治疗图书馆顽症:流程重组

本文将流程重组理论引入图书馆管理，分析了图书馆流程重组的必要性，同时具体阐述了图书馆流程重组的三种方式。     

入世对我国就业的影响

中国目前的就业形势是严峻的,入世对我国就业会产生重大影响。在中短期内,主要表现为消极影响,会增加就业的压力;而从长远看,会使我国体制改革、市场开放程度等因素发生变化,从而增加就业机会,缓解就业压力。     

基于工作流的ERP系统重构的研究

分析了企业现有ERP系统运营存在的缺陷，提出了适合本企业的基于工作流的ERP系统重构的开发方案。     

The role of adrenomedullin in diabetic nephropathy

AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-β₁ in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM、 TGF-β₁、 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-β₁ and reducing excessive accumulat ion of laminin and type IV collagen.     

长期培肥我国典型黑土玉米氮肥效应的演变趋势

【目的】依托中国东北典型农田设置的公主岭黑土长期培肥试验,研究长期培肥条件下黑土地区玉米产量和氮肥效应的时间演变趋势,阐明氮肥利用率和土壤养分之间的相关性,为指导我国建立长期培肥模式和提高氮肥利用率提供依据。【方法】本文选择不同有机肥用量(M0、M30和M60)下不施肥处理(CK)、施氮肥(N)、施磷钾肥(PK)和平衡施肥(NPK),共计12个处理,研究长期培肥对玉米的氮肥农学效率、氮肥当季利用率和氮肥累积利用率的影响。【结果】有机肥料和化肥(NPK)对玉米均具有显著增产效果,有机肥化肥配施可降低玉米产量的变异系数(CV)和提高可持续性指数(SYI)从而保证玉米的持续稳产高产。平衡施肥条件下的氮肥农学效率、当季利用率和累积利用率随着施肥年限的延长呈上升趋势并且达到显著或极显著水平;常量有机肥区和高量有机肥区的土壤肥力得到显著提升,氮肥效应随时间演变趋势分别为变化不显著和显著降低趋势。氮肥利用率与土壤有机碳、土壤全氮、土壤全磷和土壤速效钾呈显著负相关。【结论】在相对较高的肥力条件下,黑土地区玉米氮肥效应呈显著下降或变化不显著的趋势将为高肥力条件下有机肥部分替代化肥提供科学依据。     

参麦与黄芪注射液联用治疗卡氏肺孢子虫肺炎

为研究参麦与黄芪注射液联用对大鼠卡氏肺孢子虫肺炎(PCP)的治疗效果,以地塞米松连续肌肉注射Wistar大鼠6周,建立PCP大鼠模型,用参麦与黄芪注射液皮下注射治疗患病大鼠,同时设复方磺胺甲恶唑(SMZ-TMP)治疗对照组和PCP模型阳性对照组。通过各组大鼠基本情况变化、肺组织印片检查包囊、肺组织病理切片染色观察组织变化,探讨其治疗效果。结果发现,参麦与黄芪注射液联用治疗后,大鼠饮食、饮水增加,活跃程度恢复,体重不同程度增加,肺印片Pc包囊数量比治疗前明显减少,肺组织炎症反应减轻,高剂量组治疗效果接近复方磺胺甲恶唑组。研究结果表明,参麦与黄芪注射液对肺孢子虫有明显的治疗作用。     

Construction and Identification of Yeast Surface Display Libraries of Ovis aries DRA Gene Exon 2

The specific primers were designed according to Ovis aries DRA gene sequence deposited in GenBank and the multiple cloning site of the plasmid pYD1,which was a vector used for protein surface display on Saccharomyces cerevisiae.The gene encoding DRA was amplified by PCR using the genomic RNA of Ovis aries.The 762 bp fragment was cloned and released in GenBank and registration number was KR422362.The PCR product was inserted into the yeast surface display plasmid vector pYD1 by double enzyme digestion.It was indicated that DRA gene was successfully integrated into the genome.Dot mutation was made at both ends of exon 2 in DRA gene for making restriction enzyme cutting site and design the exon 2 specific primers according to mutated Ovis aries DRA gene sequence.Sequenced exon 2 amplification products based on DNA pooling of sheep large sample template was analyzed the polymorphic loci.The polymorphic exon 2 246 bp fragment was obtained by double enzyme digestion and connected to surface display restructuring mutation carriers pYD1-DRA by the same double enzyme digestion,and then we successfully constructed yeast surface display libraries.We transformed it into Saccharomyces cerevisiae EBY100 cell.Yeast monoclone was identified by PCR amplification and sequencing,and we confirmed that DRA gene had been integrated into Saccharomyces cerevisiae genome.After galactose induced,it was detected that DRA gene library had been successfully demonstrated on the yeast cell surface under the fluorescence microscope by immunofluorescence method.     

Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

叶面喷肥对‘早金酥’梨果实有机酸含量的影响

以‘早金酥’梨为试材,在生长季节叶面喷施氮、磷、钾、钙、镁和稀土肥,用高效液相色谱法测定成熟果实主要有机酸含量,研究不同矿质营养对梨果实总有机酸含量的影响。结果表明,叶面喷施氮肥可以使果实奎尼酸、柠檬酸和总有机酸含量显著增加,而乳酸含量显著降低;叶面喷施磷肥可以使果实苹果酸、草酸和总有机酸含量显著降低;叶面喷施钾肥可以使果实苹果酸、奎尼酸和总有机酸含量显著增加,而莽草酸和乳酸含量显著降低;叶面喷施钙肥可以使果实苹果酸、柠檬酸和总有机酸含量显著降低;叶面喷施镁肥可以使果实奎尼酸、柠檬酸和总有机酸含量显著增加;叶面喷施稀土肥可以使果实奎尼酸、柠檬酸、莽草酸、乳酸和总有机酸含量显著降低。     

植物开花光周期反应的分子调控机制

植物开花时间受到日照长短季节性变化的调节,拟南芥和水稻中与光周期反应相关基因的分离,使人们得以认识植物开花光周期反应的分子调控机制。植物感知日照长短的变化主要由CONSTANS(CO)基因的表达所控制。CO能够将光信号与生物钟信号整合,调节开花基因FLOWERING LOCUS T(FT)的表达,并最终控制植物的开花时间。本文对这一研究的最新进展进行了综述。