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1.
为研究草莓中SCF复合体的功能,以栽培草莓品种‘74’为试材,采用同源克隆的方法分离出2个SKP1基因。这2个基因核苷酸序列全长均为519bp,核苷酸序列相似性为98.46%,氨基酸序列相似性为98.84%,并具有特殊的‘GVDED’尾巴结构,分别命名为FaSKP1-1a和FaSKP1-1b(基因登录号分别为KU975057和KU975058)。RT-PCR和dCAPS分析发现FaSKP1-1a和FaSKP1-1b在草莓根、茎、叶、花托、花粉、花柱、果实中均高表达,在花瓣中表达量低。上述结果表明FaSKP1-1可能在草莓SCF复合体的形成过程中发挥重要作用。 相似文献
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种质资源是遗传研究与作物改良的基础。饲草产量与品质是决定饲草型小黑麦品种利用价值的重要指标。本研究以113份国内外小黑麦种质为材料,通过2年田间种植,对其饲草产量与品质性状进行了分析与评价。结果表明参试小黑麦种质的饲草产量及其品质性状存在极显著差异,其单株鲜草产量分别为36.000~111.560 g与36.310~159.780 g,单株干草产量分别为12.000~27.000 g与9.150~30.150 g,鲜干比分别为2.380~4.370与2.610~6.210,饲草粗蛋白含量分别为6.894%~13.259%与6.680%~14.304%,饲草中性洗涤纤维含量分别为48.480%~74.850%与53.850%~67.980%,酸性洗涤纤维含量分别为26.600%~42.780%与29.000%~39.280%,饲草的相对饲用价值分别为69.650~128.150与79.840~113.170。饲草产量与品质性状的多样性指数范围为1.974~2.075。主成分分析表明,饲草纤维品质因子、饲草产量因子与综合品质因子为前3个主成分,其累计贡献率为82.198%。依据单株干草产量... 相似文献
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误差修正是实时洪水预报研究和应用中的重要内容,从修正模型降雨输入角度出发,提出了一种基于微分响应的降雨误差修正方法,并通过实例论证了该方法修正降雨量的适用性。该方法通过计算降雨所对应模型的微分响应来修正降雨,从而修正出口断面流量过程。将该修正方法结合新安江模型,对闽江建阳流域的19场历史洪水进行了有效性检验,结果表明:此方法对洪量、洪峰的修正效果明显,且能够显著提高洪水预报的合格率,修正后合格率由63.2%提高到94.7%。 相似文献
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典型煤矿工业园区土壤重金属污染评价 总被引:3,自引:1,他引:3
随着工业化进程的加快,一些农业用地转为工业用地,土地用途的转变可能会影响周围土壤的质量。该研究以河南省东部某煤矿工业园区为对象,分成不同小区采集土壤样品,分析工业园区内表层土壤的Hg、Cd、Zn、As、Pb、Cr六种重金属含量及污染评价,并同时与周边土壤重金属含量进行比较,分析可能造成土壤环境污染的因子。结果表明,根据土壤环境质量标准,工业园区域内土壤污染指数小于0.7,土壤清洁;但与周边土壤比较存在污染的风险,潜在重金属威胁主要表现为Hg,其他重金属污染风险趋势为Cd>Zn>As>Pb>Cr;在空间分布上,除Cr外,随着与工业园区距离的加大,其他五种重金属其含量有降低的趋势;同时根据结果提出合理土壤污染防控建议。 相似文献
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对3个玉米自交系遗传差异不同的株系自交3代后株系间产量及相互间杂交优势进行分析。结果表明,同一自交系在遗传上存在微小差异的株系,产量水平及其产量构成因素仍然存在一定差异。自交系初始遗传差异越大,后代株系间的产量差异越大;初始单株遗传差异较小的两个玉米自交系昌7-2和郑58具有微小遗传差异株系间杂交组合的超中亲优势分别达到10.55%、4.77%,超高亲优势分别达到3.69%、3.94%。对杂交优势贡献主要来自行粒数,其次是千粒重。通过关联分析找出行粒数相关基因的SNP标记14个(-Log P4),千粒重相关基因的SNP标记3个(-Log P3)。在开放授粉的条件下,株系间微小遗传差异有利于提高亲本和杂交制种产量。 相似文献
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CAI Chen-chen YE Li-xia ZHU Jiang-hu BAI Jun-jie ZENG Shan-shan CHEN Shang-qin LIN Zhen-lang 《园艺学报》2019,35(2):311-319
AIM:To investigate whether ellagic acid (EA) attenuates hypoxic-ischemic encephalopathy (HIE) by down-regulating autophagy. METHODS:In vivo, Sprague-Dawley rats (n=17) were randomly divided into 3 groups:5 rats for sham group, 6 rats for HIE group and 6 rats for HIE+EA pretreatment group. The rats in HIE+EA pretreatment group were treated with EA (10 mg/kg, 10 mL/kg, suspended in corn oil, ig). After 24 h of operation, the rats from each group were sacrificed and their brains were collected. TTC staining and HE staining were used to define the infarct areas and brain structure. The autophagy-related proteins beclin-1, P62, LC3-Ⅱ/-I and Atg5 in the cortex in each group were compared by Western blot. In vitro, PC12 cells were divided into 3 groups:control group, CoCl2 group and CoCl2+EA pretreatment group. CoCl2 at 800 μmol/L was added to the PC12 cells to induce an anoxic environment. The PC12 cells were pretreated with EA at 8 μmol/L and the cell viability was measured by CCK-8 assay. The production of reactive oxidative species (ROS) in the cells was detected by flow cytometry with DCFH-DA staining. MDC staining and TMRE staining were applied to reflect the extent of autophagy and the state of apoptosis, respectively. The autophagy-related proteins in PC12 cells were also investigated. RESULTS:In HIE group, 7-day-old rats were given the operations and the their large infarct areas in the hemisphere were observed by TTC staining. HE staining displayed the injured hemispheres which contained few neurons, and exhibited edema status and serious structural damage. EA pretreatment decreased the infarct area and alleviated the damage to hemisphere with more visible neurons, compared with HIE group. Compared with sham group, the levels of autophagy-related proteins Atg5, beclin-1 and LC3-Ⅱ/-I in the cortex were increased (P<0.01), and P62 protein expression was decreased (P<0.01) in HIE group. Compared with HIE group, the protein expression of Atg5, beclin-1 and LC3-Ⅱ/-I was decreased (P<0.01) and P62 protein expression was increased in HIE+EA pretreatment group (P<0.01). In vitro, compared with CoCl2 group, the PC12 cells in CoCl2+EA pretreatment group showed a lower ROS level. Moreover, the cells in CoCl2+EA pretreatment group exhibited higher mitochondrial membrane potential than that in CoCl2 group. MDC staining in CoCl2 group showed high value of fluorescence and increased number of autophagosomes. EA pretreatment reduced the number of autophagosomes and the extent of autophagy to protect PC12 cells. Furthermore, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I in CoCl2 group were higher (P<0.01), and the protein expression of P62 was lower (P<0.01) than those in control group. In CoCl2+EA pretreatment group, the protein levels of Atg5, beclin-1 and LC3-Ⅱ/-I were decreased (P<0.01) and the protein expression of P62 was increased as compared with CoCl2 group (P<0.01). CONCLUSION:EA pretreatment attenuates autophagy to protect the neurons against HIE injury. 相似文献
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Pang Ze Li Ming-xue Zhou Jin-tao Qiu Zhen-dong Song Ying-ji Song Yan-ru Waqar Ahmed Song Bo Liu Shan-shan 《东北农业大学学报(英文版)》2021,28(1):1-14
Studies have shown that the three subunits of β-conglycinin are the main potential allergens of soybean sensitive patients.And β-conglycinin has adverse effects on nutrition and food processing.So solation and production of lines with lower β-conglycinin content has been the focus of recent soybean breeding projects.Soybean lines with deficiency in one or all subunits of β-conglycinin have been obtained.An effective and rapid system to identify such mutations will facilitate genetic manipulation of the β-conglycinin subunit composition.Here,two segregating F_2 populations were developed from crosses between Cgy-1/cgy-1 (CC),an α′-lacking line (Δα′),and DongNong 47 (DN47),a wild-type (Wt) Chinese soybean cultivar with normal globulin components,and Cgy-2/cgy-2 (CB),an α-lacking line (Δα),and DN47.These populations were used to estimate linkage among the cgy-1 (conferring α′-null) and cgy-2 (α-null) loci and simple sequence repeat (SSR) markers.Seven SSR markers (Sat_038,Satt243,Sat_307,Sat_109,Sat_231,Sat_108 and Sat_190) were determined to co-segregate with cgy-1,and six SSR markers (Satt650,Satt671,Sat_418,Sat_170,Satt292 and Sat_324) co-segregated with cgy-2.Linkage maps being composed of seven SSR markers and cgy-1 locus,and six SSR markers and the cgy-2 locus were then constructed.It assigned that the cgy-1 gene to chromosome 10 at a position between Sat_307 and Sat_231,and the cgy-2 gene to chromosome 20 at a position between Satt650 and Satt671.These markers should enable map-based cloning of the cgy-1 and cgy-2 genes.For different subunit-deficiency types[α′-null,α-null and (α′+α)-null types],the two sets of SSR markers could also detect of polymorphism between three normal cultivars and seven related mutant lines.The identification of these markers is great significance to the molecular marker-assisted breeding of soybean β-conglycinin subunits. 相似文献