Effects of music stimulus on behavior response,cortisol level,and horizontal immunity of growing pigs

An enriched environment is widely used to improve domestic animals’ welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs.     

5种生物制剂对马铃薯种薯萌芽、极端天气下植株生长和产量性状的影响

旨在满足马铃薯生产中茬口衔接、机械化生产技术应用、不利气候下稳产等对马铃薯出苗早、齐、壮的需求,以‘费乌瑞它’为供试品种,用基于有益活菌或工程菌提取物的5种生物制剂进行种薯处理,对多重性状进行了对比分析。5种生物制剂较常规化学制剂,均能够不同程度地促进种薯萌芽和芽根同生,出苗期提前2~7天,播种后49天的出苗率提高3.33%~17.78%。其中,表现最好的为酵母核苷酸衍生物和VDAL,种薯萌发和生根均显著高于对照。霜冻后,生物剂拌种处理在恢复前期促进植株生长,由此促进恢复后期的块茎发育,较常规化学处理增产8.39%~24.03%,体现了不同程度的保产效果。多马道黑、酵母核苷酸衍生物、根肽和VDAL体现出较好的保产效果,可作为种薯处理剂投入马铃薯生产。     

特早熟抗病棉花品种辽棉35选育及栽培技术

本文主要介绍辽棉35的选育过程、生物学特性、产量、纤维品质、抗病性及栽培技术要点。     

4个甜樱桃品种在德州的引种表现及设施栽培技术

对引入山东德州的4个甜樱桃品种美早、萨米脱、布鲁克斯、拉宾斯进行了日光温室栽培试验,对其性状表现进行了观察。结果表明,4个品种均表现生长良好,适应性强,结果正常,果实品质优良,可以作为德州地区日光温室的栽培品种发展。简述了4个甜樱桃品种在日光温室的栽培技术。     

利用SOM聚类分析及主成分分析方法鉴定小麦品种萌发期耐盐性

为了研究不同小麦品种萌发期的耐盐性,为小麦萌发期耐盐性鉴定提供快速、准确的鉴定方法和理论依据。本研究以40个冬小麦品种为试验材料,通过主成分分析法(PCA)与神经网络自组织映射(SOM)聚类分析法,研究盐胁迫对40个小麦品种萌发期各项形态指标的影响。主成分分析法结果表明,发芽势、发芽率、最长根长、第一片叶长及胚芽鞘长在PCA下载荷量最大;根据SOM聚类分析结果,‘德抗961’等4个小麦品种属于高度耐盐品种,‘济麦22’等14个小麦品种属于耐盐性品种,‘济麦20’等16个品种属于中等耐盐性品种,‘鲁麦21’等6个小麦品种属于盐敏感性品种。发芽势、发芽率、最长根长、第一片叶长、胚芽鞘长能够作为萌发期小麦耐盐性筛选的主要评价指标,并且结合主成分、SOM聚类分析方法进行小麦萌发期耐盐性鉴定更为科学、方便。     

Three SNPs within exons of INHA and ACVR2B genes are significantly associated with litter size in Dazu black goats

The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary.     

基于遥感指数的草原火后植被恢复研究

草原火是草原生态系统重要的干扰因子，严重影响着系统的结构与功能。基于遥感数据，以2015年“4·16”特大草原火灾为例，利用ENVI和ArcGIS等软件，分别对NDVI和GPP指数及火烧严重度下的植被恢复过程进行定量分析。结果表明，基于NDVI和GPP指数的火后植被恢复过程表现相似，不同年份植被恢复情况存在一定差异。火灾发生当年（2015年）火烧迹地植被恢复状况高于未发生火灾区域，而在火后第1年（2016年）却又稍低于未发生火灾区域，直到火后第2年（2017年），火烧迹地植被基本恢复到火前状态。同时，不同火烧严重度下的植被恢复过程在存在明显差异。草原火灾发生后当年（2015年），中强度火烧下植被恢复最好，其次是轻度，重度表现最差。草原火烧严重度对植被恢复的影响，主要表现在火后第1个植被生长季。在之后的年份里，由于草原更新能力强大，不同火烧严重度对草原植被的影响不明显。本研究可丰富草原火灾及火烧迹地植被恢复等相关研究成果，同时为草原火灾管理和草原生态系统的可持续发展提供科学依据。     

电超滤浸提土壤养分的研究 Ⅰ.石灰性土壤的电超滤特性

石灰性土壤的电超滤(EUF)特性主要是浸提时因需控制电流而必须大大降低电压。在一定范围内,降压程度与土壤 CaCO_3%有密切关系。控流限额愈低,电压需成比例地下降,但土壤EUF-值减小不多。电流控制在一定值时,离子的解吸总量亦为定值。控流主要控制了各石灰性土壤解吸/溶解份额特大的 Ca+Mg 量近于定值,对于含量小的其他养分离子则并无多大影响,仍可区辨各土壤间的差别。通过冬小麦盆栽试验和有关化学测试法等方面的相关研究,表明采用国际通用的5g 土样和控电流方法测定 EUF-K 和-P 等有效养分,对于石灰性土壤也是可行的,但其EUF-(Ca+Mg)值则毫无植物营养有效性的意义。土壤 EUF 值只是一种指数,并非真正的解吸离子量。石灰性土壤 EUF-K 值因浸提时降压控流而略低,80℃时5min 内解吸的钾不一定是非交换性钾。由于 Ca-P 和 CaCO_3在高温高电压时浸出较多,故石灰性土壤 EUF-P 和-(Ca+Mg)的80℃值/20℃值之比,与酸性土壤或非石灰性土壤相比要大得多。     

茉莉酸对苹果气孔运动及抗旱性的影响

50、75、100、150 mg／L JA处理苹果叶片表皮，使开放气孔的气孔开度分别减小27．4％、37．5％、56．9％和63．8％，使关闭后的气孔在光下开放时气孔开度分别减小15％、18％、28．8％、52．5％、61．3％；处理植株后干旱胁迫，叶片相对电导率分别降低15％、18％、31％、58％，脯氨酸含量分别提高0．09％、0．40％、0．42％、0．54 ng／gFW，可溶性糖含量分别提高1．89％、2．53％、3．80％、5．06％。25 mg／L JA处理与对照均无显著差别。JA的作用表现出明显的浓度效应。     

木质材料的表面劣化与木材保护的研究

本试验以枫桦(Betula costata Trautv)、紫椴(Tilia amurensis Rupr)木材单板和凸版纸、新闻纸、条纹牛皮纸、书写纸、胶版纸和朴克原纸为试材,借助于电子自旋共振波谱仪和扫描电子显微镜观测和分析了经室内、外暴露和人造紫外光辐射期间各种木质材料表面化学和表面性状的变化;提出了抑制木材表面劣化,保护木材的处理方法。研究结果表明,采用电子自旋共振波谱仪和扫描电子显微镜可以监测木质材料的劣化过程。采用化学药剂处理和油漆涂饰处理均可抑制紫外光对木材表面的光化降解,其中用浓度为5％的三氧化铬溶液或三氯化铁溶液处理木材其保护作用显著,能够改善木材的耐候性能。