沿渡河流域分布式水文模型的构建

按照综合分布式概念性水文模型和分布式水文物理模型长处的建模思路，应用改进的格林-安普特下渗理论和地貌水文学的水滴运动理论，建立了一个具有物理基础的松散结构分布式流域水文模型。模型的产流方法融合了邓恩产流理论和霍顿超渗产流理论，考虑土壤水分剖面、土壤各向异性和地形坡度等对流域产流的影响；模型的汇流方法利用地貌水文学理论，寻求水文过程与流域地形地貌的相互作用及定量关系，以确定模型参数，其优势在于可以考虑净雨空间分布不均和流速空间分不均对流域汇流的影响问题。以湿润地区典型流域沿渡河为对象进行水文模拟应用研究，结果良好,说明模型较为可靠。     

我国草地贪夜蛾应急化学防控风险评估及对策

草地贪夜蛾Spodoptera frugiperda (J.E.Smith)是世界性重大农业迁飞害虫, 在我国已呈严重暴发态势, 对我国玉米等农作物生产安全构成严重威胁?农药防治是草地贪夜蛾应急防控中最有效的手段之一, 但是不合理的使用农药会对环境安全和施药人员带来潜在的风险?本文结合我国田间化学农药防治草地贪夜蛾的实践和效果, 评估了8种田间常用防治药剂的环境风险及其对施药人员的健康风险?推荐甲氨基阿维菌素苯甲酸盐?乙基多杀菌素?氯虫苯甲酰胺?虱螨脲作为草地贪夜蛾应急防控的首推农药, 甘蓝夜蛾核型多角体病毒作为首推生物药剂, 高效氯氟氰菊酯和乙酰甲胺磷须谨慎使用?本研究从农药应用风险角度提出了具体的草地贪夜蛾合理用药建议, 以期为草地贪夜蛾的有效安全防控提供科学依据?     

Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism

This study was conducted to investigate the effects of alternating high temperature on Cry1Ac protein content on Bt cotton cultivars Sikang 1(SK-1,a conventional cultivar)and Sikang 3(SK-3,a hybrid cultivar).In 2011 and 2012,cotton plants were subjected to high temperature treatments ranging from 32 to 40℃ in climate chambers to investigate the effects of high temperature on boll shell insecticidal protein expression.The experiments showed that significant decline of the boll shell insecticidal protein was detected at temperatures higher than 38℃ after 24 h.Based on the results,the cotton plants were treated with the threshold temperature of 38℃ from 6:00 a.m.to 6:00 p.m.followed by a normal temperature of 27℃ during the remaining night hours(DH/NN)in 2012 and 2013.These treatments were conducted at peak boll growth stage for both cultivars in study periods of 0,4,7,and 10 d.Temperature treatment of 32℃ from 6:00 a.m.to 6:00 p.m.and 27℃ in the remaining hours was set as control.The results showed that,compared with the control,after the DH/NN stress treatment applied for 7 d,the boll shell Cry1Ac protein content level was significantly decreased by 19.1 and 17.5% for SK-1 and by 15.3 and 13.7% for SK-3 in 2012 and 2013,respectively.Further analysis of nitrogen metabolic physiology under DH/NN showed that the soluble protein content and the glutamic pyruvic transaminase(GPT)activities decreased slightly after 4 d,and then decreased sharply after 7 d.The free amino acid content and the protease content increased sharply after 7 d.The changes in SK-1 were greater than those in SK-3.These results suggest that under DH/NN stress,boll shell Cry1Ac protein content decline was delayed.Reduced protein synthesis and increased protein degradation in the boll shell decreased protein content,including Bt protein,which may reduce resistance to the cotton bollworm.     

转基因抗虫作物中豇豆胰蛋白酶抑制剂（CpTI）酶联免疫检测方法的建立

 从10个不同豇豆品种中筛选出抑制剂活性最高的901青皮豇豆，提取豇豆胰蛋白酶抑制剂。通过G-75凝胶过滤、亲和层析纯化了豇豆胰蛋白酶抑制剂（CpTI）并制备了其兔多克隆抗体。用所制备的抗体建立了酶联免疫检测方法并检测了转CpTI编码基因棉花、水稻中的CpTI含量，发现棉花不同器官CpTI的表达量不尽相同。     

用凝胶电泳法研究小麦叶片衰老期间的内肽酶同工酶

采用SDS－聚丙烯酰胺凝胶电泳（SDS－PAGE）和梯度凝胶电泳方法，研究了小麦旗叶暗诱导衰老过程中内肽酶同工酶的变化。发现用SDS－PAGE法几科检测不到新的内肽酶同工酶；用梯度凝胶电泳法则能检测到6种新的同工酶，而且在第一叶自然衰老过程中也能检测到。表明在这两种衰老过程中内肽酶酶谱变化基本相似。     

水分对大豆蛋白质物料微波膨化的影响

就水分含量对大豆蛋白质物料微波膨化效果的影响进行了研究 ,认为含水量为 30 %时 ,较适宜利用微波进行膨化 ;水分影响膨化的机理在于决定膨化动力和微波能量提供的大小 ,同时也会影响蛋白质物料的凝胶结构及变性性质 ,从而最终影响微波膨化     

miR-204 inhibits proliferation of Hodgkin lymphoma cells by targeting Sirt1/p53 signaling pathway

AIM: To investigate the effect of microRNA-204 (miR-204) on the proliferation of Hodgkin lymphoma cells and the underlying mechanism. METHODS: The expression of miR-204 and Sirt1 mRNA in Hodgkin lymphoma tissues was detected by RT-qPCR. After transfection with miR-204 mimic, Sirt1 siRNA and miR-204 mimic+pcDNA3.1-Sirt1 into the L428 cells, the cell viability and BrdU incorporation were measured by CCK-8 assay and BrdU assay, respectively. The protein levels of Sirt1 and acetylated p53 (ac-p53) were determined by Western blot.The targeting relationship between miR-204 and Sirt1 was verified by double luciferase reporter assay. RESULTS: The low expression of miR-204 and the high mRNA expression of Sirt1 were found in the Hodgkin lymphoma tissues. Compared with control group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were significantly decreased after L428 cells were transfected with miR-204 mimic or Sirt1 siRNA (P<0.05). Compared with miR-204 mimic alone group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were increased after L428 cells were co-transfected with miR-204 mimic and pcDNA3.1-Sirt1 (P<0.05). The results of double luciferase reporter assay confiermed that Sirt1 was the target gene of miR-204. CONCLUSION: The inhibitory effect of miR-204 on the proliferation of L428 cells may be achieved by inhibiting the expression of Sirt1 and promoting the up-regulation of ac-p53.     

猪冷冻精子的体外受精与单精子胞浆内注射

用猪冷冻精子进行体外受精和单精子胞浆内注射(ICSI),并研究添加L-半胱氨酸及不同成熟培养法对猪ICSI胚胎发育的影响。结果表明:用猪冷冻精子生产的ICSI胚胎卵裂率及囊胚发育率分别为68.3%和11.4%,均低于用冷冻精子进行体外受精(IVF)所获得的卵裂率和囊胚发育率(分别为76.9%和19.2%,P<0.05)。在培养液中添加1.71mmol·L~(-1) L-半胱氨酸培养3 h,无论是卵裂率、桑椹胚发育率,还是囊胚发育率,均未得到明显改善(P>0.05);采用含0.4%BSA的NCSU-23培养液进行猪卵母细胞成熟培养,ICSI胚胎卵裂率虽明显高,但各组所获胚胎发育率均无显著差异(P>0.05),表明所用卵母细胞的成熟方法并未对ICSI胚胎发育产生明显的影响;用颗粒细胞或卵丘细胞共培养ICSI胚胎后,卵裂率有明显提高(P<0.05),但各组囊胚发育率无统计学差异。     

猪卵泡闭锁过程中颗粒细胞凋亡和Fas/FasL变化

为了揭示猪卵巢卵泡选择性闭锁机制,采用Real time PCR和HE染色方法,检测猪各阶段卵泡颗粒细胞中Fas和FasLmRNA表达和形态学变化;分析比较不同发育阶段卵泡中颗粒细胞Fas和FasLmRNA表达差异及细胞凋亡程度。结果表明,健康的大(5 mm)、中(3～5 mm)、小(3 mm)卵泡,早期闭锁的大、中、小卵泡以及晚期闭锁的大、中、小卵泡壁层颗粒细胞都有Fas和FasLmRNA表达。早期闭锁卵泡颗粒细胞Fas和FasLmRNA相对表达量高于健康卵泡与晚期闭锁卵泡。早期闭锁小卵泡FasLmRNA相对表达量与大、中、小健康卵泡相比均有显著差异(P0.05);状态相同的大、中、小卵泡间Fas与FasLmRNA相对表达量均无显著差异(P0.05);表明Fas/FasL系统在猪卵巢卵泡选择性闭锁过程中,对颗粒细胞凋亡具有重要的调节作用。     

生防菌FM4B的鉴定及抗菌物质的性质研究

采用生理生化、16S rDNA等方法鉴定菌株FM4B并对该菌株抑菌物质粗提液的性质进行了初步研究.生理生化实验显示,菌株FM4B属于短短芽孢杆菌,16S rDNA基因的测定与分析表明,FM4B与B.brevis(短短芽孢杆菌)的同源性达到99.5%,故推定FM4B为短短芽孢杆菌.实验表明抑菌物质粗提物对多种细菌、真菌有...