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Effect of farm characteristics and practices on hygienic quality of ovine raw milk used for artisan cheese production in central Italy 下载免费PDF全文
Elisa Carloni Annalisa Petruzzelli Giulia Amagliani Giorgio Brandi Francesco Caverni Piermario Mangili Franco Tonucci 《Animal Science Journal》2016,87(4):591-599
A survey on ovine dairy farms directly transforming own‐produced milk, in the Italian Marche region, was carried out to assess flock and milking practices that may influence milk hygienic‐sanitary conditions. A census survey established that 24 dairy farms were located in this region. Bulk milk samples were collected throughout the milking period in each dairy farm in 2013. Analyzed variables were: (i) chemical parameters such as fat, protein and lactose content, dry matter and pH; and (ii) total bacterial (TBC) and somatic cell counts (SCC). Chemical parameter values were in agreement with published data while, geometric mean (GM) log10 SCC was 5.91 and TBC GM was 57 978 colony forming units/mL, in compliance with Eropean Union criteria. A positive correlation was found between SCC and TBC when GMs of all farm data were considered (Spearman's rho = 0.7925; P = 0.0001). Statistical analysis did not show significant correlation between SCC or TBC GM and dairy farm principal characteristics. Although SCC levels detected in the present study should suggest the need to implement mastitis control programs, Marche's dairy sheep flocks revealed a good hygienic condition level. This is an important aspect in implementing safety for end users of the final product. 相似文献
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Giorgio Gambino Jeannette Bondaz Ivana Gribaudo 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(4):397-404
The distribution of some grapevine viruses in flower explants, embryogenic and non-embryogenic calli, single somatic embryos
and plants regenerated from embryogenic cultures was investigated by RT-PCR and ELISA. Immature anthers and ovaries of the
cultivars Grignolino infected by GRSPaV, GLRaV-1 and GVA, Müller-Thurgau infected by GRSPaV and GLRaV-3 and Bosco infected
by GRSPaV were cultivated on media inducing indirect somatic embryogenesis. Viruses were detected both in anthers and ovaries.
Four months after culture initiation 65.6% of tested calli were infected by at least one virus; high percentages of virus
infection were found in calli originating from ovaries. No virus was detected in calli tested 8 months after culture initiation,
as well as in single somatic embryos or in embryo-derived plantlets. Somatic embryogenesis confirmed its effectiveness in
eliminating phloem-limited grapevine viruses. Regeneration of RT-PCR negative plantlets occurred even when at least a sector
of the callus was still infected: the mechanism whereby somatic embryos are freed of some viruses could be related to the
rapid proliferation of embryogenic cells within the callus or to the origin of the embryogenic callus from virus-free cells
within the original explant. 相似文献
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Agostino Sorgonà Maria Rosa AbenavoliPietro Giorgio Gringeri Giovanni Cacco 《Scientia Horticulturae》2006
Nitrogen use efficiency (NUE) definitions, commonly used in literature, are evaluated in response to nitrate availability in four citrus rootstocks, Rough Lemon (Citrus jambhiri Lush) (RL), Sweet Orange (Citrus sinensis (L.) Osbeck) (SwO), Cleopatra Mandarin (Citrus reshni Hort ex Tan.) (CM) and Sour Orange (Citrus aurantium L.) (SO). The application of diverse definitions determine different characterizations in N-efficiency among rootstocks. Nitrogen utilization efficiency (NUtE) and nitrogen efficiency ratio (NER) determine equal level of nitrogen efficiency among all rootstocks. Total nitrogen accumulation (TNA), nitrogen uptake efficiency (NUpE), shoot dry weight (SDW) and total leaf area (TLA) response curves produce the same NUE characterization: SO and SwO were nitrate use efficient and inefficient rootstocks, respectively, while the RL and CM exhibit superior and inferior genetic potential, respectively. 相似文献
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Cautela D Castaldo D Santelli F Laratta B Pirrello AG Malorni A Boscaino F 《Journal of agricultural and food chemistry》2007,55(4):1627-1637
Investigation of PCDDs, PCDFs, PCBs, Al, As, Pb, Ba, Co, Cu, Cr, Fe, Mn, Cd, Ag, Sn, Zn, and Hg contents in 60 samples of cold-pressed essential oils produced in Calabria and Sicily in 2003-2005 was carried out. PCDDs, PCDFs, and PCBs were analyzed by HRGC-HRMS techniques using U.S. EPA 1613/94 and U.S. EPA 1668/A (1999) analytical methods. Mineral components were determined through GFAAS techniques; Hg content was determined by FI-M/H-AAS. The results of this study showed that essential oil contamination was due to a widespread pollution, typical background of rural areas, with relatively higher concentrations of PCDDs compared to PCDFs and little presence of PeCDF. Congeners OCDD, HpCDF, and OCDF were found at high concentrations. Regarding mineral components, mean values of Cr, Fe, and Ni were in agreement with data reported in the literature. Concentrations of As and Pb were below the maximum limits accepted by the current legislation. Finally, none of the samples analyzed were contaminated with Hg. 相似文献