Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.     

治疗图书馆顽症:流程重组

本文将流程重组理论引入图书馆管理，分析了图书馆流程重组的必要性，同时具体阐述了图书馆流程重组的三种方式。     

乳铁蛋白及其生理机能的研究进展

乳铁蛋白作为一种天然的具有免疫功能的糖蛋白,是转铁蛋白家族的一员。它存在于哺乳动物的各种外分泌物中,乳中的含量较高,因其广泛的生理功能,近年来越来越多地受到人们的关注。概括介绍乳铁蛋白的结构及其重要的生理功能以及乳铁蛋白的制备方法,并展望乳铁蛋白作为一种添加剂在畜牧业上的应用前景。     

两种兼用型牛BoLA-DRA*exon2多态性及其与乳房炎的关联分析

利用PCR-SSCP技术,分析了牛主要组织相容性复合物DRA基因外显子2的多态性在236头中国西门塔尔牛、三河牛中的基因型分布及其多态性与乳房炎的关系。结果表明:经χ2独立性检验,发现该多态片段6种基因型在感染乳房炎牛和健康牛之间分布不一致(p<0.01)。在感染乳房炎牛中CC基因型频率最高,在健康牛中BC基因型频率最高。据此推测,CC基因型可能与这两种牛的乳房炎易感性有关,而BC基因型可能与这两种牛的乳房炎抗性有关。     

"雅士勇"口服液对猪的射精量和精子畸形率的影响

以大白猪和长白猪为研究材料,对照组不饲喂"雅士勇"口服液,两试验组分别饲喂25 mL和30 mL"雅士勇"口服液,研究"雅士勇"口服液对猪的射精量和精子畸形率的影响。结果表明,"雅士勇"口服液对射精量无明显影响(P>0.05),但对种公猪的精子畸形率有显著影响,对照组的精子畸形率均极显著地高于两个试验组(P<0.01),而两试验组之间精子畸形率差异不显著(P>0.05)。     

亚砷酸氧化菌Sinorhizobium sp. GW3的鉴定与亚砷酸氧化酶基因的分离

亚砷酸氧化细菌能够将毒性大的As(Ⅲ)氧化成毒性小的As(Ⅴ),在生物修复砷污染方面具有应用价值。对一株新型亚砷酸氧化菌Sinorhizobium sp.GW3进行了较全面的鉴定,并分离及分析了亚砷酸氧化酶基因aoxAB。形态学、生理生化鉴定和16S rRNA基因等分析结果表明该菌为Sinorhizobium属。该菌对As(III)抗性的MIC(Minimum inhibitory concentration)为9 mmol/L。首次在Sinorhizobium属中分离了包括编码小亚基aoxA和大亚基aoxB在内的亚砷酸氧化酶基因,其编码的大小亚基与已发现的Agrobacterium tumefaciens(ABB51929)的大小亚基在氨基酸水平上分别有86%、80%的同源性。     

基于渔捞日志的长江靖江段刀鲚渔获量的时空特征分析

江苏靖江段位于近长江口段,是长江刀鲚渔汛最集中的水域。为了弄清靖江段刀鲚的渔汛特征,本文于2008-2009年和2012-2013年对16艘持刀鲚捕捞许可证的渔船作了渔获量监测,分析了渔获量的时空变化及其环境影响。结果表明,靖江段单船日渔获数量NB和重量WB分别为(21±38)ind./d和(2.0±4.1)kg/d,单船全汛总渔获尾数Nt和重量Wt为(890±929)ind.和(92.3±91.1)kg。NB和Nt具有一致性的年变化趋势,以2013年的最大,其他年份比较接近。但WB和Wt的年变化趋势与渔获数量的年变化趋势有所不同,表现为2008-2012年间持续下滑,2013年显著增长。NB和WB在2-3月间均极低,但4月增至(23.0±31.3)ind./d和(2.4±3.5)kg/d,5月达(78.0±81.0)ind./d和(7.7±9.1)kg/d。ANOVA分析显示,西水域的年NB和WB分别为东水域的2.5和2.7倍。研究亦显示,靖江段刀鲚的WB与同日水温和最高潮位均呈极显著的正相关(P﹤0.05)。长江口外的水温提升,可能是刀鲚开始生殖洄游的重要环境诱导因子。而高潮期在靖江段出现最高渔汛,可能是因为所采用的固定刺网过滤了更多的江水所致。     

植物根系分泌物对污染胁迫响应的研究进展

根系分泌作用是植物实现对外交流的重要形式,研究根系分泌物对环境胁迫的响应是明确植物环境适应性机制的重要途径。本文综述了植物根系分泌物对重金属污染和有机物污染的响应特点,分析了根系分泌物应对重金属和有机物污染胁迫的机制,对污染胁迫下根系分泌作用的研究和应用进行了展望,并讨论了需要进一步研究的问题。     

双孢蘑菇贮藏条件对其生理生化变化影响的研究进展

贮藏条件是影响双孢蘑菇贮藏效果的重要因素,介绍了气体成分、温度和湿度等因素对双孢蘑菇生理生化变化的影响。     

mTOR kinase inhibitor CC-223 suppresses human breast cancer cell viability

AIM: To investigate the inhibitory effect of CC-223, an inhibitor of mammalian target of rapamycin (mTOR) kinase, on the viability of human breast cancer cells and its mechanism. METHODS: The inhibitory effect of CC-223 on the viability of MCF-7 cells and MDA-MB-231 cells was measured by CCK-8 assay. The cell cycle distribution of breast cancer cells was examined by flow cytometry. The expression of cell cycle-related proteins and oncoproteins c-Myc and survivin was analyzed by Western blot. RESULTS: CC-223 significantly inhibited the viability of both MCF-7 and MDA-MB-231 cells (P<0.05). CC-223 induced cell cycle arrest in both G₁ phase and G₂/M phase in the MCF-7 cells (P<0.05). However, low concentration of CC-223 treatment resulted in the accumulation of MDA-MB-231 cell cycle in the G₂/M phase, and the cell number in G₁ phase was unaffected. Treatment with CC-223 for 24 h clearly inhibited the protein levels of cyclin B1, cyclin D1 and phosphorylated cell division cycle protein 2 in the breast cancer cells (P<0.05). CC-223 suppressed the expression of c-Myc and survivin in both MCF-7 cells and MDA-MB-231 cells (P<0.05). CONCLUSION: CC-223 inhibits cell viability by blocking cell cycle progression and down-regulating expression of c-Myc and survivin in both MCF-7 and MDA-MB-231 cells.