利用大白菜DH群体构建AFLP遗传连锁图谱

 以大白菜‘汴早- 26’和‘光90E16’的F₁ 代进行游离小孢子培养所获得的含有59个株系的DH群体为试材, 利用AFLP技术通过63对引物筛选共获得346个AFLP多态性标记, 运用JoinMap 3.0软件构建大白菜遗传连锁图谱。该图谱主要包括10个连锁群, 总图距为708 cM, 平均图距为2.0 cM。     

花椰菜游离小孢子培养再生植株研究

花椰菜 (BrassicaoleraceaL .var.botrytisL .)起源于欧洲地中海东部沿岸 ,属十字花科芸薹属植物 ,1 9世纪中叶传入我国。花椰菜质地洁白娇嫩 ,营养丰富 ,风味鲜美 ,现在我国各地普遍栽培。利用游离小孢子培养技术可以快速地获得纯合系 ,在蔬菜杂交育种中具有广阔的应用前景 ,已成为当前生物技术育种的重要方法〔1～4〕。但花椰菜游离小孢子培养技术目前仍不成熟 ,国内尚未见这方面的详细报道〔1,5～ 7〕。本试验以 1 2种花椰菜基因型为试材 ,进行游离小孢子培养技术研究 ,成功地诱导出了胚状体 ,并获得再生植株。1　材料与方法1 .1　材料…     

Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene

The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB₁₂, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor.     

发酵酸羊乳冻干粉的生产方法

介绍了发酵酸羊乳冻干粉的生产方法,主要有羊乳预处理、羊乳发酵、冻干制粉和包装质检四个生产工序。应用此法生产的酸羊乳冻干粉保留了酸羊乳原有的色、香、味和营养物质,还具有较高的乳酸菌生物活性。     

农业科研单位青年科技人员思想政治工作初探

思想政治工作是党的建设工作的重要法宝，充分体现了党组织的统领全局作用和凝聚作用。随着农业体制机制改革的日益深入，农业科研单位正经历着复杂而又深刻的改革，同时，青年科技人员的思想也在悄然变化。农业科技创新归根到底取决于青年科技人员，面对青年科技工作者新的思想动态、精神状态的变化，必须开展有效的思想政治工作，以帮助青年科技人员树立正确的思想信念，克服错误的思想倾向，为深入推进农业科研单位健康、协调发展提供强大的精神正能量。     

筹建中的研究所如何进行制度建设

从筹建中研究所制度建设的必要性、应遵循的原则以及如何进行给予阐述,建议筹建中的研究所进行制度建设时应明确单位宗旨与目标;明确“立法”部门、成立专项工作小组;拟订制度建设方案;构建制度框架体系;注重制度之间的衔接贯通以及重点抓好制度的执行等。     

Design method and homogeneity control of bridge fair-faced concrete

According to the structural characteristics of bridge engineering, the mix optimization design method of bridge fair-faced concrete was presented, and C30~C50 concrete with high cohesion and package, outstanding fluidity and excellent mechanical properties were prepared. Through the test of air content of fresh concrete, micro hardness and micro structure in different parts of hardened concrete specimens, effects of viscosity-modifying admixtures on homogeneity of concrete were discussed. The results showed that the composition of bridge fair-faced concrete were relatively homogeneous, and the microstructure were relatively dense. When C30 concrete mixed with 5 × 10 -5 cellulose ether or 6% silica fume (mass fraction of cementitious material), air content of fresh concrete were less than 2%, and slurry viscosity values were between 1 900 and 2 000 MPa·s. Also, the micro hardness values in different parts of hardened concrete specimens were closed. The results were applied in practical bridge engineering, which generally meets the apparent quality requirements of bridge fair faced concrete.     

早籼型水稻温敏核不育系M103S的选育与光温反应特性

以温敏核不育水稻安农S—1为母本，以常规高产早籼稻品系92153为父本进行杂交，通过低温选择和人工气候箱鉴定，育成了育性转换临界温度23—24℃的早籼型温敏核不育系M103S。光周期长于12．5h，日平均温度高于24℃，可以用于制种，日平均温度低于23℃，可以用于繁殖。光周期长于13．5h，日平均温度高于23℃，可以用于制种。自然条件下不育起点温度为日均温23—24℃，耐低温期为3—4d。敏感期为抽穗前的7—14d。播始历期为53—79d，主茎叶片数10．0—12．6片。从播种到始穗的活动积温和有效积温，分别为1493．8—1766．3℃和733．4—1058．3℃。不育期的株高63．6—77．5cm，平均72．0cm，单株有效穗6．4—12．0个，平均9．3个，穗长20．9—22．7cm，平均21．2cm，每穗颖花数112．1—137．3朵，平均122．3朵。可育期株高65．7cm，单株有效穗7．2个，穗长17．2cm，每穗总粒数114．1粒，结实率47．23％，千粒重31．34g。以M103S为母本，与100多个早籼品种进行测配，杂种表现生育期早、穗大、粒多、千粒重高。     

速生人工林桉树木材材色处理初步研究

试验用漂白剂、酸性染料和其他化学药剂,采用单纯漂白、单纯染色、先漂白再染色和化学着色等4种不同的方案对人工林桉树木材进行材色改良处理研究.结果发现:单纯漂白可获得一定的材色改良效果;硫化物类、过硫酸铵类的药剂配方漂白效果不明显;硼氢化钠类的药剂配方可得到一定的漂白效果;双氧水类、次氯酸钠类的药剂配方可得到很好的漂白效果.单纯染色比较难获得好的材色处理效果.采用先漂白后染色的方法,获得的材色过于鲜艳.采用化学着色的方法,可获得较理想的材色改良效果;草酸类药剂配方着色效果明显,可消除材色缺陷,根据不同的配方和处理工艺可获得从浅粉红色到红棕色的色调;用双氧水做活性剂,引入带发色团的化学药剂,材色改良效果明显,通过调整配方药品的浓度和处理工艺可获得从浅黄色到橙黄色的不同色调和较佳的材质.     

Identification of new strawberry sulfur volatiles and changes during maturation

Two Florida strawberry cultivars, 'Strawberry Festival' and 'Florida Radiance', were harvested at five fruit developmental stages (white, half red, three-quarter red, full ripe, and overripe) at four harvest dates. A static headspace solid-phase microextraction (SPME) sampling technique coupled with gas chromatography (GC) using pulsed flame photometric detection (PFPD) was employed to measure 16 sulfur volatiles in these strawberries. A total of 7 sulfur volatiles have been previously reported, and 9 are reported for the first time in strawberries. Newly identified sulfur volatiles include methyl thiopropionate, ethyl thiobutanoate, methyl thiohexanoate, methyl (methylthio)acetate, ethyl (methylthio)acetate, methyl 2-(methylthio)butyrate, methyl 3-(methylthio)propionate, ethyl 3-(methylthio)propionate, and methyl thiooctanoate. Identifications were based on matching sulfur peak linear retention indexes (LRIs) of unknowns with authentic standards and gas chromatography-mass spectrometry (GC-MS) data. Concentrations were determined using both internal and external standards. Most sulfur volatiles increased with increasing maturity, with only concentrations of hydrogen sulfide and methanethiol remaining relatively consistent at all five stages. At the white and half red stages, most sulfur volatiles consisted of various alkyl sulfides. At three-quarter red (commercial ripe), full ripe, and overripe stages, the majority of sulfur volatiles consisted of sulfur esters. Most sulfur volatiles increased dramatically between the commercial ripe, full ripe, and overripe stages, increasing as much as 100% between full ripe and overripe. Principal component analysis indicated that sulfur volatiles could be used to distinguish overripe from full ripe and commercial ripe berries.