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1.
Effects of road transportation on metabolic and immunological responses in Holstein heifers 下载免费PDF全文
Hyeok‐Joong Kang In Kyu Lee Min‐Yu Piao Chae‐Won Kwak Min Jeong Gu Cheol Heui Yun Hyun‐Jin Kim Hyeon‐Ju Ahn Hee‐Bal Kim Gyeom‐Heon Kim Soo‐Ki Kim Jong‐Youl Ko Jong K. Ha Myunggi Baik 《Animal Science Journal》2017,88(1):140-148
This study examined the effects of road transportation on metabolic and immunological responses in dairy heifers. Twenty Holstein heifers in early pregnancy were divided into non‐transported (NT; n = 7) and transported (T; n = 13) groups. Blood was collected before transportation (BT), immediately after transportation for 100 km (T1) and 200 km (T2), and 24 h after transportation (AT). The T heifers had higher (P < 0.05) blood cortisol and non‐esterified fatty acid concentrations after T1 and T2 than did NT heifers. By contrast, the T heifers had lower (P < 0.05) serum triglyceride concentrations after T1 and T2 than had the NT heifers. The serum cortisol and triglyceride concentrations returned (P > 0.05) to the BT concentrations at 24 h AT in the T heifers. The granulocyte‐to‐lymphocyte ratio and the percentage of monocytes were higher (P < 0.05) after T2 in the T heifers than in the NT heifers, suggesting that transportation stress increased the numbers of innate immune cells. T heifers had higher (P < 0.01) plasma haptoglobin concentrations than NT heifers 24 h AT. In conclusion, transportation increased cortisol secretion and was correlated with increased metabolic responses and up‐regulation of peripheral innate immune cells in dairy heifers. 相似文献
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Since the discovery of penicillin in 1928 and throughout the ‘age of antibiotics’ from the 1940s until the 1980s, the detection of novel antibiotics was restricted by lack of knowledge about the distribution and ecology of antibiotic producers in nature. The discovery that a phenazine compound produced by Pseudomonas bacteria could suppress soilborne plant pathogens, and its recovery from rhizosphere soil in 1990, provided the first incontrovertible evidence that natural metabolites could control plant pathogens in the environment and opened a new era in biological control by root‐associated rhizobacteria. More recently, the advent of genomics, the availability of highly sensitive bioanalytical instrumentation, and the discovery of protective endophytes have accelerated progress toward overcoming many of the impediments that until now have limited the exploitation of beneficial plant‐associated microbes to enhance agricultural sustainability. Here, we present key developments that have established the importance of these microbes in the control of pathogens, discuss concepts resulting from the exploration of classical model systems, and highlight advances emerging from ongoing investigations. © 2019 Society of Chemical Industry 相似文献
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Myung-Hoo Kim Ji-Young Yang Santi Devi Upadhaya Hyun-Jun Lee Cheol-Heui Yun Jong K. Ha 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):151-157
The purpose of our study was to investigate changes in immunological parameters induced by weaning stress (including milk restriction) in calves. Fifteen Holstein calves were subjected to weaning at 6 weeks of age. Blood samples were collected at -14, -7, -2, 1, 3, and 5 days post-weaning (DPW; 0 DPW = 42 days). Weaning caused significant (p < 0.01) increases in the neutrophil (NE):lymphocyte (LY) ratio at 5 DPW with a significant (p < 0.05) reduction of LYs. The concentration of acute-phase proteins (haptoglobin and serum amyloid A) also increased significantly (p < 0.05) at 3 and 5 DPW compared to -2 DPW. Levels of the iron-binding protein lactoferrin decreased significantly (p < 0.05) after weaning. Serum tumor necrosis factor-α and cortisol levels were elevated (p < 0.05) at 3 DPW, while those of serum interferon-γ decreased (p < 0.05) at 1 and 3 DPW compared to levels observed before weaning. Weaning significantly (p < 0.05) decreased the percentage of CD25+ T cells in the peripheral blood. In conclusion, weaning stress affected the NE:LY ratio along with the levels of acute phase proteins, lactoferrin, cortisol, and inflammatory cytokines in the peripheral blood of calves. Weaning stress may induce an acute phase response possibly through the elevation of cortisol production and modulation of inflammatory cytokines. 相似文献
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Male hybrids from a cross between female mice of strain C57BL/6Kh and males of strain DBA/2J lived longer after injection of P815 mastocytoma cells of DBA/2 origin than did their female siblings. Responses to the histocompatibility antigen on the X chromosome of the DBA/2 strain may be involved in resistance to the tumor. When the female parent was replaced with a C57BL/6Kh carrying one of several mutations in the H-2 region, this sex effect disappeared in some of the hybrid combinations. Thus, the H-2 complex appears to be involved in the regulation of the immune response to the X-linked histocompatibility antigen in this tumor model. 相似文献
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Arash Minai-Tehrani Young-Chan Park Soon-Kyung Hwang Jung-Taek Kwon Seung-Hee Chang Sung-Jin Park Kyeong-Nam Yu Ji-Eun Kim Ji-Young Shin Ji-Hye Kim Bitna Kang Seong-Ho Hong Myung-Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):309-317
Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Akt1 KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery. 相似文献
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Using two drought resistant wheat (Triticum aestivum L.) cultivars, ‘Changwu134’ and ‘Changhan58,’ a field experiment was conducted in ChangWu Agro-ecological Experiment Station on China's Loess Plateau during 2008 and 2009 to compare the effects of different fertilizer and tillage treatments on the fructan contents as well as fructan exo-hydrolase (FEH) activity in relation to wheat yield. We found that ‘Changhan58’ had greater yield and fructan content in the penultimate internode and higher FEH activity than did ‘Changwu134.’ For ‘Changhan58.’ applying 195 kg·ha?1(120 +75) N plus 45000 kg·ha?1 of pig manure and 120 kg·ha?1 phosphate under conservation tillage produced the highest yield (6769 ka/ha), fructan content in penultimate internode, water use efficiency (WUE), as well as FEH activity among the fertilizer and tillage treatments. Therefore, routine soil management for wheat should focus on combined use of manures and inorganic fertilizer to enhance the amount and transportation efficiency of WSC and ultimately ensure greater yield. 相似文献
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Min-Jin Kwak Min-Young Park Yong-Soon Choi Junghwan Cho Duleepa Pathiraja Jonggun Kim Hanbae Lee In-Geol Choi Kwang-Youn Whang 《畜牧与生物技术杂志(英文版)》2022,(1):256-264
Background: Gut is a crucial organ for the host’s defense system due to its filtering action of the intestinal membrane from hazardous foreign substances. One strategy to strengthen the gut epithelial barrier function is to upregulate beneficial microflora populations and their metabolites. Sophorolipid(SPL), which is a glycolipid biosurfactant, could increase beneficial microflora and decrease pathogenic bacteria in the gastrointestinal tract.Therefore, herein, we conducted an experiment with b... 相似文献
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Na-Hyun Kim Thomas Ciesielski Jung H. Kim Ji-Young Yhee Keum-Soon Im Hae-Mi Nam Il-Hwan Kim Jong-Hyuk Kim Jung-Hyang Sur 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(5):559-564
This report describes a primary central nervous system B-cell lymphoma in a 3-year-old intact female Maltese dog. Canine primary central nervous system lymphomas constitute about 4% of all intracranial primary neoplasms, but comprehensive histopathologic classifications have rarely been carried out. This is the first report of this disease in a young adult dog. 相似文献