粟品种对粟瘟病的抗病性初步观察

近年来,粟瘟病在山西省中部及东南部严重发生危害,輕者减产,重者籽粒不收。作者于1962—1963年进行了粟品种間抗病性的初步观察,簡报如下: 在本所試驗地和山西省晋东南区各县良种繁殖場谷子品种区域試驗地共調查品种520个。調查时期:重点品种分苗期、拔节期、拔节末期、抽穗盛期、乳熟期进行;一般品种只在发病盛期(即抽穗盛期)进行。在自然条件下未感染粟瘟病的品种,再在溫室中种植,进行人工接种。抗病性鑑定結果如下: (一)尚未发現有免疫品种。 (二)不同品种对栗瘟病的抗病性有显著差异:在发病盛期調查,高度抗病品种61个,占总数12％,发病指数为0.1—10;抗病品种149个,占总数28.7％,发病指数为10.1—20.0;輕度感病品种128个,占总数24.6％;发病指数为20.1—     

液力变矩器导轮流场数值模拟与试验

利用CFD分析软件对W305型液力变矩器的内部流场进行了仿真计算。采用混合平面理论处理旋转速度不同的各叶轮之间的相互作用。基于仿真计算结果,重点分析了导轮内流场的速度和压力分布情况,并将导轮内流场计算结果与试验结果进行了对比分析,结果表明流场计算结果具有较高的精度。     

黔东南小香鸡的品种遗传资源特点及开发利用

目的探究日粮添加谷氨酰胺(glutamine,Gln)对黔东南小香鸡免疫器官指数及肠道免疫的影响。方法选取90羽体质量相近、健康状况良好的1日龄黔东南小香鸡,随机分为3组,每组5个重复,每个重复6羽。对照组饲喂基础日粮,试验组在饲喂基础日粮中分别添加0.5% 和1.0%的Gln。饲养至42 d,每组随机选取10羽屠宰,采集血清,分离免疫器官,刮取空肠肠黏膜,用于后续测定。结果与对照组相比,0.5% Gln组黔东南小香鸡脾脏指数显著升高(P<0.05),1.0% Gln组的法氏囊指数、脾脏指数、胸腺指数、血清IgA、IgG和IgM含量及空肠黏膜sIgA分泌均显著升高(P<0.05),并且空肠黏膜的MUC1和MUC2 mRNA的相对表达量也显著增加(P<0.05)。结论黔东南小香鸡日粮添加1.0% Gln可促进免疫器官发育,增强肠道黏膜免疫功能。     

基于LF-NMR及MRI 的2种速生材干燥过程中水分分布状态研究

为了解太阳能干燥过程中速生材内部水分状态及迁移规律,采用低场核磁共振及核磁共振成像检测桉、杨木干燥过程中水分分布、含量变化及迁移过程,借助扫描电镜在微观层面进行分析。结果表明：核磁共振信号强度与称重法所测含水率相关性系数高达0.99以上,因此低场核磁共振可准确测定木材中的水分含量;桉木和杨木饱水试样的核磁共振信号中均存在3种峰,桉木弛豫时间分别为1.32、32.75、403.70 ms,杨木为2.66、32.75、352.12 ms,对应着结合水及两种状态的自由水;随着干燥进行,不同状态水分的横向弛豫时间逐渐减小,木材对水分的束缚增加。当桉木含水率为27.28%,杨木含水率为35.71%左右时,水分散失转为以结合水为主。通过核磁共振成像中氢质子密度的不同可对水分含量进行区分,进而可观测干燥过程中木材内部水分移动状态。因此,通过低场核磁共振及核磁共振成像可研究速生材干燥过程中的内部水分状态及分布。     

Modeling waterbird diversity in irrigation ponds of Taoyuan, Taiwan using an artificial neural network approach

The study develops an approach adopted by artificial neural networks (ANN) to model the relationship between pondscape and waterbird diversity. Study areas with thousands of irrigation ponds are unique geographic features from the original functions of irrigation converted to waterbird refuges. The model considers pond shape and size, neighboring farmlands, and constructed areas in calculating parameters pertaining to the interactive influences on avian diversity, among them the Shannon–Wiener diversity index. Results indicate that irrigation ponds adjacent to farmland benefited waterbird diversity. On the other hand, urban development leads to the reduction of pond numbers, which reduces waterbird diversity. By running the ANN model, the resulting index shows a good-fit prediction of bird diversity against pond size, shape, neighboring farmlands, and neighboring developed areas with a correlation coefficient (r) of 0.72, in contrast to the results from a linear regression model (r < 0.28).     

小麦产量性状与粒重性状的遗传分析

以小麦6044×01-35杂交后获得的重组自交系群体为试材,对其产量性状与粒重性状予以遗传分析。结果表明,重组自交系群体产量性状均呈正态分布,有不同程度的变异;单穗粒重与穗长和千粒重呈极显著正相关,与单株穗数、行总粒重呈极显著负相关,与株高呈显著负相关;广义遗传力较高的为株高、穗下节间长、穗长、单株粒重和单穗粒重;由主成分分析可知,对穗粒重贡献最大的是单株粒重、穗下节间长、穗长、单株穗数;通过逐步回归分析和通径分析,单株粒重对单穗粒重有促进作用,直接通径系数最大,单株穗数对单穗粒重的负效应最高。     

Adaptability of various models of the water production function for rice in Jilin Province,China

The existing irrigation schedule of most rice-growing areas in Jilin Province is not in full compliance with the Water Requirements Law of the rice; these discrepancies affect the yield of rice to some extent. Thus, research on rice water regulations and selection of an appropriate water production function model (WPFM) is very important. In this study, according to the water demand characteristics of rice, we used drought treatments separately during the tillering, jointing booting, flowering, and milky stages. Evapotranspiration and yield data were obtained using a test pit experiment and production measurements in 2011 and 2012, respectively. Five commonly used WPFMs (the Jensen, Blank, Minhas, Stewart, and Singh model) were selected for this study, and the water sensitivity index of each model was calculated based on the evapotranspiration, yield, and the model equations. The Jensen model was found to be the most suitable WPFM for this region; furthermore, a mathematical relationship between the water sensitivity index and days after rice planting was established. These results have important theoretical and practical implications for guidelines regarding rice irrigation in Jilin Province.     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

常见动物乳与人乳的营养成分比较分析

乳中富含多种人体所必需的营养成分,对机体的生长发育及免疫功能具有重要作用。除牛乳外,水牛乳、马乳、骆驼乳、山羊乳、驴乳、牦牛乳等特色乳也逐渐进入人们的视野。同时人乳是婴儿最天然的理想食品,是婴儿早期主要的营养物质来源,含有丰富的营养物质及生物活性成分。为了更好的比较7种常见动物乳及人乳的差异性和优势性,本文总结了其在乳糖、乳脂、乳蛋白、矿物质、维生素等营养物质成分方面的差异,发现牦牛乳的蛋白质含量最高,氨基酸种类丰富;马乳的乳糖含量最高;而骆驼乳的乳脂肪含量则较高,水溶性维生素含量也最高;驴乳相比于其他几种常见乳,则具有低脂高糖等特点。     

Immunopathogenic behaviors of canine transmissible venereal tumor in dogs following an immunotherapy using dendritic/tumor cell hybrid

Canine transmissible venereal tumor (CTVT) is a naturally occurring tumor that can be transmitted between dogs via live tumor cell inoculation. It is also a spontaneous self-regression tumor and its behavior is closely related to host immune responses. Since CTVT had been widely used for tumor models in canine cancers, whether this self-regression may overtake the immunity elicited from an exogenous tumor vaccine remains unclear and certainly worthwhile to be investigated. In this study, we used DCs/tumor hybrids as a tumor vaccine to evaluate the CTVT model. We prepared mature allogeneic dendritic cells from bone marrow and then assessed their phenotype (CD80, CD83, CD86, CD1a, CD11c, CD40 and MHC II), antigen uptake and presenting abilities. Fused dendritic cell/CTVT hybrids were then used as a vaccine, administered three times at two-week intervals via subcutaneous injection near the bilateral auxiliary and inguinal lymph nodes. In comparison with unvaccinated dogs (spontaneous regressed group), within a period of 2.5 months, the vaccinations substantially inhibited tumor progression (p<0.05) and accelerated the rate of regression by a mechanism involving amplification of the host tumor-specific adaptive immune responses and NK cytotoxicity (p<0.001). Pathologic examination revealed early massive lymphocyte infiltration resulting in final tumor necrosis. In addition, there are not any detectable effects on routine physical, body temperature or blood chemistry examinations. In conclusion, our data furnishes a reference value showing that CTVT is a model of potential use for the study of immunity elicited by vaccines against tumors, and also enable early-phase evaluation of the dendritic cell/tumor vaccine in terms of raising host immunity.