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排序方式: 共有81条查询结果,搜索用时 15 毫秒
1.
Jin-Kyu Rhee Ok Kyu Park Aeju Lee Dae Hyeok Yang Kyeongsoon Park 《Marine drugs》2014,12(12):6038-6057
Theranostics is an integrated nanosystem that combines therapeutics with diagnostics in attempt to develop new personalized treatments with enhanced therapeutic efficacy and safety. As a promising therapeutic paradigm with cutting-edge technologies, theranostic agents are able to simultaneously deliver therapeutic drugs and diagnostic imaging agents and also monitor the response to therapy. Polymeric nanosystems have been intensively explored for biomedical applications to diagnose and treat various cancers. In recent years, glycol chitosan-based nanoagents have been developed as dual-purpose materials for simultaneous diagnosis and therapy. They have shown great potential in cancer therapies, such as chemotherapeutics and nucleic acid and photodynamic therapies. In this review, we summarize the recent progress and potential applications of glycol chitosan-based fluorescent theranostic nanoagents for cancer treatments and discuss their possible underlying mechanisms. 相似文献
2.
Evaluation and characterization of a novel probiotic Lactobacillus pentosus PL11 isolated from Japanese eel (Anguilla japonica) for its use in aquaculture 下载免费PDF全文
S.‐J. Lee M.‐A. Hossain S. Belew J.‐Y. Kim M.‐H. Rhee J.‐C. Kim S.‐C. Park 《Aquaculture Nutrition》2015,21(4):444-456
In the present study, a potential Lactobacilli probiotics were isolated from Japanese eels (Anguilla japonica) and characterized and evaluated for their possible use in eel farming. Sixteen Lactobacilli were isolated from intestines of Japanese eels, using selective media. The lactobacilli strains (represented as PL1 to PL16) were screened by their ability to produce digestive enzyme. Among these, three strains (PL11, PL13 and PL16) producing four digestive enzymes (amylase, cellulase, protease and phytase) simultaneously were characterized further using API ZYM kit. From these, PL11 (Lactobacillu (L.) pentosus) was identified as potential probiotics candidate producing 15 enzymes among 20 tested. Further examination of biological activities of PL11 revealed tolerance against pH, artificial bile juice and antibacterial activity against several fish pathogenic bacteria. The in vitro competitive exclusion assay also revealed 88.4% reduction in adhesion of fish pathogen (Edwardsiella tarda) by PL11 to host intestinal mucus. In vitro incubation of Japanese eel foregut with Baclight‐labelled PL11 showed colonization of the enterocyte surface by confocal and scanning electron microscopy. In summary, PL11 isolated from eels could serve as a potential probiotics with acid and bile tolerance, production of digestive enzymes, antibacterial activity and inhibition of fish pathogen adhesion to intestinal mucus. 相似文献
3.
El-Hassan A El-Sayed M Hamed AI Rhee IK Ahmed AA Zeller KP Verpoorte R 《Fitoterapia》2003,74(1-2):184-187
The aerial part of Leptadenia arborea has been shown to contain pinoresinol (1), syringaresinol (2), leucanthemitol (3) and E-ferulaldehyde (4). These known compounds are being reported for the first time from this plant. Among them, syringaresinol has shown an inhibitory effect against acetylcholinesterase. The IC(50) (the concentration of 50% enzyme inhibition) value of this compound was 200 microg/ml. 相似文献
4.
Kim YM Park K Joo GJ Jeong EM Kim JE Rhee IK 《Journal of agricultural and food chemistry》2004,52(13):4192-4196
A gene responsible for the chlorothalonil biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, capable of efficiently dissipating the chlorothalonil. The gene encoding glutathione S-transferase (GST) of O. anthropi SH35B was expressed in Escherichia coli, and the GST was subsequently purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the GST in the presence of glutathione. LC-MS analysis supported the formation of mono-, di-, and triglutathione conjugates of chlorothalonil by the GST. The monoglutathione conjugate was observed as an intermediate in the enzymatic reaction. The triglutathione conjugate has not been previously reported and seems to be the final metabolite in the biotransformation of chlorothalonil. The glutathione-dependent biotransformation of chlorothalonil catalyzed by the bacterial GST is reported. 相似文献
5.
The complete primary structure of protein kinase C--the major phorbol ester receptor 总被引:71,自引:0,他引:71
P J Parker L Coussens N Totty L Rhee S Young E Chen S Stabel M D Waterfield A Ullrich 《Science (New York, N.Y.)》1986,233(4766):853-859
Protein kinase C, the major phorbol ester receptor, was purified from bovine brain and through the use of oligonucleotide probes based on partial amino acid sequence, complementary DNA clones were derived from bovine brain complementary DNA libraries. Thus, the complete amino acid sequence of bovine protein kinase C was determined, revealing a domain structure. At the amino terminal is a cysteine-rich domain with an internal duplication; a putative calcium-binding domain follows, and there is at the carboxyl terminal a domain that shows substantial homology, but not identity, to sequences of other protein kinase. 相似文献
6.
Park HG Kim SH Kwon SH Ju YG Yang JK Baek JH Kim SB Lee YH 《Science (New York, N.Y.)》2004,305(5689):1444-1447
We report the experimental demonstration of an electrically driven, single-mode, low threshold current (approximately 260 microA) photonic band gap laser operating at room temperature. The electrical current pulse is injected through a sub-micrometer-sized semiconductor wire at the center of the mode with minimal degradation of the quality factor. The actual mode of interest operates in a nondegenerate monopole mode, as evidenced through the comparison of the measurement with the computation based on the actual fabricated structural parameters. As a small step toward a thresholdless laser or a single photon source, this wavelength-size photonic crystal laser may be of interest to photonic crystals, cavity quantum electrodynamics, and quantum information communities. 相似文献
7.
Hong IH Bae SH Lee SG Park JK Ji AR Ki MR Han SY Lee EM Kim AY You SY Kim TH Jeong KS 《Veterinary ophthalmology》2011,14(1):61-65
A 4-year-old, neutered female Cocker Spaniel was presented to the veterinary clinic for protrusion of the left third eyelid. When the third eyelids from both eyes were everted, lobulated masses were present on the bulbar surface. The left third eyelid had a larger protrusion. There was no apparent associated ocular or systemic involvement. The tumor of left third eyelid was removed and referred for histological examination. Histologically, there were proliferations of lymphoid follicles surrounded by lymphoid cells forming a marginal zone. Those lymphoid cells occasionally infiltrated into conjunctival epithelium. A few apoptotic bodies with karyopyknotic and karyorrhexic nuclei were observed in the germinal center of lymphoid follicles. Mitotic figures were rare. On immunohistochemistry, tumor cells expressed CD79a but not CD3. A diagnosis of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) of the third eyelid was established based on the histological and immunophenotypical features. At the 1-year follow-up, there was no evidence of recurrence of the mass at the area of excision of the left third eyelid and the remaining tumor of the right third eyelid was still a similar size. The dog still showed no significant findings, except those of the tumor, and no evidence of systemic involvement. To the authors' knowledge, this is the first reported case of MALT lymphoma of the third eyelid in a dog. 相似文献
8.
Park SK Rhee CO Bae DH Hettiarachchy NS 《Journal of agricultural and food chemistry》2001,49(5):2308-2312
Edible films were prepared from solutions of soy protein with calcium salts and glucono-delta-lactone (GDL). Calcium salts cross-linking interactions with soy-protein isolate (SPI) could result in the formation of films with rigid three-dimensional structure. GDL contributed to the formation of a homogeneous film structure due to increased protein--solvent attraction. Tensile strength (TS) of calcium sulfate treated SPI film (8.6 MPa) was higher than the TS of calcium chloride treated SPI films (6.4 MPa) and the control SPI film (5.5 MPa). Puncture strength (PS) of calcium sulfate treated SPI film (9.8 MPa) was higher than the PS of calcium chloride treated SPI films (8.5 MPa) and the control SPI film (5.9 MPa). SPI film formulated with GDL had larger elongation at break (39.4%) than that of SPI control film (18.2%). Calcium salts and GDL-treated SPI films had lower water-vapor permeability than the SPI control film. 相似文献
9.
Jang KH Ryu EJ Park BS Song KB Kang SA Kim CH Uhm TB Park YI Rhee SK 《Journal of agricultural and food chemistry》2003,51(9):2632-2636
A new levan fructotransferase (LFTase) isolated from Arthrobacter oxydans J17-21 was characterized for the production of difructose dianhydride IV (DFA IV). LFTase was purified to apparent homogeneity by Q-Sepharose ion exchange chromatography, Mono-Q HR 5/5 column chromatography, and gel permeation chromatography. The enzyme had an apparent molecular mass of 54000 Da. The optimum pH for the enzyme-catalyzed reaction was pH 6.5, and the optimum temperature was observed at 45 degrees C. The LFTase was activated by the presence of CaCl(2) and EDTA-2Na but inhibited strongly by MnCl(2) and CuSO(4) at 1 mM and completely by FeSO(4) and Ag(2)SO(4) at 1 mM. A bacterial levan from Zymomonas mobilis was incubated with an LFTase; final conversion yield from the levan to DFA IV was 35%. Neither inulin, levanbiose, sucrose, dextran, nor starch was hydrolyzed by LFTase. DFA IV was very stable at acidic pH and high temperature, thus indicating that DFA IV may be suitable for the food industry and related areas. 相似文献
10.
Binod Prasad Luitel Na-Young Ro Ho-Cheol Ko Jung-Sook Sung Ju-Hee Rhee On-Sook Hur 《Journal of Crop Science and Biotechnology》2018,21(5):499-506
A total of 47 core collections of pepper (Capsicum chinense Jacq.) conserved in National Agrobiodiversity Center (NAC) were studied under field condition at Jeonju over two consecutive years (2015 and 2016). All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. Distribution of fruit characters (fruit length, width, and fruit wall thickness) among the accessions was positively skewed. Of the 47 accessions evaluated, 38.3% accessions had conical shaped fruits and mature fruit color was predominantly red (51.1%), orange (21.3%) and yellow (14.9%). Principal component analyses revealed that (i) 56.64% of the qualitative (fruit shape, color and fruit surface) variation and (ii) 89.42% of the quantitative (plant width, height and fruit maturity days) variation were explained by the first two components. Clustering revealed two groups and dendrogram revealed morphological variation among accessions. The phenotypic diversity exists in this core collections provide valuable information to improve agronomic traits in pepper breeding program. 相似文献