Induction of Immunogenic Response in BALB/c Mice by Live and Killed Form of Recombinant Lactococcus lactis Displaying EG95 of Echinococcus granulosus

Background:CE is a zoonotic parasitic infection caused by Echinococcus granulosus worldwide and is associated with economic losses among livestock animals. EG95 is an immunogenic antigen from the E. granulosus. Lactococcus lactis has been prested as a safe vehicle for antigen delivery. The goal of this study was to design a novel L. lactis strain displaying EG95 as a vaccine delivery system. Methods:The eg95 encoding gene fragment fused to the M6 anchoring protein was cloned into the pNZ7021 vector, and L. lactis NZ9000 displaying recombinant EG95 was constructed. The expression of an approximately 32-kDa EG95 protein was confirmed by Western blotting and immunofluorescence analysis. The immune responses were evaluated in BALB/c mice immunized orally and subcutaneously with the live and killed recombinant L. lactis, respectively. Results:Total IgG level in mice immunized with heat-killed recombinant L. lactis (pNZ7021-eg95) significantly increased compared to the control group. sIgA was significantly higher in mice received live recombinant L. lactis (pNZ7021-eg95) compared to the control mice. Splenic lymphocytes from immunized mice represented the high levels of IFN-γ and the low-levels of IL-4 and IL-10. Conclusion:Our results indicate that immunization with EG95-expressing L. lactis can induce both specific humoral and cellular immune responses in mice. Key Words: Echinococcus granulosus, Lactococcus lactis, Immunization, Vaccines     

Soil physical properties degrade further on skid trails in the year following operations

Short-term recovery of soil physical properties on skid trails was investigated upon cessation of skidding operations and 1 year later. Bulk density and porosity were assessed at three levels of traffic intensity and two slope gradients. Compared to undisturbed areas, bulk density increased, total porosity and macroporosity decreased, and microporosity increased in the compacted areas immediately after skidding. In all cases, changes were significantly greater when traffic intensity was higher and when slopes were steeper. Surface soil compaction did not show any recovery over the 1-year period, illustrating the persistent effects of compaction on soil structure. In fact, surficial compaction further increased and macroporosity further decreased after 1 year of recovery, compared to immediately after skidding. While these changes may reflect inherent small-scale site variability or differences in soil moisture content at times of sampling, direct negative physical impacts on newly exposed soil (such as by raindrops), in addition to the loss of organic matter after canopy removal and skidding, likely delayed any physical recovery, particularly on heavily trafficked trails on steeper slopes. These results raise serious concerns about long-term resilience to traffic on forest soils and indicate the necessity to properly retire skid trails to avoid further soil degradation as trails age.     

Mild salinity improves sugar beet (Beta vulgaris L.) quality

Abstract

Sugar beet (Beta vulgaris L.) is cultivated mainly on saline soils. Low levels of salinity stimulate growth of this crop plant possibly due to production of broader leaves as sources of assimilates. In this work, six cultivars were studied under mild salinity (EC = 5.5 dS m^?1) in a field experiment to analyse its effect on growth parameters and yield of storage root and sugar accumulation. An attempt was also made to determine the contributing role of photosynthetic gas exchange in response of sugar beet plants to salinity. Production of greater leaf area in salinized plants occurred only transitionally in the early growth period; in progression of the growing season it was decreased, and at 3rd harvest (100 days after treatment) it was significantly lower compared with control plants without cultivar differences. Leaf chlorophyll fluorescence, net photosynthesis rate, and stomatal conductance did not change by salinity significantly. Although, at the end of growth season, leaf area and potential photosynthesizing component of salinized plants on the basis of leaf area (LAR) or weight (LWR) were significantly lower than for control plants, weight of storage root and sugar content were up to 90 and 37% higher than in control plants, respectively. Consequently, a considerable higher yield under mild salinity conditions in sugar beet is not attributable to higher leaf area or, therefore, higher photosynthetic capacity of whole plants. Indeed, the storage roots benefit from lower dry matter and surface production of shoot during the late growing season (because of lower nitrogen assimilation and a slight drought stress of salinized plants) and a change in dry-matter partitioning in favor of roots takes place. However, a possible special effect of Na on carbon allocation for storage and structure and involvement of growth regulators in the change of root-shoot allometry could not be excluded.     

The impact of land afforestation on carbon stocks surrounding Tehran,Iran

The city of Tehran,like many polluted metropolises of the world,has higher emissions of greenhouse gases than other cities in Iran,due to heavy consumption of fossil fuel and landuse changes.To estimate carbon sequestration in two 40 year-old stands of planted Cupressus arizonica and Fraxinus rotundifolia in degraded lands surrounding Tehran,sampling of above-and below-ground biomass,soil(at two depths of 0-15 and 15-30 cm),and leaf litter was done by systematic random sampling.The total carbon stocks of C.arizonica and F.rotundifolia stands were respectively 328.20 and 150.69 Mg·ha-1.The aboveground biomass with 233.16(71%)Mg·ha-1 in C.arizonica and88.16(58.50%)Mg·ha-1 in F.rotundifolia contributed the most shares to carbon sequestration.The diameter at breast height,total height,basal area,total volume,and biomass of C.arizonica were significantly(p0.01)higher than those of F.rotundifolia.Also the depth of 0-30 cm of soil contributed between 18.29%and 32.15%of total ecosystem carbon,respectively.The economic value of carbon sequestration in the two stands in 2011 was calculated at 3.5 and 2.5 million dollars,respectively.Our results indicate that afforestation of the degraded land surrounding Tehran would sequester more carbon than would continuously degraded land,the current status quo.These stands can absorb atmospheric CO2 at different rates,thus tree species selection and stand development should be considered in planning future afforestation projects.     

Effects of Wheeled Cable Skidding on Residual Trees in Selective Logging in Caspian Forests

There are increasing demands on silvicultural practices to reduce the adverse impacts of harvesting. Damage to residual trees caused by ground-based skidding was assessed in stands with low [3.4 stems per hectare (sph), 14.36 m³/ha], medium (5.2 sph, 21.61 m³/ha), and high (7.1 sph, 25.95 m³/ha) harvest intensities. Skidding was conducted using a cable skidder. After the skidding operation, a field survey was conducted to collect data on all residual trees (species, dbh, height) and on tree wounds (size class, location, intensity of damage). It was hypothesized that increased harvest intensity leads to increased injury rates. The treatment with the highest harvest intensity was found to cause the highest percentage of damage and the largest stem wounds. The most common types of damage were stem wounds to the cambium layer and bark scrapes. In all three harvest intensity treatments the probability of individual tree damage decreased as skid-trail cross slope decreased and distance to skid-trail edge increased. It is concluded that harvesting intensity should be limited to 5 trees/ha during each harvesting operation to reduce extent of tree damage and thus future financial loss.     

Deforestation effects on soil quality and water retention curve parameters in eastern Ardabil,Iran

The land use change from natural to managed ecosystems causes serious soil degradation. The main objective of this research was to assess deforestation effects on soil physical quality attributes and soil water retention curve (SWRC) parameters in the Fandoghlou region of Ardabil province, Iran. Totally 36 surface and subsurface soil samples were taken and soil water contents measured at 13 suctions. Alfa (α) and n parameters in van Genuchten (1980) model were estimated by fitting SWRC data by using RETC software. The slope of SWRC at inflection point (S_P) was calculated by Dexter (2004) equation. The results indicated that with changing land use from forest (F) to range land (R) and cultivated land (C), and also with increasing soil depth from 0–25 to 75–100 cm in each land use, organic carbon, micropores, saturated and available water contents decreased and macropores and bulk density increased significantly (P < 0.05). The position of SWRC shape in F was higher than R and C lands at all soil depths. Changing F to R and C lands and also increasing soil depth in each land use significantly (P < 0.05) increased α and decreased n and S_P. The average values of S_P were obtained 0.093, 0.051 and 0.031 for F, R and C, respectively. As a result, deforestation reduced soil physical quality by affecting SWRC parameters.     

Economic analysis on log damage during logging operation in Caspian Forests

Waste wood was studied in an economic enterprise by logging, function, tree species and log size in four Caspian hardwood sites. Damaged logs were recorded with additional information obtained for the location, dimensions and type of damage. The data were analyzed statistically to determine significant differences of damage during logging process. The results indicated that animal harvesting systems cause more volume (40.5% of log volume) and value loss (89.5 $·m^?3) to logs than mechanized harvesting systems (13.9% and 6.0 $·m^?3), also bucking resulted in significantly more volume (9.9% of log volume) and value loss (5.5 $·m^?3) when compared to skidding (0.2% of log volume and 0.2 $·m^?3), decking (0.4% of log volume and 0.2 $·m^?3) and loading (0.2% of log’s volume and 0.3 $·m^?3) operations. Study showed that the processes of skidding, decking and loading of logs have very little impact on damage levels. Volume and value losses of damaged logs are not sensitive to tree species and log size. The information from the field study is important in creating new guidelines or training to help minimize hardwood log damage during the timber harvesting process.     

Effect of selective cutting type on the chainsaw productivity in Caspian Forests

Time and motion studies were conducted to evaluate the impact of selective cutting type on Chainsaw productivity in Caspian forests,the selective cutting performed as single and group selection cutting in the region.The selective cutting was performed on a 42-ha tract with an average slope of 30%.The results indicate that felling time for per tree is most affected by diameter at breast height,the distance among harvested trees in single-tree selection method and diameter at breast height in group selection method.The production rate in single and group selection cutting were 21.2 m 3 h-1 and 28.4 m 3 h-1 for one person,respectively.Considering the gross and net production rate in single and group selection cutting,the unit cost was 1.11 USD m-3 and 0.88 USD m-3,respectively.The results indicate that group selection cutting can be more profitable than single-tree selection method.     

Potential Antiviral Agents from Marine Fungi: An Overview

Biodiversity of the marine world is only partially subjected to detailed scientific scrutiny in comparison to terrestrial life. Life in the marine world depends heavily on marine fungi scavenging the oceans of lifeless plants and animals and entering them into the nutrient cycle by. Approximately 150 to 200 new compounds, including alkaloids, sesquiterpenes, polyketides, and aromatic compounds, are identified from marine fungi annually. In recent years, numerous investigations demonstrated the tremendous potential of marine fungi as a promising source to develop new antivirals against different important viruses, including herpes simplex viruses, the human immunodeficiency virus, and the influenza virus. Various genera of marine fungi such as Aspergillus, Penicillium, Cladosporium, and Fusarium were subjected to compound isolation and antiviral studies, which led to an illustration of the strong antiviral activity of a variety of marine fungi-derived compounds. The present review strives to summarize all available knowledge on active compounds isolated from marine fungi with antiviral activity.