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Influence of organic phosphorus on reproductive performance and metabolic profiles of anoestrous Farafra ewes in subtropics at the end of breeding season
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The effect of organic phosphorus on metabolic, haematological and hormonal status, restoration of ovarian functions and conception rate in anoestrous Farafra ewes in subtropics were evaluated. Anoestrous Farafra ewes (n = 24; 34.72 ± 0.52 kg body weight) were allocated into two equal groups: control and phosphorus groups. The ewes of phosphorus group were treated with sodium 4‐dimethylamino‐2‐methyl‐phenyl‐phosphonate as an organic bound phosphorous twice a week for successive 3 weeks. Ovarian follicle development and corpora lutea were checked three times a week till occurrence of oestrus using ultrasonography while pregnancy was confirmed at 30 days post‐service. Plasma metabolites, reproductive hormones, thyroid hormones and minerals were detected at weeks ?2, ?1, 0 (mating day) and + 4 weeks post‐oestrus. Phosphorus group had significantly (p < .05) short interval to oestrous resumption if compared to control ewes (2.1 ± 0.8 weeks vs . 4.6 ± 1.1 weeks). In addition, phosphorous supplementation significantly (p < .05) increased the number of antral follicles (developed and their sizes in addition to sizes of corpora lutea (8.72 ± 0.3 mm vs . 7.46 ± 0.9 mm) as well. Number of services per conception (2.6 vs . 1.4; p < .01) was higher in control group than that of phosphorus group. Pregnancy rate (80 vs . 50%) was significantly (p < .01) higher in phosphorus group when compared to control. White blood cells in treated ewes (10.8 ± 0.44; p < .05) and monocytes (2.93 ± 0.13; p < .01) were higher than that of control group (white blood cells; 9.53 ± 0.50 and monocytes; 2.24 ± 0.14). Metabolic parameters did not differ between phosphorus and control groups during different times of treatment. It could be concluded that phosphorous administration to anoestrous Farafra ewes in subtropics could improve reproductive performance and restore ovarian activity at the end of spring and early summer. 相似文献
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YANG Sheng-xi ZHANG Xiao-yan LUTong-de HA Xiao-qin WANG Hai-yan GENG Pai-li 《园艺学报》2008,24(5):1002-1006
AIM: To investigate the expression of natural killer cell stimulation receptor D(NKG2D) in peripheral blood and the expression of major histocompatibility complex class I chain-related protein A(MICA) on the gastric carcinoma tissue and the neighboring non-cancerous tissue in Tibetan patients with gastric cancer at Qinghai plateau. METHODS: Samples of 33 patients and 20 healthy persons were collected to detect NKG2D in peripheral blood by Flow cytometry analysis. The expressions of MICA in part of the correspondent tissues were examined by RT-PCR and immunohistochemistry.RESULTS: The expression of NKG2D in the plateau of Tibetan patients with gastric cancer group (13.47 ±5.26)% was significantly lower than that in healthy groups (32.62±10.08)%. The mRNA level of MICA in gastric cancer was significantly higher than that in neighboring non-cancerous tissue (P<0.05). The expressions of MICA proteins showed a significant difference between the neighboring non-cancerous tissue (21.21%, 7/33) and the gastric carcinoma tissue (78.79%, 26/33), between the high differentiation (60.00%, 3/5) and the moderate (72.73%, 8/11), low differentiation (88.24%, 15/17). The expressions of MICA were not correlated with tumor size, sex, age, lymph node metastasis of the patients (P>0.05). Spearman rank correlation displayed that the expressions of MICA mRNA were positively correlated with the MICA protein level (r=0.903, P<0.01).CONCLUSION: The immune escape in Tibetan patients with gastric cancer at Qinghai plateau probably relates to the down-regulation of NKG2D and the up-regulation of its ligand MICA. The activity of NK cells and the anti-cancer cellular immunity level are descending in the plateau of Tibetan patients with gastric cancer. The decrease in the receptor NKG2D is a reason for the activity of NK cell descending. 相似文献
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本研究对槟榔江水牛3-酮酸辅酶A转移酶1(3-oxoacid CoA-transferase 1,OXCT1)基因的完整CDS区进行了PCR扩增,并对其功能生物信息学和多组织差异表达进行了分析。以普通牛OXCT1基因序列(GenBank登录号:XM_006076397)为参考序列,采用Primer Premier 5.0软件设计引物序列,以提取的槟榔江水牛基因组DNA为模板,PCR扩增获得槟榔江水牛OXCT1基因mRNA序列,扩增产物经测序后利用ORF Finder软件进行开放阅读框识别,获得水牛OXCT1基因CDS区全序列,并对其进行生物信息学分析。结果显示,槟榔江水牛OXCT1基因CDS序列全长1 563 bp,编码520个氨基酸,蛋白分子式为C2509H4041N687O746S23,分子质量为56.50 ku,理论等电点(pI)为8.69,不稳定系数为27.49,平均疏水性(GRAVY)为-0.097,该蛋白属弱亲水性蛋白。OXCT1蛋白无信号肽和跨膜结构,属于线粒体膜蛋白;包含pcaJ_scoB_fam和AtoD 2个保守结构域,且具有5个功能活性位点。槟榔江水牛与普通牛、藏羚羊等5个物种的OXCT1氨基酸序列同源性≥ 97%。对槟榔江水牛13个组织进行表达谱分析表明,在泌乳期,OXCT1基因在乳腺中表达量最高,在肾脏、垂体、脂肪、大脑、皮肤和肌肉中不表达;在非泌乳期,OXCT1基因在除脂肪以外的其他12个组织中都有表达。OXCT1基因在槟榔江水牛泌乳期乳腺组织中表达量最高,推测其可能参与了水牛的乳脂合成调控事件。本研究结果可为深入了解乳脂合成代谢及其调控机制提供依据。 相似文献
5.
D. Liu Z. F. Pei M. S. Naeem D. F. Ming H. B. Liu F. Khan W. J. Zhou 《Journal of Agronomy and Crop Science》2011,197(4):284-295
The present study assesses the effects of 5‐aminolevulinic acid (ALA, 0, 0.1, 1 and 10 mg l?1) on the growth of oilseed rape (Brassica napus L. cv. ZS758) seedlings under water‐deficit stress induced by polyethylene glycol (PEG 6000, 0 and ?0.3 MPa). Water‐deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non‐enzymatic antioxidants (glutathione and ascorbic acid) levels. On the other hand, water‐deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, enzymatic antioxidants activities, reduced/oxidized glutathione ratio (GSH/GSSG) and reduced/oxidized ascorbic acid (ASA/DHA) ratio in seedlings. Application of ALA at lower dosages (0.1 and 1 mg l?1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings, whereas moderately higher dosage of ALA (10 mg l?1) hampered the growth. The study also indicated that 1 mg l?1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water‐deficit stress. Lower dosages of ALA (0.1 and 1 mg l?1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water‐deficit stress. The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l?1 ALA treatment under water‐deficit stress. It was also revealed that 1 mg l?1 ALA treatment alone induced the expression of APX, CAT and GR substantially and under water‐deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree. These results indicated that 0.1–1 mg l?1 ALA could enhance the water‐deficit stress tolerance of oilseed seedlings through improving the biomass accumulation, maintaining a relative high ratio of GSH/GSSG and ASA/DHA, enhancing the activities of the specific antioxidant enzymes and inducing the expression of the specific antioxidant enzyme genes. 相似文献
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基于北京大兴区耕地质量等级调查评价成果,按《土地质量地球化学评价规范》(DZ/T 0295—2016)补充采样调查测试,开展研究区特色农业产区土地质量地球化学评价.结果表明,研究区特色农业产区土壤养分综合等级主要以二等为主,面积为10266.18hm2,占特色农业产区总面积的53.67%;土壤环境质量总体优良,土地质量状况整体良好.特色农业产区土地质量地球化学评价结果可揭示特色农业产地土地质量等级以及重要土地问题,为区域特色农产品与生态农业开发等提供依据. 相似文献
8.
玉米多胞质雄性不育杂交种冀玉988的选育及利用 总被引:2,自引:0,他引:2
不育化制种主要在于保证杂交种子的质量及降低其生产成本。多胞质杂交种的利用主要拓宽胞质异质性,增强杂交种对环境的适应能力与防范小斑病菌新的专化小种的严重为害。本研究以优良玉米新品种冀玉988(6048×黄选921.6)为背景,以s组的s,M,R与21A及C组的Rb,Bb与Es雄性不育细胞质(CMS)为试材,将母本6048转育成同核异质体,不育性稳定。父本921.6对21A,s与R三种CMS具有强恢复性。本研究论证了多胞质杂交种在生产上利用的可行性。冀玉988已配制成具有恢复性能的多胞质雄性不育杂交种,可用于生产。 相似文献
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禾谷镰刀菌产生的细胞壁降解酶的特性研究 总被引:1,自引:1,他引:1
禾谷镰刀菌(Fusarium gramimearum<\i>)是引起玉米茎腐病、穗腐病的主要病原菌之一。该病原菌在活体外能产生PG、PMG、PGTE和PMTE四种细胞壁降解酶,这些酶都具有酶的典型性动力学特性。细胞壁降解酶是F.gramimearum<\I>致病的一个重要因子。 相似文献