福建省森林公园建设现状、存在问题及解决对策

阐叙福建省森林公园建设发展现状、并着重对森林公园建设发展、资金投入、景观开发、森林景观、经营管理、管理体制等存问题提出相应的解决对策。     

东北虎雌性生殖系统解剖

对 2例 1 999年 1 0月意外死亡的东北虎雌性生殖系统进行了解剖。雌性东北虎的卵巢被覆卵巢囊 ,随着年龄增长卵巢表面凸凹不平 ,呈脑的沟回状。输卵管位于卵巢游离缘外侧 ,呈半圆形围绕着卵巢 ,输卵管壶腹部弯曲、峡部直 ;子宫与输卵管分界明显 ;子宫角长度约相当于子宫体长度的 2倍 ;阴道呈背腹压扁的管状     

关于水稻机插秧技术在泰和县试验示范的探讨

介绍了江西省泰和县进行水稻机插秧技术与手工插秧对比试验。对不同农艺的经济效益和不同育秧方式的适应性进行了分析，并指出了目前技术措施存在的不足之处。     

兽药使用环节存在的问题及对策

经过对兽药生产厂家和经营企业几年的整治,尤其是新<兽药管理条例>和<兽药标签和说明书管理办法>出台后,又经过GMP认证,兽药生产经营市场秩序和经营行为得到了一定的规范.但兽药使用环节中却存在诸多的问题,如:违法使用人用药品;违法使用禁用兽药;不执行休药期规定;未建立台帐等等.这些问题的存在,直接导致了动物产品的安全性问题.对此,笔者分析了兽药使用环节存在问题的原因,并对解决这些问题提出了个人观点.     

Caspase非依赖性细胞死亡的研究进展

细胞凋亡是一种生理性细胞死亡机制，它是由多基因调控的一种细胞的主动的自杀性过程。一般认为它是由蛋白酶caspases通过两种途径激活的。但是，近年来研究发现除caspases引起的细胞凋亡外，还有caspases非依赖性的细胞死亡形式存在，包括细胞的自我吞噬作用、细胞蛋白酶体的降解和线粒体途径。这些途径无论是在试验的条件下还是在生理或病理的情况下都可以观察到。目前认为凋亡诱导因子(AIF)在caspases非依赖性的细胞死亡中起关键作用。     

Therapeutic effects of recombinant human growth hormone on rat sepsis

AIM:To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms.METHODS:Mean arterial pressure (MAP), levels of plasma TNFα, IL-1β and endotoxin, leukocyte count and survival rate within 1 week were determined after E. coli injection among control group, sepsis group and sepsis+rhGH group.RESULTS:(1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNFα levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1β level among the three groups.CONCLUSION:rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function;maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNFα.     

High efficient expression of human endostatin in E.coli and its antiangiogensis activity

AIM: To examine the expression of human endostatin in E.coli, produce its fusion protein antibody and observe its biological activity. METHODS: Endostatin gene was amplified by polymerase chain reaction,recombined with plasmid vector pGEX-2T and induced expression with IPTG.The protein activity was tested by endothelial cell proliferation inhibitory assay.Inclusion body crudely purified was used to generate polyclonal antibody to detect its expression at mouse's liver and kidney etc. RESULTS: The protein expressed was 20kD after digestion by thrombin,it appeared the anti-angiogenesis activity and Western blotting indicated the expression of endostatin in liver and kidney of mouse. CONCLUSION: The successful expression of human endostatin and the preparation of polycolonal antibody indicated its potential application in anti-angiogenesis therapy and diagnosis tumors.     

不同培养条件对鲍鱼菇菌丝生长的影响

通过不同温度、pH值及培养基含水量处理 ,对鲍鱼菇菌丝生长状况进行了研究。结果表明鲍鱼菇菌丝生长适宜的温度范围为 2 0～ 31℃ ,最适温度为 2 5～2 8℃ ;pH值适宜的范围为 4 0～ 7 0 ,最适pH值为 5 5～6 0 ;培养基含水量适宜的范围为 6 0 %～ 80 % ,最适含水量为 80 %。     

Cortical gene expression pattern in rat focal cerebral ischemia

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.     

Effect of propolis on the expression of CD54 and activation of NF-κB p65 of lung tissue in acute lung injury rats

AIM: To study the effect of propolis on the expression of CD54 and activation of NF-κB p65 in lung tissue of acute lung injury (ALI) rats. METHODS: 40 male Wistar rats were divided into 5 groups: normal control, model control, dectancyl group, water soluble derivative of propolis (WSP) group and ethanol extracted propolis (EEP) group. ALI animal model was performed by oleic acid and LPS twice attack. The pathologic slice was observed with light microscope and the NF-κB p65 activity and CD54 expression were tested by immunohistochemistry (SABC and SP). RESULTS: Both EEP and WSP antagonized the lung edema, decreased the inflammation and inhibited the expression of CD54 and activation of NF-κB p65. CONCLUSION: The increase in the expression of CD54 and the activation of NF-κB p65 in the lung tissues of ALI were involved in the formation of ALI. Propolis ameliorated the lung damage, which maybe related to the inhibition of CD54 expression and NF-κB p65 activation.