全文获取类型
收费全文 | 22242篇 |
免费 | 1287篇 |
国内免费 | 2291篇 |
专业分类
林业 | 2251篇 |
农学 | 2330篇 |
基础科学 | 1937篇 |
3219篇 | |
综合类 | 7962篇 |
农作物 | 1316篇 |
水产渔业 | 1114篇 |
畜牧兽医 | 3383篇 |
园艺 | 1123篇 |
植物保护 | 1185篇 |
出版年
2024年 | 124篇 |
2023年 | 370篇 |
2022年 | 880篇 |
2021年 | 1060篇 |
2020年 | 989篇 |
2019年 | 1003篇 |
2018年 | 660篇 |
2017年 | 1088篇 |
2016年 | 772篇 |
2015年 | 1103篇 |
2014年 | 1082篇 |
2013年 | 1303篇 |
2012年 | 1660篇 |
2011年 | 1735篇 |
2010年 | 1553篇 |
2009年 | 1433篇 |
2008年 | 1360篇 |
2007年 | 1291篇 |
2006年 | 1190篇 |
2005年 | 936篇 |
2004年 | 507篇 |
2003年 | 443篇 |
2002年 | 432篇 |
2001年 | 396篇 |
2000年 | 383篇 |
1999年 | 334篇 |
1998年 | 229篇 |
1997年 | 221篇 |
1996年 | 215篇 |
1995年 | 191篇 |
1994年 | 141篇 |
1993年 | 142篇 |
1992年 | 122篇 |
1991年 | 122篇 |
1990年 | 83篇 |
1989年 | 70篇 |
1988年 | 56篇 |
1987年 | 39篇 |
1986年 | 34篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 9篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1956年 | 4篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
1.
石灰性土壤的电超滤(EUF)特性主要是浸提时因需控制电流而必须大大降低电压。在一定范围内,降压程度与土壤 CaCO_3%有密切关系。控流限额愈低,电压需成比例地下降,但土壤EUF-值减小不多。电流控制在一定值时,离子的解吸总量亦为定值。控流主要控制了各石灰性土壤解吸/溶解份额特大的 Ca+Mg 量近于定值,对于含量小的其他养分离子则并无多大影响,仍可区辨各土壤间的差别。通过冬小麦盆栽试验和有关化学测试法等方面的相关研究,表明采用国际通用的5g 土样和控电流方法测定 EUF-K 和-P 等有效养分,对于石灰性土壤也是可行的,但其EUF-(Ca+Mg)值则毫无植物营养有效性的意义。土壤 EUF 值只是一种指数,并非真正的解吸离子量。石灰性土壤 EUF-K 值因浸提时降压控流而略低,80℃时5min 内解吸的钾不一定是非交换性钾。由于 Ca-P 和 CaCO_3在高温高电压时浸出较多,故石灰性土壤 EUF-P 和-(Ca+Mg)的80℃值/20℃值之比,与酸性土壤或非石灰性土壤相比要大得多。 相似文献
2.
3.
分泌抗鸡Ig单抗杂交瘤细胞系的建立与单抗生物学特性的鉴定 总被引:7,自引:0,他引:7
从传染性法氏囊病病毒(IBDV)单克隆抗体(McAb)杂交瘤细胞系1D10、1G1中得到了3株能稳定分泌抗鸡免疫球蛋白(Ig)McAb的杂交瘤细胞系1B7、1D7、3G6。其抗体类和亚类均属IgG2b,腹水的ELISA效价≥1∶25600,琼扩效价为1∶8~1∶16。3株McAb能与鸡血清中的Ig出现沉淀反应,琼扩在24h以内出现清晰的沉淀线,而不能和鸭、鸽、鹌鹑等禽类及异种动物血清出现沉淀线。纯化的鸡IgG、IgM经SDS-PAGE后,分别用纯化并经辣根过氧化物酶(HPR)标记的1B7、1D7、3G6单抗酶结合物进行免疫印迹试验证明,3株单抗识别的均为IgG、IgM分子的轻链 相似文献
4.
张彬 《湖南农业大学学报(自然科学版)》1990,(1)
用经改进的聚丙烯酰胺凝胶电泳铁染法,测定了二花脸猪血浆转铁蛋白多态类型,分析了其基因型频率和基因频率,并探讨了猪血浆转铁蛋白多态性与产仔性能和增重速度的相关性。 相似文献
5.
韩占兵 《河南畜牧兽医(综合版)》2001,22(11):7-7,48
14只2岁非洲黑鸵鸟按公母比例1:2~3分成4个繁殖组合,对产蛋、受精、孵化情况进行观察分析。结果显示,每只母鸵鸟年平均产蛋量为55.2枚,最高约为73枚,最低为39枚。4个组合鸟群之间种蛋的受精率存在显著的差异P<0.01,I组的受精率最高,为59.6%;IV组的受精率最低,仅8.9%。各组的受精率、孵化率差异不显著P>0.05,平均受精蛋孵化率达到92.6%。本次受精率较低的原因为公鸟年龄小,没有完全达到性成熟。 相似文献
6.
Kyungmin Han Inge Geurden Paul Van der Meeren Sungchul C. Bai Patrick Sorgeloos 《Journal of the World Aquaculture Society》2005,36(2):196-202
The present study examined the distribution of particle sizes in two experimental standardized lipid emulsions (distributed by ICES, International Council for the Exploration of the Sea) as a function of the preparation method (hand shaking or ultrasonic blending) and as a function of storage time. A 24-h enrichment trial compared the incorporated HUFA levels in the nauplii of Artemia franciscana .
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles. 相似文献
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles. 相似文献
7.
Paymon Roustaian Mohd Salleh Kamarudin Hishamuddin Bin Omar Che Roos Saad Mansor Haji Ahmad 《Journal of the World Aquaculture Society》2001,32(1):53-59
Abstract.— Quantitative changes in the protein, lipid and carbohydrate were studied in the early larval stages of developing freshwater prawn Macrobrachium rosenbergii reared under fed and starved conditions to understand the relative importance of these nutrients in energy metabolism of the growing larvae. Larvae obtained from several females were stocked into three 250-L tanks at a density of 30 larvae/L. The feeding regime consisted of newly hatched Artemia nauplii. Protein was always the major organic constituent followed by lipids and then carbohydrates of both fed and starved larvae. Protein levels of both fed and starved larvae increased during development, suggesting an important role in morphogenesis. The decline of lipid during the larval growth that was more rapid for starved larvae, suggests a probable utilization of lipid as the major metabolic source of energy. Carbohydrates formed less than 5 and 2.4% of the larval dry weight of fed and starved larvae, respectively, suggesting their limited role in larval metabolism. 相似文献
8.
9.
Y Sugawara K Hirasawa M Takeda J S Han K Doi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(3):463-468
One-month-old Syrian hamsters of the APA and Std: golden strains were inoculated intraperitoneally with 10(5) PFU/head of the D variant of encephalomyocarditis (EMC) virus and examined virologically and pathologically up to 7 days after inoculation. APA hamsters developed apparent hyperglycemia due to pancreatic islet cell damage while Std:golden hamsters did not. Hamsters of both strains showed clear histopathologic changes in the testis with prominent viral replication as well as in the brain, heart and exocrine pancreas. The susceptibility to EMC virus-infection was higher in males than in females and in APA than in Std: golden hamsters. 相似文献
10.