全文获取类型
收费全文 | 22046篇 |
免费 | 1308篇 |
国内免费 | 2360篇 |
专业分类
林业 | 1837篇 |
农学 | 2135篇 |
基础科学 | 1290篇 |
2481篇 | |
综合类 | 9225篇 |
农作物 | 1657篇 |
水产渔业 | 968篇 |
畜牧兽医 | 3826篇 |
园艺 | 1175篇 |
植物保护 | 1120篇 |
出版年
2024年 | 135篇 |
2023年 | 395篇 |
2022年 | 912篇 |
2021年 | 984篇 |
2020年 | 970篇 |
2019年 | 928篇 |
2018年 | 615篇 |
2017年 | 952篇 |
2016年 | 697篇 |
2015年 | 1013篇 |
2014年 | 1096篇 |
2013年 | 1252篇 |
2012年 | 1642篇 |
2011年 | 1771篇 |
2010年 | 1639篇 |
2009年 | 1406篇 |
2008年 | 1383篇 |
2007年 | 1157篇 |
2006年 | 1030篇 |
2005年 | 911篇 |
2004年 | 650篇 |
2003年 | 609篇 |
2002年 | 854篇 |
2001年 | 731篇 |
2000年 | 545篇 |
1999年 | 320篇 |
1998年 | 180篇 |
1997年 | 137篇 |
1996年 | 125篇 |
1995年 | 110篇 |
1994年 | 73篇 |
1993年 | 88篇 |
1992年 | 99篇 |
1991年 | 81篇 |
1990年 | 64篇 |
1989年 | 48篇 |
1988年 | 31篇 |
1987年 | 22篇 |
1986年 | 11篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 7篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1962年 | 6篇 |
1956年 | 7篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
综述皱木耳Auricularia delicata新品种"鹿肚耳"的形态特征和食用特点,测定其与黑木耳、毛木耳、毛木耳白色变种"玉木耳"人工栽培子实体的基本营养成分、矿质元素含量和氨基酸组成,评价其营养价值。结果表明:鹿肚耳热量235 kJ/100g、蛋白质7.21 g/100g、脂肪1.5 g/100g、碳水化合物48.4 g/100g、总膳食纤维29.3g/100g,含有5种常量元素、5种必需微量元素,其中,硒元素含量是黑木耳的4.6倍;含有人体所需的8种必需氨基酸,氨基酸化学评分(CS)和氨基酸评分(ASS)分别为12和18.86,色氨酸为其限制性氨基酸;蛋白质综合评价低于其他3种木耳。 相似文献
2.
Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides. 相似文献
3.
4.
5.
6.
7.
花生品质性状的稳定性和基因型-环境互作研究,可为花生品质育种及不同生态区域花生品种的选择提供参考依据。本研究利用GGE-biplot工具,对我国黄淮海花生主产区16个花生品种两年间的品质性状进行了综合分析,包括含油量、油酸、亚油酸、棕榈酸和蛋白质含量等。结果显示,5个品质性状均有较高的GGE总变异值(主成分因素PC1和PC2变异的总和),变幅在61.5%-79.9%之间,以含油量的GGE变异值最低,为61.5%,油酸含量的GGE变异值最高,为79.9%。16个花生品种2年间部分品质性状表现较为一致,其中濮花9519的含油量表型值波动最小,山花9号的亚油酸含量表型值波动最小,开农49是油酸含量表型值最为稳定的品种,天府23号是棕榈酸含量和蛋白质含量表型值最为稳定的品种。初步明确了徐州和濮阳分别适合高油花生和高油酸花生种植,确定了不同生态类型试点下较适合种植的品种,为花生品种推广应用提供了参考。 相似文献
8.
“基础化学”理论和实验教学质量的“高与低”在一定程度上直接关乎高职院校与化学相近专业人才培养的“成与败”。以教学改革为切入点,在“基础化学”教材建设和系列微课的制作及应用2个维度上,探讨提升“基础化学”课程教学质量的具体措施。 相似文献
9.
10.
Improving green development efficiency is urgently required, yet challenging, since it comprehensively reflects the bidirectional evolutionary relationship between regional development and resource and environmental consumption. The issue of regional green development efficiency has become a key topic; however, where the increase in efficiency originates and its spatial spillover effect remains unclear to date. Therefore, the spatial spillover effect of the green development efficiency of Shandong Province was quantified through the slack variable (SBM)-Undesirable and Spatial Durbin model. The green development efficiency showed clear spatial differentiation in Shandong Province. This was more obvious than the polarized development trend of the provincial capital circle that has Jinan at its core and the eastern coastal region with Qingdao at its core. Green development efficiency has a significant spatial spillover effect. The regression coefficient of the direct effect of the economic development level is positive. The regression coefficient of the indirect effect of the marketization level and government financial support level is negative. Science and technology played a positive role in the promotion of the green development efficiency of local and neighboring cities. This study contributes empirical evidence to the green development efficiency associated with regional development and resource and environment consumption of Shandong Province. 相似文献