Biomass was profiled for fatty acid methyl esters (FAME) composition. The DCM extracts had the highest levels of total phenolics, with values of 40.58 and 86.14 mg GAE/g dry weight (DW in IMA053 and IMA043, respectively). The DCM extracts had a higher radical scavenging activity (RSA) than the water and methanol ones, especially those from IMA043, with RSAs of 99.65% toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) at 10 mg/mL, and of 103.43% against 2,2-diphenyl-1-picrylhydrazyl (DPPH) at 5 mg/mL. The DCM extract of IMA053 displayed relevant copper chelating properties (67.48% at 10 mg/mL), while the highest iron chelating activity was observed in the water extract of the same species (92.05% at 10 mg/mL). Both strains presented a high proportion of saturated (SFA) and monounsaturated (MUFA) fatty acids. The results suggested that these microalgae could be further explored as sources of natural antioxidants for the pharmaceutical and food industry and as feedstock for biofuel production. 相似文献
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Wanessa Blaschi Paula A. Lunardelli Luciana S.R. Marinho Marilu C. Max Gustavo M.G. Santos Katia C. Silva-Santos Fabiana A. Melo-Sterza Hernan Baldassarre Thales R. Rigo Marcelo M. Seneda 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):433-437
Synchronization of estrus and ovulation are of paramount importance in modern livestock improvement programs. These methods are critical for assisted reproduction technologies, including artificial insemination and embryo transfer, that can increase productivity. In the current study, subcutaneous implants containing norgestomet were placed for long (14 days), medium (9 days), and short (5 days) periods of time in 70 crossbred ewes undergoing fixed-time artificial insemination. The resulting effects on estrus synchronization and conception rates were subsequently evaluated. Among the synchronized ewes, 85.7% (60/70) underwent estrus over a period of 72 h after progestagen treatment ceased. The shortest mean interval between withdrawal of the device and onset of estrus (34.2 ± 8.9 h) was observed in the G14 days of P4 group (p < 0.05). The conception rate of the G14 days of P4 group was statistically higher than that of the other groups (83.3% vs. 60.9% vs. 47.8%; p < 0.05). In conclusion, 14 days of norgestomet treatment produced higher conception rates and a greater number of pregnancies at the beginning of the breeding season. 相似文献
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Kawasaki PM Kano FS Tamekuni K Garcia JL Marana ER Vidotto O Vidotto MC 《Research in veterinary science》2007,83(3):347-354
Anaplasmosis, caused by Anaplasma marginale, results in significant economic losses of cattle in tropical and subtropical regions worldwide. Six major surface proteins (MSPs) were well characterized and designated as MSP1, MSP2, MSP3, MSP4, and MSP5. The objective of this study was to evaluate the humoral immune response of BALB/c mice against the recombinant MSPs, incorporated into immunostimulating complex (ISCOM). The recombinant proteins purified by Ni-NTA columns were incorporated into ISCOM and ISCOMATRIX by the lipid film hydration method. BALB/c mice immunized with ISCOM/rMSPs and ISCOMATRIX/rMSPs vaccines produced whole IgG, IgG1, and IgG2a, in contrast to the negative groups (PBS and ISCOMATRIX adjuvant). All groups that received antigen responded specifically against the rMSPs by Western blotting, showing the rMSP1a (60-105kDa), rMSP1b (100kDa), rMSP4 (47kDa), and rMSP5 (29kDa). Additional studies will have to be performed in cattle to evaluate the humoral and cellular mechanisms of this subunit vaccine and their possible use as protective vaccines against homologous and heterologous strains of A. marginale. 相似文献
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Cunha SC Aguero C Damico CB Corgozinho KB Souza HJ Pimenta AL Marassi CD 《Journal of Feline Medicine and Surgery》2011,13(3):205-207
CLINICAL SUMMARY: A 7-month-old female Persian cat presented with gastrointestinal (GI) necrosis and perforation caused by Rhizomucor species. Unfortunately, the cat died of bacterial peritonitis and sepsis before a definitive diagnosis, based on histopathology and fungal culture, was achieved. PRACTICAL RELEVANCE: This appears to be the first reported case of GI disease caused by Rhizomucor species in a cat. Mucorales infections typically cause acute and rapidly progressive disease. As illustrated by this case, clinicians should be alert to the potentially fatal consequences of an opportunistic Rhizomucor species infection in their feline patients. 相似文献
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Katia Duquesne Aurlie Lieutaud Jeanine Ratouchniak Andrs Yarzbal Violaine Bonnefoy 《European Journal of Soil Biology》2007,43(5-6):351
Weathering of the arsenopyrite-rich tailings of the Carnoulès abandoned mining site (Gard, France) results in the formation of acid mine waters heavily loaded with arsenic. Dissolved arsenic present in the seepage waters is precipitated within a few meters from the bottom of the tailing dam by microorganisms. From these effluents, we have isolated and characterized a Gram negative bacterium involved in this bioremediation process. This bacterium, which belongs to the Thiomonas genus, is very efficient in removing arsenite from contaminated acidic waters by oxidizing it to arsenate, which precipitates. 相似文献
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Corgozinho KB de Souza HJ Pereira AN Belchior C da Silva MA Martins MC Damico CB 《Journal of Feline Medicine and Surgery》2007,9(6):481-486
Fifteen cats were evaluated with urethral obstruction. Penile trauma by catheterization was the major indication for perineal urethrostomy. Ten cats had developed a urethral stricture and five had rupture of the urethra following medical management. All cats had abnormalities in penis and/or prepuce and/or scrotal sacs including hyperemia or swelling. Perineal urethrostomy was performed in all cases and they were evaluated for 6 months after surgery. Few complications were noted. Urinary tract infection was the most frequent complication observed. The clients considered their cats to have a good quality of life following surgery. 相似文献
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Marc Vandeputte Mathilde Dupont-Nivet Pierrick Haffray Hervé Chavanne Silvia Cenadelli Katia Parati Marie-Odile Vidal Alain Vergnet Béatrice Chatain 《Aquaculture (Amsterdam, Netherlands)》2009,286(1-2):20-27
Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and separate tanks, both selected groups (P, M) were larger than unselected groups (W, D). No difference was seen at any time between W and D, nor between M and P. The selection response estimate on weight was larger in mixed tanks when compared to separate tanks (+ 42% in mixed tanks, + 23% in separate tanks at day 611), yielding realized heritability estimates of 0.60 and 0.34, respectively, and confirming the excellent potential of the species for growth improvement through selective breeding. Both selection response and the amplification effect between mixed and separate tanks decreased as rearing density increased. Our hypothesis is that selection response is magnified by competition in mixed tanks, while sub-optimal rearing conditions lower the observed selection response, more in separate tanks (where selected thus larger fish are at a higher density than unselected ones) than in mixed tanks (where all fish experience the same density effects). 相似文献