农机液压技术的应用与发展

液压技术的应用已成为现代化农业机械的典型标志。为此,介绍了农机液压技术的应用现状;并根据科学技术的发展,简要分析了农机液压技术的发展趋势。     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

提高仔兔成活率的技术管理措施

从出生到断奶这段时间的兔称为仔兔,根据其生理特点,此阶段可分为睡眠期和开眼期,这是仔兔生长发育过程中非常重要而又很难饲养的时期.在养兔生产中,仔兔成活率的高低对经济效益有很大的影响,直接关系到养兔的成败.因此,加强仔兔饲养管理,采取有效的保护措施,提高仔兔成活率是养兔成功的关键.     

贵州省黔西县旱耕地质量状况评价

为了给测土配方施肥提供基础数据,对黔西县项目区的旱耕地的土壤质量进行了初步评价。黔西县旱耕地质地主要以壤土为主,土壤pH 5.5~8.5,比较适宜作物生长。旱耕地营养状况:土壤肥力水平中等,总体上普遍缺氮,中度缺磷,钾较丰富,钙、镁、硫、铁含量极丰富;锰含量比较丰富,但分布不均,变异较大;铜10%调查点缺乏,锌20%左右调查点缺乏,硼80%以上调查点缺乏。     

基于NGO-CNN-SVM的高标准农田灌溉工程施工成本预测

为提高高标准农田项目施工成本的预测精度,控制施工成本在合理范围,减少投资风险,该研究从单体灌溉工程施工成本预测角度出发,通过随机森林（random forest,RF）筛选出高标准农田灌溉工程施工成本的关键影响因素,结合卷积神经网络（convolutional neural networks,CNN）和支持向量机（support vector machine,SVM）两种模型的优点,通过北方苍鹰优化算法（northern goshawk optimization,NGO）对模型里的惩罚因子和核参数进行寻优,构建基于NGO-CNN-SVM的施工成本预测模型。通过辽宁省2018—2023年高标准农田工程中灌溉工程的施工成本数据,选取样本决定系数R²、平均绝对误差MAE、平均绝对百分比误差MAPE和均方根误差RMSE作为精度指标进行分析,结果表明：基于NGO-CNN-SVM的施工成本预测模型在渠道工程中MAE低于0.615万元,RMSE低于0.512万元,R²达到0.968以上,相对误差小于4.210%;在进水闸工程中MAE低于0.610万元,RMSE低于0.536万元,R²达到0.966以上,相对误差小于4.410%;在桥涵工程中MAE低于0.494万元,RMSE低于0.477万元,R²达到0.970以上,相对误差小于3.548%,并相比较于反向传播神经网络,CNN和CNN-SVM模型,NGO-CNN-SVM模型的预测结果均最优。通过特征选择、模型融合、算法优化以及不同模型对比表明NGO-CNN-SVM模型具有更高的预测准确率和泛化性,可为高标准农田灌溉工程施工成本预测提供理论依据。     

不同形态钾肥对马铃薯产量及品质的影响

采用随机区组设计,研究了在施入等量钾肥的前提下,硫酸钾及氯化钾配施对马铃薯植株生育性状、氨基酸、钾含量等品质和产量及商品薯率的影响.结果表明,等量不同形态钾肥对植株株高和主茎数有一定的影响,对其他生育性状基本无影响;对块茎中蛋氨酸含量无显著影响,对其他类型氨基酸、总氨基酸、还原糖和钾含量有一定影响;对马铃薯产量、经济效...     

秸秆覆盖量和覆盖时间对党参生长及光合特性的影响

试验以露地不覆盖(CK1)和地膜露头覆盖(CK2)为双对照,设置了移栽覆盖(A1)和出苗覆盖(A2)2个覆盖时间,4500 kg·hm-2(B1)、7500 kg·hm-2(B2)、10500 kg·hm-2(B3)、13500 kg·hm-2(B4)4个覆盖量,采用二因素随机区组设计,研究了秸秆覆盖量和覆盖时间对党参...     

茶树LOX基因家族的鉴定及其在白茶萎凋过程的表达分析

脂肪族类化合物是植物芳香物质的重要组成部分,对白茶香气的形成具有重要作用.利用生物信息学方法,在染色体级别的茶树基因组数据库中,对LOX基因家族进行鉴定,从中获得12个茶树LOX基因家族成员,命名为CsLOX1～CsLOX12.12个茶树LOX基因序列,主要定位于细胞质或叶绿体中,其编码蛋白具有相同的特征结构域及保守基...     

龙湖森林公园景观资源与景区规划构想

详细介绍构成龙湖省级森林公园景观资源的五大类型:生物、水域、天象、地文和人文景观资源.依据公园的性质、规划原则进行总体布局,并分别对规划的观光游览、休闲度假、激情游乐、悠游探秘四大景区的特点和园林绿化规划内容进行阐述.提出在保护自然环境的前提下,实行利用与保护兼顾,突出特色,将其规划建设成为一个兼有避暑、度假、休闲、娱乐、科普教育于一体的生态型多功能省级森林公园.