全文获取类型
收费全文 | 1578篇 |
免费 | 73篇 |
国内免费 | 6篇 |
专业分类
林业 | 74篇 |
农学 | 67篇 |
基础科学 | 2篇 |
192篇 | |
综合类 | 72篇 |
农作物 | 52篇 |
水产渔业 | 109篇 |
畜牧兽医 | 976篇 |
园艺 | 12篇 |
植物保护 | 101篇 |
出版年
2022年 | 13篇 |
2021年 | 36篇 |
2020年 | 24篇 |
2019年 | 26篇 |
2018年 | 29篇 |
2017年 | 40篇 |
2016年 | 42篇 |
2015年 | 49篇 |
2014年 | 57篇 |
2013年 | 111篇 |
2012年 | 100篇 |
2011年 | 99篇 |
2010年 | 35篇 |
2009年 | 48篇 |
2008年 | 69篇 |
2007年 | 76篇 |
2006年 | 94篇 |
2005年 | 94篇 |
2004年 | 92篇 |
2003年 | 91篇 |
2002年 | 58篇 |
2001年 | 49篇 |
2000年 | 52篇 |
1999年 | 42篇 |
1998年 | 5篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 14篇 |
1991年 | 15篇 |
1990年 | 17篇 |
1989年 | 15篇 |
1988年 | 16篇 |
1987年 | 18篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1979年 | 9篇 |
1977年 | 7篇 |
1974年 | 5篇 |
1972年 | 7篇 |
1970年 | 3篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1965年 | 3篇 |
排序方式: 共有1657条查询结果,搜索用时 0 毫秒
1.
ABSTRACT: To clarify the quantitative changes in the transport of orally intubated protein into the blood circulation as macromolecules in development, immunoglobulin Y (IgY) extracted from chicken eggs was administered orally to juvenile Japanese eel, Anguilla japonica . For the first experiment, which was performed before the commencement of artificial feeding, the oral delivery of 2.0 μg/0.1 g bodyweight of IgY resulted in a rapid increase in plasma IgY to a maximum of 2.30 μg/mL. However, the transport of IgY into the blood decreased significantly in the experiments that followed, which were performed after 12, 25 and 42 days. During this period, bodyweight increased approximately by a factor of eight, and rapid growth of the stomach was observed histologically. Possible contributions for the development of the alimentary canal to the diminishment of intestinal protein assimilation are discussed. 相似文献
2.
F Urayama T Sato H Shibuya W Shirai M Matsutani R Yamazaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1249-1252
An apocrine adenocarcinoma was observed in the subcutis of the abdomen of golden hamster. Histologically, the tumor cells irregularly formed multiple layers of cysts and some detached cells were presented in the cystic space. PAS stain with alpha-amylase digestion revealed PAS-positive alpha-amylase-resistant granules in the cytoplasm. Immunohistochemically, cytokeratin was demonstrated in the tumor cells. By electron microscopy, the tumor cells had an oval nucleus with invagination, abundant cytoplasmic organelles and microvilli protruding into the intercellular spaces. 相似文献
3.
4.
5.
Ken R. Ito Tomonori Sato Hiro Goto Katsuyoshi Sato Jun Watanabe Masaki Yokoo 《The Journal of Poultry Science》2022,59(3):247
Increasing food loss and waste (FLW) is a global problem, and efforts are being made to use waste food as potential livestock feed material. The amount of self-supplied feed is lower in Japan than in other countries, and the government recommends FLW use for animal feed. Sake (Japanese rice wine) is a traditional alcoholic beverage. During the sake manufacturing process, large amounts of squeezed solids or “lees” (sake lees) are generated. Sake lees are nutritious and functional, but are prone to spoilage. In this study, we investigated whether sake lees should be mixed with animal feed immediately or after drying. To assess the usefulness of sake lees as a poultry feed ingredient and determine the effect of sake lees on intestinal immunity, we performed a feeding trial with three treatments: a raw sake lees (RSL) diet, dried sake lees (DSL) diet, and control diet. Three-week-old broilers were fed these diets (n=8 per group) for two weeks. We then calculated feed efficiency and performed RT-qPCR to assess the effects of diet on intestinal immunity. The growth performance in the RSL diet group was equivalent to that in the control diet group. The DSL diet became difficult for broilers to eat, resulting in decreased growth performance. In the ileum of RSL-diet broilers, the mRNA expression levels of TGF-β1 and avian β-defensin (AvBD)12 were significantly increased compared to those of control diet broilers (p<0.05), and a significant correlation was observed between the two genes (p<0.05). Our results indicated that sake lees should not be dried and should be mixed immediately with feed, and this sake lees when fed to chicken activates the intestinal immunity. However, sake lees have a lower fat content than corn, and it is thus important to combine sake lees with high-energy feed. 相似文献
6.
Maeda S Ohno K Nakamura K Uchida K Nakashima K Fukushima K Nakajima M Goto-Koshino Y Fujino Y Tsujimoto H 《Veterinary immunology and immunopathology》2012,148(3-4):226-235
The interaction between fractalkine/CX(3)CL1 and its receptor CX(3)CR1 has been reported to play an important role in various human inflammatory diseases, including inflammatory bowel disease (IBD) mediated by lymphocyte chemoattraction. The objective of this study was to investigate the role of fractalkine and CX(3)CR1 in lymphocyte migration in canine IBD. IBD was diagnosed in 34 dogs, and 19 healthy beagles were used as normal controls. We quantified intestinal mRNA and protein expression of fractalkine and CX(3)CR1 by real-time RT-PCR and ELISA, respectively, and examined the localization of fractalkine in canine intestine by immunohistochemistry. The expression of CX(3)CR1 and surface antigens on peripheral blood mononuclear cells (PBMCs) and intraepithelial lymphocytes (IELs) was analyzed by flow cytometry. Intestinal fractalkine and CX(3)CR1 mRNA was significantly up-regulated in IBD dogs compared with the healthy control dogs. In addition, fractalkine expression on intestinal epithelial cells was significantly increased in the intestinal mucosa of IBD dogs compared with the healthy dogs. CX(3)CR1(+) PBMCs were significantly elevated in IBD dogs and positively correlated with the histopathological severity of IELs infiltration. These CX(3)CR1(+) PBMCs predominantly expressed markers for cytotoxic T cells. Almost all IELs expressed CD3, and the majority of cells expressed CD8 rather than CD4, which was analogous to the CX(3)CR1(+) PBMCs. These results suggest that the fractalkine-CX(3)CR1 interaction may contribute to the pathogenesis of canine IBD through migration of IELs. 相似文献
7.
Tsukamoto A Ohno K Irie M Ohmi A Maeda S Nakashima K Fukushima K Fujino Y Uchida K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):503-506
Endoscopic polypectomy and argon plasma coagulation (APC) were performed in a refractory case of inflammatory colorectal polyps in a 7-year-old male Miniature Dachshund. Colonoscopic examination revealed a large sessile polyp and multiple diffuse small polyps, localized to the descending colon and rectum. The case showed a poor therapeutic response to prednisolone and cyclosporine. Under anesthesia, piecemeal resections were performed by polypectomy. APC was carried out to cauterize the polyp remnants. After treatment, reduction of the lesions and the improvement in clinical signs were observed, without recurrence of lesions for at least 10 months. Endoscopic treatment by polypectomy and APC is suggested to be a therapeutic option for refractory cases of inflammatory colorectal polyps in dogs. 相似文献
8.
Dhakal IP Dhakal P Koshihara T Nagahata H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1241-1245
A total of 355 Murrah cross buffaloes, consisting of 23 subclinical and 332 clinical mastitis cases brought to the Veterinary Teaching Hospital, Chitwan, Nepal from 2002 to 2005, were analyzed to determine the organisms involved, the seasonal occurrence of mastitis, and antibiotic susceptibility of mastitis pathogens. Coagulase negative Staphylococci (CNS) such as Staphylococcus albus and S. epidermidis were the predominant organisms associated with subclinical cases, and CNS and Coliforms in clinical cases. The maximum number (16%) of clinical cases of mastitis were observed in the month of July, when temperature and humidity are highest. The incidence of clinical mastitis was higher in animals during 1st calving and during the 1st month of parturition. Resistance to antibiotics was determined for 55, 23 and 149 isolates of Staphylococcus spp., Streptococcus spp. and Coliforms, respectively. In vitro drug sensitivity testing revealed that enrofloxacin had the highest average sensitivity (91%) for all types of bacteria. The effectiveness of other drugs detected were gentamicin (87%), tetracycline (83%) and chloramphenicol (82%). The antibiogram showed that both gentamicin and enrofloxacin are slowly becoming resistant. Mastitis pathogens have developed resistance to ampicillin and penicillin. 相似文献
9.
Kobayashi Y Inoue N Sato G Itou T Santos HP Brito CJ Gomes AA Santos MF Silva MV Mota CS Ito FH Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(7):691-696
The incidence of canine rabies has been widely reported in Brazil, and new rabies virus (RV) variants, genetically similar to canine RV, have recently been isolated from foxes. In order to derive the epidemiological characteristics of Brazilian Carnivora RV, Brazilian RVs isolated from dogs, cats, and foxes were genetically analyzed. Brazilian Carnivora RV isolates were divided into 2 main lineages. The predominant lineage was found in dogs and cats, which included the Argentinean and Bolivian Carnivora RV isolates, and was extensively distributed throughout Brazil and surrounding countries. The other lineage consisted of three sublineages containing Brazilian dog and fox RV isolates, with the dog sublineages located on an internal branch of 2 fox sublineages, suggesting that RV transmission events might have occurred between foxes and dogs in the past. These results suggest that contact between dogs and wildlife has the potential to generate new rabies variants and that it is important to control RV infection cycles in both dogs and wildlife to prevent spread of rabies infection. 相似文献
10.
Performance of a closed recirculating system with foam separation, nitrification and denitrification units for intensive culture of eel: towards zero emission 总被引:10,自引:0,他引:10
Yoshihiro Suzuki Toshiroh Maruyama Hiroyuki Numata Hajime Sato Makio Asakawa 《Aquacultural Engineering》2003,29(3-4):165-182
The development of a closed recirculating aquaculture system that does not discharge effluents would reduce a large amount of pollutant load on aquatic bodies. In this study, eel were reared in a closed recirculating system, which consisted of a rearing tank, a foam separation unit, a nitrification unit and a denitrification unit. The foam separation unit has an inhalation-type aerator and supplies air bubbles to the rearing water. The growth of eel, which were fed a commercial diet, was satisfactory, with gross weight increases of up three times in 3 months. The survival rate under the congested experimental conditions was 91%. The foam separation unit maintained oxygen saturation in the rearing water at about 80%. Furthermore, fine colloidal substances were absorbed on the stable foam formed from eel mucus and were removed from the rearing water by foam separation. Ammonia oxidation and the removal of suspended solids were accomplished rapidly and simultaneously in the nitrification unit. The ammonia concentration and turbidity were kept at less than 1.2 mg of N per litre and 2.5 units, respectively. When the denitrification process was operated, nitrate that accumulated in the rearing water (151 mg of N per litre) was reduced to 40 mg of N per litre. The sludge was easily recovered from the nitrification and denitrification tanks, and the components were found suitable as compost. Based on these results, the intensive aquaculture of freshwater fish such as eel can be achieved using a closed recirculating system without emission. 相似文献