Exophthalmos Caused by Chronic Sinusitis in a Horse: A Case of Exophthalmos Caused by Sinusitis

A 12-year-old Thoroughbred gelding exhibited a unilateral exophthalmos on the left side. There had been no other clinical symptoms, such as rhinorrhea, before the first consultation. Clinical, hematologic, plasma biochemical, cytologic, and computed tomography (CT) examinations and complete necropsy were performed. These examinations did not reveal neoplastic lesions. CT images suggested that maxillary and frontal sinuses may have been filled with fluid. Cytologic and pathologic examinations revealed that the maxillary and orbital bones were pushed up with a large amount of the inflammatory fluid effused by chronic sinusitis. The CT and cytologic examinations were of great use for differential diagnosis in this case. This was an unusual case of exophthalmos induced by deformation of the orbital bones resulting from chronic sinusitis.     

Three-dimensional live imaging of bovine embryos by optical coherence tomography

While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. Four of the seven recipients became pregnant. We suggest that these 22 parameters can be potentially employed to evaluate the quality of bovine embryos before ET.     

Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice

Recently, developmental exposure to clothianidin (CLO) has been shown to cause reproductive toxicity in male mice, but the effects in female mice remain to be clarified. Pregnant C57BL/6N mice were given a no-observed-adverse-effect-level (NOAEL) dose of CLO until weaning. We then examined ovaries of 3- or 10-week-old female offspring. In the CLO-administered group, morphological changes, a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 (GPx4), and activation of genes in the steroid hormone biosynthesis pathway were observed in 3-week-old mice, and decreases of GPx4 immunoreactivity, 17OH-progesterone and corticosterone levels were observed in 10-week-old mice, along with high rates of infanticide and severe neglect, providing new evidence that developmental exposure to CLO affects juvenile and adult mice differently.     

Effect of high frequency surface and subsurface drip irrigation on root distribution of sweet corn

Summary Characterization of root growth and distribution is fundamental in explaining crop responses to irrigation and in determining appropriate management of irrigation systems, particularly with drip systems since it is widely believed that drip irrigation may limit the extent of root development. An experiment was conducted to study root distribution of sweet corn grown under high frequency surface (S) and subsurface (SS) drip irrigation, fertilized daily through drip systems at three phosphorus levels of P₀ (no injected P), P₁ (P injected at 67 kg/ha) and P₂ (P injected at 134 kg/ha). Root sampling at the end of the growing season indicated that: (1) Root extension continued at depths in excess of 2 m in both the surface and subsurface drip at all P levels. (2) The greatest differences between SS and S treatments were observed in the top 45 cm depth. Higher root length density was observed in the surface 30 cm in S plots while the sweet corn in the SS plots had greater root length density than S plots below 30 cm, and (3) the greater root length density in the SS irrigated sweet corn was not reflected in a similar increase in total above-ground dry matter.This project was partially supported by a grant from BARD Project no I-1116-86     

Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca²⁺ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca²⁺ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.     

Chemical composition and in vitro ruminal digestibility of Ludwigia grandiflora

Ludwigia grandiflora is an amphibious plant that has been designated as a specific alien plant in Japan. Due to the risk of regeneration on land, plants are burned after eradication. Since L. grandiflora is fertile and rich in biomass, this study investigated the use of L. grandiflora as a feed for ruminants. We measured general components, detergent fiber components, and in vitro dry matter digestibility (IVDMD) using rumen microbes for three forms (terrestrial form, emergent form, and floating-leaved form) of L. grandiflora. In addition, concentrations of hazardous metallic elements were also measured from the viewpoint of feed safety. Crude protein, neutral detergent fiber, acid detergent fiber, and acid detergent lignin were contained 10.31–14.62, 27.83–42.28, 20.54–36.05, and 6.94–18.90 (%DM), respectively. IVDMD was 50.75%–68.24%. Toxic elements such as Pb, Cd, and Hg were not detected in all forms. These results suggest that L. grandiflora could be partially used as alternative roughage for ruminants.     

Ecology of pitaya stem rot caused by Gilbertella persicaria and its biological control by phyllosphere bacteria

Gilbertella persicaria stem rot is a disease that causes rotting in the stem nodules and fruits of pitaya. Here, we aimed to study the ecology of G. persicaria and its biological control using several Bacillus subtilis strains isolated from the phyllosphere. Sporangiospores of G. persicaria were detected on different parts of the plant, with maximum density (c.239.9 propagules/cm²) found on the petals after flowering, and spore germination was observed to be considerably affected by humidity. Furthermore, the study revealed that an incision wound of at least 2 mm depth was necessary for infection by the pathogen to the stem nodule, and exposure to high temperatures (50°C) accelerated disease incidence, by disrupting resistance to the spread of the pathogen. Additionally, biological control of G. persicaria was effectively achieved by inoculation with B. subtilis strains isolated from pitaya stem nodules. Intriguingly, treatment with antagonistic bacteria reduced stem rot incidence to zero (bacterial suspension: PNB-5, PNB-7, and PNB-93; culture filtrate: PNB-7, PNB-93, and PNB-95), equivalent to that achieved by fungicide application in both prophylactic and simultaneous treatments. Collectively, this is the first study to demonstrate the ecology of G. persicaria and to show the potential applicability of phyllosphere bacterial strains for biological control of G. persicaria in pitaya, which could help develop effective integrated disease management strategies for the control of pitaya stem rot.     

Detection of telomere length and oxidative stress in Chondrichthyes

Telomeres, repeating TTAGGG sequences at the ends of eukaryotic chromosomes, increase genomic stability. Telomere shortening occurs not only during DNA replication associated with cell division but also under oxidative stress, where reactive oxygen species damage DNA. Therefore, changes in telomere length can be used to evaluate chronic cost or stress responses incurred by individuals. The phylogenetically unique Chondrichthyes are among the least-studied groups of marine vertebrates. Telomere data are limited and have only been reported in a few Chondrichthyes species. In this study, we measured telomere length and quantified oxidative stress and antioxidant power in 17 Chondrichthyes species whose telomere length has not been measured before. The presence of telomere sequences?>?30 bp and lower values of oxidative stress were confirmed in most species. Average telomere length was not correlated with oxidative stress and antioxidant power in 15 species for which both measurements were available. It would be desirable in the future to elucidate the nature of telomeres in Chondrichthyes, and their direct relationship with reactive oxygen species.