Alfalfa Phytoestrogen Content: Impact of Plant Maturity and Herbage Components

Legumes contain a range of non‐nutritional phytochemicals that may have health‐promoting effects in humans. In this study, we determined the concentrations of four phytoestrogens (coumestrol, apigenin, luteolin and quercetin) in field‐grown alfalfa (Medicago sativa L.). Differences between plants of different stages of maturity, between plant parts, and different canopy segments were assessed. The concentration of individual phytoestrogen in whole herbage varied between 15 and 225 μg g^?1 dry matter (DM) and was strongly affected by stage of maturity. Coumestrol and apigenin concentrations were highest at early vegetative stages, luteolin and quercetin at early vegetative and late flowering stages. All phytoestrogens were found in lowest concentrations at the early flowering stage (average 68 μg g^?1 DM); stage at which alfalfa is usually harvested when used as a forage source for animals. At vegetative stages, apigenin was the predominant phytoestrogen in herbage followed by coumestrol, the reverse being observed upon initiation of flowering; luteolin and quercetin were found at all stages in similarly lower concentrations. Concentrations of luteolin, quercetin and apigenin were 225, 410 and 690 % greater, respectively, in flowers than in leaves or stems; coumestrol concentration was similar between plant parts. In flowers and stems the predominant phytoestrogens were apigenin and quercetin, followed by coumestrol and luteolin. Similar concentrations (average 26 μg g^?1 DM) of each of the four phytoestrogens were found in leaves. Concentrations through the herbage canopy varied and were greatest at >60 cm from the soil surface for apigenin and coumestrol, but greatest at >60 and 0–20 cm for quercetin and at 0–20 cm for luteolin. The results suggest that if alfalfa is to be used as a source of phytoestrogens and is harvested for the production of herbal supplements or nutraceuticals, management will need to be adapted.     

槟榔红色素的抗氧化活性

测定槟榔红色素(areca red pigment,AP)对 DPPH·和·OH的清除能力、对脂质体过氧化的抑制能力、对Fe2+的络合能力,并测定其还原能力.结果表明:槟榔色素对DPPH·、·OH的清除能力较强:对脂质体过氧化的抑制能力很强,抑制率高达79.84%;与VE、没食子酸(gallic acid,GA)、BHT相比,槟榔色素的还原力、对Fe2+的络合能力较弱.说明槟榔红色素是较好的抗氧化剂.     

精梳棉卷成卷过程中起泡问题分析

介绍了成卷工艺对棉卷结构的影响以及棉卷成卷过程中起泡现象与准备工艺的关系.分析了棉卷成卷工艺过程中起泡过程和起泡原因,提出了防止起泡的措施.     

3种农药的微核效应研究

运用蚕豆根尖微核技术研究3种农药(甲胺磷、甲基硫菌灵和盐酸吗啉胍)在不同浓度下的诱变效应。结果表明,3种农药诱发蚕豆根尖细胞微核率与对照组相比均有不同程度的上升(P<0.05或P<0.01),农药浓度与微核率呈剂量-效应关系,盐酸吗啉胍的诱变效应大于其他2种农药。     

狗舌草提取物诱导淋巴细胞性白血病L1210细胞分化的研究

以单猪屎豆碱（MO）、槲皮素（QU）和环磷酰胺（CY）为参照，观察了狗舌草600mL／L乙醇提取物（EX）对淋巴细胞性白血病L1210细胞体外试验的形态变化；利用流式细胞术，从DNA分子水平上检查了EX对L1210细胞各周期相的影响，探讨EX对L1210细胞的分化机理。结果发现，EX能够使L1210细胞向淋巴细胞方向发展；经EX作用24h后，L1210细胞G0＋G1期的百分比较对照组明显升高。提示EX对L1210细胞增殖的抑制作用可能是由于G1期的阻滞所致。     

β-葡聚糖对啤酒易滤性影响的研究

通过对啤酒中β-葡聚糖的定性定量分析和啤酒易滤性的测定,发现溶解状态的β-葡聚糖对啤酒的过滤没有明显的影响,而β-葡聚糖凝胶对啤酒过滤有严重影响.啤酒中β-葡聚糖凝胶的分子量在300 000以上,当含量达到20 mg/1时,就会使啤酒膜过滤困难.啤酒的易滤性与β-葡聚糖含量之间里r=-0.222的不显著负相关,而与β-葡聚糖凝胶的含量之间呈r=-0.841~(***)的极显著负相关.     

Landscape and habitat factors affecting cabot's tragopan Tragopan caboti occurrence in habitat fragments.

Cabot's tragopan Tragopan caboti is an endemic and endangered pheasant of the lower montane forests of southeastern China. The typical habitats of the tragopan have been seriously fragmented because of forest management for timber production and farmland reclamation in recent years. The effects of the fragment size and isolation on the distribution of the cabot's tragopan were studied in Wuyanling Natural Reserve. Thirty one habitat fragments (2.5-48.5 ha) surrounded by non-habitat sapling coniferous forests, in an intensively managed forested landscape, were surveyed over four seasons for the occurrence of cabot's tragopan. Five of the 31 fragments were occupied in all four seasons and nine were not occupied. Both landscape and habitat factors affected the occurrence of cabot's tragopan, with landscape factors having the greatest effect. Large and less isolated habitat fragments containing a larger amount of the tree Daphniphyllum macropodum were occupied significantly more often than small, isolated fragments. The appearance of cabot's tragopan in the habitat fragments was best explained by the size of the fragments, the distance to the nearest suitable habitat and the amount of macropdous daphniphyllum trees. Our results could be used to improve the management of the forests where Cabot's tragopan occurs in southeastern China.     

黔西北山区野生兰花资源的分类及开发利用前景

文章通过对黔西北山区春兰、线叶春兰、春剑、蕙兰、送春、建兰、兔耳兰、多花兰、墨兰9个种类的野生兰花资源特征特性和栽培适应性的论述,提出了黔西北山区野生兰花资源的保存途径和开发利用前景.毕节地区从低海拔的赤水河流域到2000m以上的高海拔地区均有兰花生长,花色品种齐全,资源丰富.加强保护与合理开发利用现有野生兰花资源的前景十分广阔.     

番茄早疫病抗病性鉴定方法

 早疫病是番茄的主要病害,在我国南北方发生较严重,常给生产造成很大损失。进行抗病育种是解决早疫病的有效途径,此工作在国内已受重视,而抗病性鉴定方法,却是抗病育种的重要环节。