Foot and mouth disease and bluetongue: differences and similarities

On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures.     

Drainage and vertical hydraulic conductivity of some Dutch “knik” clay soils

The vertical K-sat of a clay layer, occurring between 30 and 60 cm below the soil surface, was measured in situ in early spring at thirteen sites, using large soil columns. Gypsum was used to form a barrier around the column and K-sat values were measured with an infiltrometer in columns that were first attached and then detached from the subsoil. This procedure allows an estimate of the occurrence of large continuous pores, such as vertical worm channels. Highest values were found in tile-drained grassland, followed by grassland with surface drainage only, and by tile-drained arable land. Relatively low K-sat for the silty subsoil, rather than the (high) vertical K-sat for the clay layer, is considered to be responsible for high groundwater tables in the wet season.Undisturbed, large columns were taken to the laboratory and saturated for a period of three months to simulate prolonged swelling after a very wet season, and to measure chloride-breakthrough curves, for characterizing soil-pore continuity. The clay layer, sampled in the surface-drained grassland, showed no significant reduction of K-sat after prolonged swelling, but the one for arable land was reduced. Moreover, flow in the latter occurred through only a few relatively large, continuous pores, whereas a more heterogeneous pore system was found for the column from grassland. The already high K-sat of the clay layer in surface-drained grassland increased as a result of tile drainage. Compaction of the clay layer in tile-drained arable land reduced K-sat well below the level found in surface-drained grassland.     

Genes related to flooding tolerance during germination and early growth of weedy rice

Evolution of flooding tolerance in weedy rice has occurred in several rice-growing regions, but the genes related to this process and the environmental effects are unknown. The objective of this study was to analyse the expression of genes related to flooding tolerance in response to temperature and flooding during the initial establishment of weedy rice. The experiments were carried out with rice cultivars IRGA 417 and Nipponbare, which are sensitive to flooding, and weedy rice ITJ03 and AV04 genotypes that have high and intermediate tolerance to flooding, respectively. The expression of genes related to reserve mobilisation, anaerobic respiration, escape and quiescence strategies was analysed at periods up to 24 days after sowing. The flooding tolerance of weedy rice genotype ITJ03 was associated with the expression of RAmy3D and OsTPP7 , which are involved in the mobilisation of carbohydrate reserves, ADH1 and ADH2, which participate in anaerobic respiration, and SNRKL1 that triggers rapid elongation of the coleoptile and emergence. Although the genes PDC1, SUS3 and SUB1 are important for flooding tolerance in cultivated rice, their expression was not directly related to flooding tolerance in weedy rice. A temperature of 20°C reduced levels of expression of the RAmy3D, ADH2 and SNRKL1 genes and low temperature had a negative effect on the establishment of weedy rice. Breeding of rice genotypes with tolerance of low temperatures and anaerobic conditions may be a viable strategy to improve the control of weedy rice in paddy fields.     

The effect of soil texture and organic amendment on the hydrological behaviour of coarse-textured soils

To gain more insight into the hydrological behaviour of coarse-textured soils, the physical properties of artificially created soil mixtures with different texture were determined. The mixtures were prepared according to the specifications of the United States Golf Association (USGA) for constructing putting greens. In addition, the effect of 10 vol.% organic matter addition was studied. The soil moisture retention and hydraulic conductivity relationships of the different mixtures were determined and their hydrological behaviour was studied using the numerical model SoWaM. Both texture and organic matter addition substantially affected the hydraulic properties. Hydraulic conductivity significantly increased with increasing coarseness while moisture retention decreased. On the other hand, organic matter addition reduced saturated hydraulic conductivity by a factor of 10 to 100 and distinctly increased moisture retention capacity. The amounts of total available water were increased by the addition of organic matter between 144% (slightly coarse texture) and 434% (very coarse texture). Results indicate that the mixtures can contain only 2–16% plant available water and therefore need frequent irrigation to maintain plant growth. Addition of organic matter seems a good solution to reduce the irrigation water requirements but it increases the risk of ponding or runoff because of large reductions in the saturated hydraulic conductivity sometimes to below the rate of 3.6 m/day recommended by the USGA.     

Comparison of radiographic assessments of the tibial plateau slope in dogs

OBJECTIVES: To evaluate the accuracy of 2 radiographic methods used to assess tibial plateau slope (TPS) in dogs and evaluate effects of film digitization and radiographic beam placement on TPS measurements. SAMPLE POPULATION: 16 hind limbs from dog cadavers weighing > 20 kg. PROCEDURES: Radiographs of tibiae were made with the radiographic beam centered over the stifle joint and midshaft of the tibia. Tibiae were collected, the femorotibial contact area was determined, and slope of the medial tibial condyle in relation to the tibial shaft was measured. Radiographs were digitized. Slope of the medial tibial condyle was measured on printed and digitized radiographs read in random order by 6 examiners unaware of anatomic measurements. Three examiners used a conventional measuring technique, and 3 examiners used an alternative measuring technique. RESULTS: Anatomic measurements were significantly higher than radiographic measurements made by use of the conventional interpretation method but did not differ from radiographic measurements made by use of the alternate method. Measurements from printed radiographs were lower than measurements from digitized radiographs for the 4 most experienced examiners. CONCLUSIONS AND CLINICAL RELEVANCE: Measurements made by use of a line tangential to the cranial, linear portion of the medial tibial condyle at the femorotibial contact point were accurate measurements of the anatomic TPS. Measurements made by use of the conventional TPS measurement method underestimated the anatomic TPS. Measurements made on digitized radiographs were typically more accurate than measurements made on printed radiographs.     

Attract,reward and disrupt: responses of pests and natural enemies to combinations of habitat manipulation and semiochemicals in organic apple

The widespread use of pesticides along with the simplification of the landscape has had undesirable effects on agroecosystems, such as the loss of biodiversity and the associated ecosystem service biological control. How current production systems can be remodelled to allow for a re-establishment of biological pest control, while preserving productivity, is a major challenge. Here, we tested whether a combination of tools could augment or synergize biological control of insect pests in apple (Malus domestica), comprised of a tortricid pest complex, a geometrid pest complex and the rosy apple aphid. The tools aimed at disrupting mating behaviour of multiple pest species (multispecies mating disruption, “Disrupt”, MMD), attracting natural enemies (a blend of herbivory-induced volatiles, “Attract”, A), or providing refuge and rewards for a diverse insect community (perennial flower strip, “Reward”, R) over a 3-year period. Suction samples were consistently richer in generalist predators but not in parasitoids when multiple tools including MMD?+?A?+?R or MMD?+?A were employed. In addition, lepidopteran pest levels were significantly lower in these plots than in MMD or MMD?+?R at the end of the 3-year experiment. This was, however, not reflected in survival of artificially established aphid colonies. Our data indicates that multiple, complementary tools can greatly enhance natural enemy level, but also that long-term implementation is needed to fully realize the augmentatory or synergistic potential of complementary components and restore biological control as an ecosystem service of practical relevance.

     

Prevalence of Campylobacter jejuni and Salmonella during pig slaughtering

It was found that 79% of healthy pigs, slaughtered in three different slaughterhouses in the Netherlands, were intestinal carriers of Campylobacter jejuni (mean number 4000 cfu per g), and 21% of the same pigs had Salmonella in the intestinal tract (mean number 10 cfu per g). Immediately after slaughter, Campylobacter was swabbed from 9% of the carcasses and Salmonella from 13%. It is concluded from these data that most of the contamination on carcasses does not originate directly from the intestinal tracts of the animals but rather from surfaces, equipment, and utensils in the slaughter hall. It was demonstrated that Salmonella could survive in the slaughter hall, whereas Campylobacter died off, probably due to its vulnerability to drying conditions and its inability to grow at temperatures below 30 degrees C. Campylobacter was not isolated from the carcasses after cooling. It had been shown earlier that this again was caused by dry conditions, brought about by the use of forced ventilation in the cooling rooms. In an additional investigation, Campylobacter was not isolated from 248 samples of minced pork (10 g each), whereas Salmonella was found in 13% of these samples.