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Tsuji N Ohta M Kawazu S Kamio T Isobe T Shimura K Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(12):1331-1333
DNA polymorphism in twelve starains of Eimeria tenella isolated from various places in Japan was examined using 1.l kb small subunits ribosomal RNA amplified by PCR. Genetic variation was evaluated by random amplification of polymorphic DNA (RAPD) analysis. DNA fingerprint patterns were grouped into two, indicating that at least two DNA polymorphisms exist in Japanese E. tenella strains. 相似文献
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Kabamoto S Bhagat NA Suzuki H You M Kamio T Tsuji N Claveria FG Nagasawa H Igarashi I Toyoda Y Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(4):355-359
Using macrophage scavenger receptor-A knockout (SRKO) mice, we examined the role of macrophage class A scavenger receptors (MRS-A) on the immune response and acquisition of host resistance against repeated infestation with Haemaphysalis longicornis. Except for one batch of nymphs that infested one of the SRKO (SR-/-) mice and showed no appreciable reduction in body weight, all the other groups of nymphs manifested significant decrease in body weight. Both SR-/- and wild type (SR+/+) mice showed a sustained increase in anti-tick antibody titers, but SR+/+ mice showed significantly higher titers. The IFN-gamma assayed in SR-/- mouse immune sera was substantially less compared with that in SR+/+ mice. Immune sera from SR-/- and SR+/+ mice recognized the 51 and 44 kDa, and 44 kDa proteins, respectively, of the salivary gland antigen. The difference in the level of anti-tick resistance manifested by both groups of mice may be influenced by less efficient trapping and processing of tick antigens by macrophages in mice lacking for the macrophage scavenger receptors, and consequently affected the cascade of Th1 and Th2 responses. We have thus obtained valuable data that strongly infer the role of MSR-A in enhancing host defense against repeated infestation with H. longicornis. 相似文献
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Tetsuro?Shiraishi Kohei?Ohta Akihiko?Yamaguchi Mari?Yoda Hisashi?Chuda Michiya?MatsuyamaEmail author 《Fisheries Science》2005,71(3):531-542
ABSTRACT: Final oocyte maturation and ovulation of captive chub mackerel Scomber japonicus with fully yolk-accumulated oocytes were induced by a single injection of human chorionic gonadotropin. Reproductive parameters, including spawning frequency and batch fecundity, which are required to estimate spawning biomass in pelagic fish by the daily egg production method, were analyzed. Germinal vesicle migration (GVM) occurred at 18–24 h post-injection, and the hydration and ovulation of oocytes were completed at 30 and 36 h post-injection, respectively. The results of the maturation process suggest that fish with GVM-stage ovaries captured in the daytime from the field are capable of spawning on the night following their capture. The oocytes used in the oocyte size-frequency distribution method for batch fecundity estimates should be at late GVM and more advanced stages. The results of sequential artificial insemination showed that the quality of ovulated eggs held in the ovarian lumen rapidly deteriorated as time progressed after ovulation. This indicates that the fertilization window for the ovulated eggs of chub mackerel lasts only a few hours, and spawning behavior should be performed within a few hours after ovulation in the wild population. 相似文献
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Nagai Hiroshi Shibahara Shuya Matsushima Ryoji Uchida Hajime Kanamori Makoto Nogata Yasuyuki Kamio Michiya 《Fisheries Science》2021,87(1):145-150
Fisheries Science - The solitary European sea squirt Ascidiella aspersa was introduced to northern Japan, causing dense coverage of the aquacultured Japanese scallop Mizuhopecten yessoensis. There... 相似文献
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Y Yagi T Kamio K Fujisaki S Shimizu S Nagasawa Y Terada 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):13-18
Changes in plasma concentration of lipid composition were analysed in cattle with anemia due to Theileria sergenti infection. Plasma levels of phospholipids, cholesterol, free cholesterol, high density lipoprotein cholesterol, and vitamin E decreased to 40-67% of the pre-infection levels, corresponding to the decrease of PCV due to the infection. However, no definite changes were detected in plasma level of triglyceride. By gradient centrifugation, it was confirmed that lipid components, other than triglyceride, occur in high density lipoprotein (HDL) and these decreases lowered the HDL value. There was no correlation between this phenomenon and liver function. As similar changes in lipid composition were also observed in phlebotomized calves, it was considered that this phenomenon might partially depend on the acceleration of erythropoiesis as a reaction to anemia caused by T. sergenti infection. 相似文献
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Yamada T Tochimaru N Nakasuji S Hata E Kobayashi H Eguchi M Kaneko J Kamio Y Kaidoh T Takeuchi S 《Veterinary microbiology》2005,110(1-2):97-103
Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals were examined by polymerase chain reaction. LukS and lukF genes were detected in all 48 avian and 72 porcine isolates of S. aureus. LukE and lukD genes, located in a putative staphylococcal pathogenicity island (Sapln3/Saplm3), were recognized in 44 (91.7%) of 48 avian isolates, but these genes were not detected in porcine isolates. In 297 bovine isolates collected from mastitic cow's milk and bulk milk from dairy farms in two regions, lukM and lukF-PV(P83) genes in addition to lukS-lukF and lukE-lukD genes were detected in 100 (62.5%) of the 160 isolates from Ishikawa and in118 (86.1%) of the 137 isolates from Hokkaido. When the lysogeny of S. aureus bovine isolates was examined by treatment with mitomycin C, clearing of the culture due to cell lysis was observed in 34 (91.9%) of 37 lukM-lukF-PV(P83) genes--positive isolates. In addition, we isolated a novel lukM-lukF-PV(P83)-carrying (designated phiLukM), and revealed that the lukM-lukF-PV(P83) genes were located very close to an amidase gene on the temperate phage genomes. These results suggest horizontal transmission of lukM-lukF-PV(P83) genes by temperate bacteriophages in S. aureus of bovine origin. 相似文献
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Ikadai H Nagai A Xuan X Igarashi I Tsugihiko K Tsuji N Oyamada T Suzuki N Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(4):325-328
Antibodies to Babesia caballi and Babesia equi were examined on a total of 2,019 horse serum samples that had been collected in 1971-1973 by the National Institute of Animal Health by enzyme-linked immunosorbent assay (ELISA) using recombinant proteins and by Western-blot analysis. Based on the criterion for positivity by ELISA, 5.4% (109/2,019) and 2.2% (44/2,019) had antibodies against B. caballi and B. equi, respectively. The ELISA-positive sera were further examined by Western blot; 30/109 for B. caballi and 2/ 44 for B. equi were positive for native B. caballi or B. equi, but none of them was seropositive for both infections. Based on the results of this study, further investigations should be required to survey horses that have arrived in Japan relatively recently and tick vectors of equine Babesia using ELISA with some recombinant protein, a parasite detection method in an in vitro culture of equine Babesia, and PCR testing. 相似文献
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Tetsuro Shiraishi Kumiko Okamoto Michio Yoneda Takeshi Sakai Seiji Ohshimo Shizumasa Onoe Akihiko Yamaguchi Michiya Matsuyama 《Fisheries Science》2008,74(5):947-954
The age and growth of chub mackerel Scomber japonicus collected from the East China Sea and the northern waters off Kyushu between June 2000 and June 2001 were determined by observing
the otolith surface after dipping it in xylene. The translucent and opaque zones on the otolith surface were identified, and
the number of translucent zones was counted. Monthly changes in the frequency of fish with translucent zones on the otolith
margin, and in the marginal increments, indicated that the translucent zones were formed between April and June. The seasonal
pattern of annulus formation on the otolith became clear by observing the otoliths of fish with known ages, and the otolith
formation in wild fish was consistent with that of fish with known ages. The mean gonadosomatic index of male and female fish
was high from March to May, and spawning females were observed from mid-March to mid-May. The estimated ages were 1–5 years
for males and 1–6 years for females. The von Bertalanffy growth parameters did not significantly differ between male and female.
The model was obtained as FL
t=406×{1−exp[−0.372×(t+1.68)] 相似文献