Hypocholesterolemic effects of phenolic extracts and purified hydroxytyrosol recovered from olive mill wastewater in rats fed a cholesterol-rich diet

In our previous studies, a phenolic-rich extract of olive mill wastewaters (OMW) was prepared under optimal conditions, using a continuous countercurrent extraction unit, and hydroxytyrosol was purified from the obtained OMW extract. The antioxidant activity of OMW extract and hydroxytyrosol was determined by a series of models in vitro. In this study, the hypocholesterolemic effects of hydroxytyrosol and OMW extract in rats fed a cholesterol-rich diet were tested. Wistar rats, fed a standard laboratory diet or a cholesterol-rich diet for 16 weeks, were used. Serum lipid levels, as well as thiobarbituric acid reactive substances (TBARS) and superoxide dismutase and catalase activities in liver were examined. Cholesterol-rich diet-induced hypercholesterolemia was manifested in the elevation of serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C). Administration of a low-dose (2.5 mg/kg of body weight) of hydroxytyrosol and a high-dose (10 mg/kg of body weight) of OMW extract significantly lowered the serum levels of TC and LDL-C while increasing the serum levels of high-density lipoprotein cholesterol (HDL-C). Furthermore, the TBARS contents in liver, heart, kidney, and aorta decreased significantly after oral administration of hydroxytyrosol and OMW extract as compared with those of rats fed a cholesterol-rich diet. In addition, OMW phenolics increased CAT and SOD activities in liver. These results suggested that the hypocholesterolemic effect of hydroxytyrosol and OMW extract might be due to their abilities to lower serum TC and LDL-C levels as well as slowing the lipid peroxidation process and enhancing antioxidant enzyme activity.     

Impregnation of ethylcellulose microcapsules containing jojoba oil onto compressive knits developed for high burns

The medical uses of textile fibers and fabric forms keep on growing. Today, the knits can be used in the medical field. The aim of this present work was to prepare compressive knits, impregnated by ethylcellulose (EC) microcapsules, for the burnt skin. Ethylcellulose microcapsules containing jojoba oil were prepared via the phase separation method. Jojoba oil was chosen because it plays an essential role in skin hydration and avoids the sebum accumulation. Ethylcellulose microcapsules obtained were grafted on two different compressive knit surfaces by impregnation, using the Acrylic Resin (AR) as binder. The prepared microcapsules were analyzed by Scanning Electron Microscopy (SEM). The stability of the microcapsules, the efficiency of the microencapsulation method and the jojoba oil content determination were also investigated. This method of application improved the fabric surface and permitted in maximum the preserving of initial characteristics of knit, such as touch, flexibility and lightness.