Evaluating eddy covariance method by large-scale weighing lysimeter in a maize field of northwest China

Accurate determination of evapotranspiration (ET) is useful to develop precise irrigation scheduling. Although eddy covariance (EC) is a direct method which is widely used to measure ET, its performance in arid region of northwest China is not clear. In this study, ET measured by EC (ET_EC) was compared with that by large-scale weighing lysimeter (ET_L) during the whole growing season of maize in 2009. Energy balance ratio was 0.84 for daytime fluxes, indicating that lack of energy balance closure occurred, so daytime ET_EC was adjusted by Bowen-ratio forced closure method. Compared to the corresponding ET_L, half-hourly daytime ET_EC was underestimated by 21.8% without the adjustment and 4.8% with the adjustment. Furthermore, nighttime ET_EC was adjusted using filtering/interpolation method. Mean error between half-hourly nighttime ET_EC and ET_L decreased from 30.2% without the adjustment to 10.3% with the adjustment. After such adjustment of day and night measurements, daily ET_EC was underestimated by 6.2% compared to ET_L. These results indicated that the adjusted ET_EC well matched with the ET_L. Moreover, the discrepancy of adjusted total ET_EC and ET_L was decreased to 3.2% after subtracting the overestimated ET by lysimeter resulting from irrigation and heavy rainfall events. Thus, after appropriate adjustments of observations, eddy covariance method is accurate in estimating maize ET in the arid region of northwest China.     

Effect of water deficit in different growth stages on stem sap flux of greenhouse grown pear-jujube tree

The effects of water deficit in different fruit growth stages on the variation of stem sap flux of 6-year old greenhouse-grown pear-jujube trees were investigated. Treatments included sufficient water supply during the whole fruit-growing period (T₁), mild water deficit during the flowering–fruit setting stage (T₂), moderate water deficit during the fruit rapid growth stage (T₃) and severe water deficit during the fruit maturing stage (T₄). Results showed that significant compensation effect on stem sap flux after re-watering was observed in T₂, but not in T₃ and T₄ stages. At the end of rapid growth stage, the diurnal variation of stomatal conductance generally had a similar trend as that of stem sap flux, but with a distinct midday depression from 12:00 to 14:00 p.m. In addition, a linear relationship between the relative available soil water content (RAWC) and the ratio of daily stem sap flux to that of sufficient water treatment was observed (R² = 0.4489).     

湖南省三道坑自然保护区生态旅游资源评价

简述了生态旅游与自然保护区的关系,确定三道坑自然保护区具有丰富、珍稀的植物、动物资源.有优美多样的人文景观资源.应用通行方法对生态旅游资源进行了定量与定性评价:自然保护区主要旅游资源23处,有优良旅游资源5处,普通旅游资源18处;自然保护区内生态环境良好,不同的旅游资源组合具有补充性、旅游资源质量一般、总体开发前景较好...     

Recombinant Expression of a Modified Shrimp Anti-Lipopolysaccharide Factor Gene in Pichia pastoris GS115 and Its Characteristic Analysis

Anti-lipopolysaccharide factors (ALFs) with a LPS-binding domain (LBD) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. FcALF2 was one isoform of ALFs isolated from the Chinese shrimp Fenneropenaeus chinensis. Our previous study showed that a modified LBD domain (named LBDv) of FcALF2 exhibited a highly enhanced antimicrobial activity. In the present study, a modified FcALF2 gene (mFcALF2), in which the LBD was substituted by LBDv, was designed and synthesized. This gene was successfully expressed in yeast Pichia pastoris GS115 eukaryotic expression system, and the characteristics of the recombinant protein mFcALF2 were analyzed. mFcALF2 exhibited apparent antibacterial activities against Gram-negative bacteria, including Escherichia coli, Vibrio alginolyticus, Vibrio harveyi, and Vibrio parahaemolyticus, and Gram-positive bacteria, including Bacillus licheniformis and Staphylococcus epidermidis. In addition, mFcALF2 could reduce the propagation of white spot syndrome virus (WSSV) in vivo by pre-incubation with virus. The present study paves the way for developing antimicrobial drugs in aquaculture.     

关于季节性冻土对兴隆林区的危害及防治措施

本文论述了在严寒地区预防和减轻基础冻害的方法及措施,并在实践中得到证实。     

Variability in energy partitioning and resistance parameters for a vineyard in northwest China

Grapevines are extensively grown in the semiarid and arid regions, but little information is available on the variability of energy partitioning and resistance parameters for the vineyard. To address this question, an eddy covariance system was applied to measure energy balance over a vineyard in northwest China during 2005-2006. Result indicated that 2-year average Bowen ratio (β) of vineyard was 1.0, canopy resistance (r_c) 289.3 s m⁻¹, aerodynamic resistance (r_a) 9.7 s m⁻¹ and climatological resistance (r_i) 117 s m⁻¹. This implied that the annual energy was split almost equally between sensible heat and latent heat. Compared to the corresponding values in other ecosystems reported by Wilson et al. [Wilson, K.B., Baldocchi, D.D., Aubinet, M., Berbigier, P., Bernhofer, C., Dolman, H., Falge, E., Field, C., Goldstein, A., Granier, A., Grelle, A., Halldor, T., Hollinger, D., Katul, G., Law, B.E., Lindroth, A., Meyers, T., Moncrieff, J., Monson, R., Oechel, W., Tenhunen, J., Valentini, R., Verma, S., Vesala, T., Wofsy, S., 2002. Energy partitioning between latent and sensible heat flux during the warm season at FLUXNET sites. Water Resource Research 38, 1294-1305.], the vineyard had a higher β, r_c and r_i than deciduous forests, corn and soybean, and grassland. Such difference was mainly attributed to (1) serious water stress in 2005, which resulted in a greater r_c up to 364.4 s m⁻¹; (2) sparse canopy with row spacing of 2.9 m and plant spacing of 1.8 m; (3) warm-dry climate and high attitude (1581 m) along with higher r_i and lower psychrometer (54 Pa K⁻¹) in the arid region of northwest China. These characters of vineyard revealed varying process of energy partitioning and surface resistance, and provided a scientific basis in understanding and modeling water and energy balance for the vineyard in the semiarid and arid regions.     

安哥拉山羊级进杂交后代生活力初步观察

对安哥拉山羊及其级进杂交后代的生长发育、繁殖力、抗病性、采食习性等进行初步观察研究,结果表明：在试验场良好的饲养条件下,各代杂种羊生长发育正常,但繁殖力、抗病性、采食能力随级进杂交代数的增加有降低的趋势。据此,笔者认为,在陕北特定的生态经济条件下,培育毛用山羊新品种,级进杂交代数不宜太高,到Ｆ３代应停止级进,进行横交固定,而且在整个育种中,应始终注意饲养问题,以保证新品种培育成功。     

割手密线粒体DNA的简易快速提取方法

高效快速地分离提取高质量的线粒体DNA(mtDNA)是研究植物线粒体基因及其起源进化的重要前提。为获得高质量的mtDNA进行割手密资源的线粒体基因序列扩增及测序分析,本研究以割手密黄化苗为材料,通过简单差速离心分离得到线粒体,经DNaseⅠ消化处理,去除核DNA杂质得到较纯的线粒体,然后经过5%SDS和蛋白酶K充分裂解线粒体,利用饱和的苯酚/氯仿(1:1)和氯仿两次抽提除去蛋白质,并经过RNase A的消化处理除去RNA,无水乙醇沉淀等一系列操作得到mtDNA。所提取的mtDNA样品经紫外吸收光度测定,琼脂糖凝胶电泳和PCR扩增分析其浓度和纯度,结果表明利用该方法提取的mtDNA纯度高,完全能满足后续PCR分析及分子克隆测序的要求。该方法不仅DNA提取纯度高,操作简单、快速经济,可为今后开展甘蔗及其各野生种的研究提供技术支持。