轻型木结构建筑覆板的集中静载性能

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

ASP技术在"中国茶主题网关"建设中的应用

介绍了网站信息资源组织的新模式--主题网关的概念与特征;介绍了ASP技术的相关概念;并以中国茶主题网关建设为例,介绍了ASP技术在主题网关建设中的应用.     

苦竹各器官主要营养元素分布及采伐的养分输出

本文根据四川长宁苦竹(Pleioblastus amarus)各器官生物量以及其主要营养元素含量和储量,研究了苦竹采伐的养分输出问题。结果表明:苦竹各器官中N、P、K、Si、Ca、Mg等6种营养元素以代谢旺盛的竹叶和竹鞭中含量最高;6种养分元素在各器官贮存量(单位:kg.hm-2)从大至小的排序为:叶(510.8)>竹根(230.62)>杆(182.14)>枝(163.77>竹鞭(59.19)>竹蔸(41.16)。苦竹采伐的养分年输出量(单位:kg.hm-2)从大至小的排序为:Si(50.23)>K(38.77)>N(35.58)>Mg(4.55)>P(3.86)>Ca(3.31)。对比该地区的降雨输入,苦竹林地养分以Si失衡最为严重;其次为K、N。若采伐时将竹叶带出竹林,6种元素的养分输出总量将增加1.48倍。可见,竹叶归还林地对维持地力具有极其重要的作用。     

干热河谷和干性森林水分特征的对比分析

本文研究了川西南山地安宁河流域干热河谷和干性森林的水分收支状况。结果表明:(1)干热河谷和干性森林的年降水量分别为1 067.95 mm和1 371.73 mm,年降水量的90%以上集中在6月～10月的雨季和夏秋季节;(2)干热河谷和干性森林年蒸发量为1 280 mm和1 089.75 mm,春夏蒸发占年蒸发量的65%和66%,旱季蒸发占年蒸发量的58%和61%,4月～6月是蒸发旺季,其次是7月～8月;(3)干热河谷和干性森林年水分盈余量分别为43.6 mm和481.95 mm,均存在季节性水分匮缺期,7月～10月、夏秋季节和雨季均是水分盈余期,其余为水分匮缺期;(4)干热河谷和干性森林年干燥度分别为1.205和0.8,表明该地区分别为半湿润和湿润性气候,还存在明显的季节差异,7月～10月、雨季和夏秋季节气候湿润,其余时期为半湿润或干旱气候;(5)干性森林在年降水量和水分盈余量上高于干热河谷,在年可能蒸发量和干燥度上明显低于干热河谷,这种差异还体现在水分参数的月分配、四季分配、旱雨季分配上;(6)干热河谷湿润程度差于干性森林,干热河谷植被和干性森林对各自水分收支状况具有良好的适应性;(7)封山育林需要注意旱季防火,人工促进还需要考虑土壤水分平衡规律和适地适树,进行"适度"造林。     

不同灌溉施肥方式的土壤硝态氮分布特性试验研究

通过大田测坑玉米种植试验研究,分析了常规畦灌和膜孔灌条件下农田土壤硝态氮的分布特性。结果表明:膜孔灌溉0~60 cm土层的硝态氮在作物的整个生育期变化较大,60 cm以下变化平缓,上下土层硝态氮含量差异相对较小。而常规畦灌在0~40 cm内变化较大,上下土层间硝态氮含量差异较大。两种灌溉方式,表层土壤的硝态氮含量在整个玉米生育期为双峰形式,以三叶期和抽穗期为最高。与常规畦灌相比,膜孔灌溉消除了硝态氮的表聚现象,土壤剖面硝态氮分布更趋合理,更加有利于作物的吸收利用。膜孔灌溉由于其覆膜作用,减轻了硝态氮的淋洗,提高了氮素的利用率。     

膜孔灌灌施条件下硝态氮迁移分布规律研究

利用自行研制的膜孔点源入渗装置,测试膜孔灌灌施条件下硝态氮的迁移和分布规律。结果表明:在肥液连续入渗过程中,硝态氮浓度锋运移与水分湿润锋是一致的;随着距离膜孔中心距离的增加,NO3-N含量减小,在湿润锋位置处土壤NO3-N含量急剧减小到本底值;进入再分布过程后,土壤水分运移速度减慢,整个湿润土体内的含水量分布更加均匀;再分布1~7天内,硝态氮含量略有下降,再分布10天后,硝态氮反硝化作用增强,径向处的反硝化作用弱于垂向处,硝态氮含量明显降低。     

玉米细菌性枯萎病菌的环介导恒温扩增(LAMP)检测方法

为了提高口岸和基层实验室检疫和监测玉米细菌性枯萎病菌的准确性和工作效率,利用环介导恒温扩增技术(LAMP),根据内切葡聚糖酶(EGase)基因前导序列,设计2个内引物和2个外引物,对玉米细菌性枯萎病菌进行快速检测。结果表明,使用玉米细菌性枯萎病菌的近缘种或引致相似症状的病原菌菊欧文氏菌玉米致病变种Erwinia chrysanthemi pv.zeae、玉米内州萎蔫病菌Clavibacter michiganensis subsp.nebraskensis、燕麦假单胞菌Pseudomonas avenae、杓兰欧文氏菌Erwinia cypripedii检测其特异性,仅玉米细菌性枯萎病菌有扩增。LAMP检测灵敏度达到2 pg DNA,为普通PCR的100倍;与其它检测方法相比,LAMP方法检测时间短,效率高,不仅降低了设备投入,易于操作,而且具有较高的灵敏度和特异性,适合玉米细菌性枯萎病菌的现场检疫和大规模检测。     

灌水定额对波涌灌溉土壤间歇入渗特性的影响

通过肥液(KNO3溶液)室内入渗试验,模拟研究了地下水位埋深150 cm条件下灌水定额对波涌灌溉土壤间歇入渗特性的影响.结果表明,在试验条件下平均入渗速度随灌水定额的增加而减小;灌水定额越大平均周期减渗率越大;土壤含水率增幅随灌水定额的增加而增大;灌水定额越大,土壤水分向下运移越深,进入地下水中的水量越多,越容易造成地下水污染和水资源浪费.