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AIM:To explore the relationship between the expression of transketolase-like gene 1 (TKTL1) and glycolysis metabolism in human cervical cells. METHODS:The changes of hypoxia-inducible factor 1α (HIF-1α) expression and the activity of glycolytic key enzymes, hexokinase Ⅱ (HK-Ⅱ) and lactate dehydrogenase (LDH), under hypoxia in human cervical cell line HeLa were observed after TKTL1 was knockdown by siRNA. The specific siRNA expression vector targeting TKTL1 gene was constructed, and the recombinant plasmid was transfected into HeLa cells. The effects of TKTL1 silencing were evaluated by detecting transketolase (TKT) activity and TKTL1 mRNA expression using RT-PCR. The changes of HIF-1α expression and HK-Ⅱ expression, and HK-Ⅱ and LDH activity were also observed in transfected HeLa cells. RESULTS:The mRNA expression of TKTL1 and the activity of TKT decreased significantly (P<0.01) after TKTL1 silencing. Meanwhile, all HIF-1α expression, HK-Ⅱexpression, and HK-Ⅱ and LDH activity decreased significantly compared with the untransfected cells (P<0.01). CONCLUSION:Silencing of TKTL1 gene in human cervical cancer cells by siRNA down-regulates HIF-1α expression and the activity of glycolytic key enzymes, thus changing the malignant phenotype of carcinoma cells. 相似文献
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积极发掘新的玉米矮秆资源并进行研究和利用,有利于玉米矮化育种。对比研究了矮秆突变体K125d和同源自交系K211之间的形态差异,通过与7个不同株高自交系配制正反交F1,回交B1、B2和自交F2群体,分析突变体K125d矮秆性状的遗传模式,其矮秆基因定位用BSA-SSR标记法。结果表明,与K211相比,K125d的生育期极显著增长,株高极显著降低;叶片重叠密集,叶数和叶宽极显著增加,叶片夹角极显著降低。所有群体在2个不同生态点试验结果一致,正反交F1均为高秆;7个B1群体和F2群体株高分离比例分别符合1∶1和3∶1;除K123d外,6个B2群体均为高秆,表明突变体K125d的株高由1对隐性细胞核基因控制,暂命名为d125。以(K125d×K236)F2为定位群体,将基因d125定位于1号染色体SSR标记umc2569和umc1278之间,其距离为6.6,5.1 cM。以br2基因模型GRMZM... 相似文献
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[目的]明确在四川成都和西昌生产玉米品种正红431种子时适宜的收获期,为生产高质量种子提供技术支撑.[方法]通过2个基地不同成熟度正红431种子丙二醛(MDA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性和淀粉、蛋白质、脂肪的含量以及种子发芽率的变化来综合判断.[结果]成都基地正红431种子MDA含量和POD... 相似文献
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为明确28个玉米品种苗期的耐重金属性和子粒对重金属的吸收特性,本研究通过测定重金属Cd、Pb、Cr、Zn、Cu单一胁迫对玉米幼苗株高等11个生理生化指标的影响,评价玉米幼苗对重金属的耐受性。通过测定不同玉米品种在四川省3个生态区的子粒重金属含量,评价子粒的重金属吸附特性。结果表明,根据苗期重金属耐受性可将28个玉米品种分为4类,其中瑞玉612和正红412为强耐重金属品种,正红818等13个品种为耐重金属品种,正红510等5个品种为中等耐重金属品种,中单808等8个品种为重金属敏感型品种。根据子粒对重金属的吸附特性可分为4类,正红205等12个品种子粒重金属含量最低,正红510等3个品种子粒重金属含量最高,瑞玉612等13个品种重金属含量处于中等水平。综合以上结果,正红818等13个品种的苗期重金属耐受性强,且子粒对重金属的吸附低,表明这些品种具有较高的推广应用安全性。 相似文献
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