燕麦饲草多组分营养差异分析

为挖掘利用高营养燕麦饲草种质资源,将源自以色列不同地区的23份野生燕麦生态型和3份燕麦栽培品种种植于四川甘孜、贵州贵阳、四川成都3个环境,分别在抽穗期、灌浆期、成熟期进行刈割处理,并测定其酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)、酸性洗涤木质素(ADL)、纤维素(F)、半纤维素(HF)、木质素(L)、洗涤纤维中性...     

棉花优良品种中棉所27直接胚胎发生与植株再生

选用我国难培养基因型优良棉花品种中棉所27进行组织培养,研究了该品种体细胞胚胎发生与植株再生能力。该品种体细胞胚胎发生有2种情况:一是由外植体直接诱导获得胚性愈伤组织并形成不同发育时期的胚状体;二是先诱导获得非胚性愈伤组织,再经多次继代培养,获得胚胎发生。不同激素及其组合和浓度配比是影响中棉所27直接诱导获得胚性愈伤组织及体细胞胚胎发生的直接因素。另外,饥饿培养及继代周期对胚胎发生也有重要影响。0.1mg/LKT和0.1mg/LIAA组合、0.1mg/LKT和0.1mg/LZT组合、0.1mg/LZT单独使用,在饥饿条件下培养,均能直接高效诱导获得大量胚性愈伤组织,并有短期内形成不同发育时期的胚状体。胚状体在不含任何激素的MS B5培养基上培养有利于植株再生,并降低畸形苗形成率。另外,还发现不同培养基对中棉所27胚性愈伤组织增殖、体细胞胚形成和萌发的影响不同。     

云南省昆明地区药用种子植物资源与多样性研究

通过查阅各种资料文献、野外标本采集和实地考察等方法,对云南省昆明地区药用种子植物资源进行系统、详细调查以及多样性分析,结果表明:昆明地区药用种子植物共计141科415属599种(包括变种),被子植物129科384属343种,裸子植物12科31属256种,其中优势科为蔷薇科、菊科和百合科,优势属为菊属、蒲公英属、水仙属和君子兰属;药用部位方面根、根茎类所占比例较高,其次为全草类.基于上述研究分析结果提出对昆明地区药用种子植物资源开发利用和保护建议.     

负荷建模参数辨识中综合改进遗传算法的应用

参数辨识是负荷建模的关键之一,针对遗传算法本身存在的缺陷,提出一种综合改进的遗传算法.该算法通过初始种群选择、最优个体保留、自适应交叉和变异率等方面进行综合设计,有效地避免了早熟和近亲繁殖,提高了收敛速度.建模实践表明,所提的综合改进遗传算法可加速收敛,缩短辨识时间,同时提高了拟合精度,克服了参数的分散性,是一种适合于负荷建模参数辨识的优化算法.     

Effects of Yangxue-Jiedu decoction on imiquimod-induced psoriasis-like lesions in STAT3 transgenic mice

AIM: To observe the effects of Yangxue-Jiedu (YXJD) decoction on imiquimod-induced psoriasis-like lesions in STAT3 transgenic mice.METHODS: STAT3 transgenic mice (n=24) were randomly divided into 4 groups: control group (using purified water for oral administration), model group (topical 5% imiquimod 42 mg and using purified water for oral administration), YXJD groups (topical 5% imiquimod 42 mg and using YXJD decoction for oral administration), and methotrexate (MTX) group (1 mg/kg MTX solution for oral administration, with the same topical imiquimod as model group). On day 7, the skin lesions were collected for examination. The lesions were evaluated according to the psoriasis area and severity index (PASI). The skin barrier function was evaluated by assessing oil and water components in the skin. The inflammation of psoriasis-like lesions was assessed by histological method. The expression of proliferating cell nuclear antigen (PCNA) and CD3 was assessed by immunohistochemical staining. The mRNA expression of IL-17A, IL-17C, IL-22 and RORγt was detected by real-time PCR. The levels of JAK/STAT3 pathway-related proteins in isolated T cells were determined by Western blot.RESULTS: Administration of YXJD decoction inhibited imiquimod-induced keratinocyte proliferation and infiltration of CD3⁺ T cells in psoriatic lesions, and ameliorated the epidermal barrier by up-regulation of the oil and water components in psoriatic lesions. Meanwhile, administration of YXJD decoction improved the systemic immune responses by reducing the weight of the spleen. The inflammatory cytokines IL-17A, IL-17C, IL-22 and RoRγt, and the levels of JAK/STAT3 pathway-related proteins STAT3, p-STAT3, JAK3 and p-JAK3 were decreased by administration of YXJD decoction.CONCLUSION: YXJD decoction likely alleviates imiquimod-induced psoriasis-like lesions in the STAT3 transgenic mice by inhibiting the phosphorylation of STAT3, and reducing the expression of IL-17A, IL-17C, IL-22 and RORγt.     

Effect of autophagy on necroptosis of renal tubular epithelial cells in subtotal nephrectomy rats

AIM: To explore whether autophagy is involved in the excessive death of renal tubular epithelial cells in subtotal nephrectomy(SNx) rats and the relationship between autophagy and necroptosis in the kidney of SNx rats. METHODS: Male Sprague-Dawley rats were randomly assigned to control group(n=6) and SNx group(n=42). The rats in SNx group were subjected to SNx. Sham surgery was performed in the rats in control group. The rats in SNx group were divided into subgroups at 0, 4, 8 and 12 weeks(n=6) and the other rats in SNx group were divided into SNx+vehicle group, SNx+necrostatin-1(Nec-1) group and SNx+3-methyladenine(3-MA) group. The expression of RIP1, RIP3, LC3 and beclin-1 at mRNA and protein levels was measured at 0, 4, 8 and 12 weeks by qPCR and immunohistochemistry. The effects of Nec-1 or 3-MA on the protein expression of LC3-I, LC3-II and beclin-1, and production of reactive oxygen species(ROS) in the rat kidney were determined by Western blot and DCFH-DA staining. The death of renal tubular epithelial cells in the SNx rats was observed by TUNEL staining and electron microscopy. Finally, the effects of Nec-1 and 3-MA on blood urea nitrogen(BUN), serum creatinine(SCr) and the pathological changes of the renal tissues were analyzed. RESULTS: The highest mRNA and protein levels of RIP1, RIP3, LC3 and beclin-1 appeared at the 8th week after SNx(P<0.01). Compared with the rats in SNx+vehicle group, the protein over-expression of LC3-II/I and beclin-1, renal tubular epithelial cells with typical morphological features of necroptotic cell death and TUNEL-positive renal tubular cells were decreased in the SNx rats treated with Nec-1 and 3-MA(P<0.01), but 3-MA did not reduce the increased concentration of ROS. In addition, treatment with Nec-1 and 3-MA obviously reduced BUN, SCr(P<0.05), glomerulosclerosis index and tubulointerstitial injury score(P<0.01). CONCLUSION: Autophagy participates in the excessive death of renal tubular epithelial cells in SNx rats. Inhibition of autograph prevents necroptotic cell death of renal tubular cells, and alleviates chronic renal injury in SNx rats.     

猪圆环病毒II 型 rep蛋白的亚细胞定位生物信息学析

从GenBank 发表的PCV-2全基因组序列,随机选择其中的32 个全基因序列,做序列比对和同源性比较,绘制系统进化树,在注释中找到ORF1编码的rep蛋白氨基酸序列, 用在线生物信息学工具对其进行亚细胞定位分析.结果基本符合"来自同一地区的基因序列同源性较高"的规律,表明了PCV的高度保守性, 亚细胞定位分析发现其rep蛋白定位于核区的指数为0.76,这与病毒在宿主细胞核内复制分不开.结果同时表明其定位于过氧化物酶体的指数显著不同于其他(过氧化物酶体平均0.36,可变,溶酶体、线粒体均为0.1不变)这可能与pcv的组织嗜性以及复制靶细胞(多为单核/巨噬细胞)的原因相关.     

民族混居地区社区经济发展的现状与对策——以楚雄市苍岭镇李家村汉、回、彝族混居为例

如何实现多民族混居地区经济社会和谐发展,走出一条具有自己民族特色和地域特色的科学发展之路,是当前多民族混居地区面临的重大理论和现实问题。以李家村汉、回、彝族混居的居住区为例,对李家村多民族混居地经济社会发展存在的问题进行探讨,并给出了几条可行性建议。     

不同棱型大麦品种(系)籽粒灌浆特性和产量性状分析

以河南省的主栽品种和正在培育品系为材料,通过大田试验,拟合大麦灌浆过程生长曲线,分析其参数变化情况。结果显示,大麦籽粒灌浆进程均呈“慢—快—慢”的“S”型曲线,拟合的Logistic方程决定系数均大于0.9906,并达到极显著水平;籽粒灌浆过程中缓增期持续时间较长,快增期持续时间次之,渐增期时间较短,快增期平均灌浆速率最高,其最终干物质积累量也明显高于渐增期和缓增期。二棱型品种(系)的3个时期平均灌浆速率(‘驻3’的V1除外)和最终干物质积累量均高于多棱型品种(系);各品种(系)实际千粒重均低于理论千粒重。在穗数和千粒重协同下,多棱型品种(系)穗粒数高的最终产量高,而二棱型品种(系)穗部籽粒数低于多棱型品种(系),在一定的穗数下,千粒重高的产量相应较高。     

珠美海棠MzWRKY基因家族盐胁迫应答模式研究

对苹果属植物现有的58个WRKY 的EST序列进行分析,得到了37条uniESTs。珠美海棠（Malus zumi Mats）是耐盐的优良苹果砧木,能在土壤全盐含量为0.6%的盐碱地上存活。根据已知的37条uniESTs序列,通过半定量RT-PCR研究了珠美海棠中MzWRKY基因家族的盐应答模式。28个MzWRKY基因能检测到RT-PCR的产物,其中21个基因受盐诱导,1个受盐抑制。根据MzWRKY基因盐应答模式可将这些基因分为两组,基因表达模式的差异说明MzWRKY在珠美海棠的盐应答中角色的多样性。