DCAB对荷斯坦奶牛泌乳性能、血液酸碱平衡及血清矿物质含量的影响

试验选用荷斯坦泌乳早期奶牛15头,采用3X3复拉丁方试验设计,研究日粮DCAB水平分别为+367、+514、+663mmol/kgDM对奶牛生产性能、血液酸碱平衡和血清矿物质含量的影响。试验结果,不同DCAB水平日粮对奶牛奶产量差异不显著(P>0.05),高DCAB水平日粮可增加奶牛血液标准HCO-3含量(P<0.05),不同DCAB水平对奶牛各种血清常量矿物质含量没有影响(P>0.05)。试验表明,日粮DCAB水平超过+514mmol/kgDM可对奶牛奶产量产生不利影响。     

苹果属无融合生殖SERKs基因家族的克隆和表达分析

本研究利用同源克隆法以苹果属平邑甜茶和杂种后代叶片为试验材料,克隆得到4个SERK基因家族片段,并分析了SERKs家族基因在平邑甜茶和杂种后代不同器官和组织中的表达情况。研究结果表明:分离获得的4个SERK基因家族片段的氨基酸序列与模式植物拟南芥、烟草等植物的同源性均在84%以上,同时与其他物种的氨基酸序列进行Blast比对都具有较高的同源性;RT-PCR定量分析结果表明SERK1、SERK2、SERK3、SERK4基因主要在生殖器官中表达。因此,推测SERKs基因在平邑甜茶和杂种后代生殖发育过程中起着重要的调控作用。本研究结果为进一步揭示无融合生殖的发生机理奠定了基础。     

大型组合式机械通风玉米穗储粮仓的设计

本研究针对大农户玉米穗储藏的实际情况，研制了大型组合式机械通风玉米穗储粮仓，并对其进行了装粮试验。结果表明：该型储粮仓机械性能稳定，结构组装方便，降水、防霉、防鼠效果显著，可有效降低粮食产后损失，保证粮食品质。 [关键词] 粮仓|玉米穗|机械通风     

On the Analysis of EMCS and Its Application in the Project of Shenzhen Underground

In this paper, the selection of type, function and technological requirements of EMCS for Shenzhen Underground has been introduced.Based on the investigation for EMCS's worldwide applications and the analysis of practice in Shenzhen Underground, the comparison and selection of PLC and DDC have been done completely.At the same time,the introduction of EMCS is organized by the center level, station level and the spot level.     

Relationship between the ultrasonographic findings of suspected superficial digital flexor tendon injury and the prevalence of subsequent severe superficial digital flexor tendon injuries in Thoroughbred horses: a retrospective study

The onset of severe injury to the superficial digital flexor tendon (SDFT) is extremely difficult to predict from slight changes in ultrasonographic findings in cases with no apparent clinical signs. This study investigated the relationship between an increased cross-sectional area (CSA) or edema in the subcutaneous tissue around the tendon and the subsequent onset of severe SDFT injury in Thoroughbred racehorses. Horses were classified into three groups based on ultrasound diagnosis (USD) findings: Group A included cases with enlarged tendons; Group B included cases with tendons of normal size but with prominent edema in the peritendinous tissue; and Group C (control group) included cases with no abnormal USD findings. The incidence of subsequent severe tendon injury was significantly higher in the horses in Groups A (25.7%, 28/101) and B (28.3%, 65/212) than in those in Group C (4.9%, 2/41). There were no significant differences in the median period and the median number of races from the first examination to the subsequent tendon injury between Groups A (140 days, 1 race) and B (120 days, 1 race). The results of this study revealed that horses with increased CSA and peritendinous edema are likely to suffer a subsequent severe tendon injury. Also, these two USD findings, i.e., increased CSA and peritendinous edema, indicate the risk of onset of severe SDFT injury.     

Aurasperone A Inhibits SARS CoV-2 In Vitro: An Integrated In Vitro and In Silico Study

Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC₅₀ = 12.25 µM) with comparable activity with the positive control remdesivir (IC₅₀ = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC₅₀ = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC₅₀ = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested M^pro to be its primary viral protein target. More potent anti-SARS CoV-2 M^pro inhibitors can be developed according to our findings presented in the present investigation.     

基于注意力机制神经网络的荒漠区蒸散量模拟

该研究对基于注意力机制的长短期记忆（Attention-Based Long Short Term Memory，AT-LSTM）模型对蒸散量（Evapotranspiration，ET）模拟的可行性和有效性进行验证，以提高环境数据缺失情况下的蒸散量模拟精度。基于盐池县2012-2017年的每30 min环境数据，利用不同环境因子组合构建基于注意力机制的LSTM模型，并将其与极限学习机（Extreme Learning Machine，ELM）模型、支持向量机（Support Vector Machine，SVM）模型、长短期记忆（Long Short Term Memory，LSTM）模型在日尺度、月尺度和季节尺度上进行对比分析。结果表明：与其他3种模型相比，当输入环境因子变化时，AT-LSTM模型模拟精度变化很小，模拟效果均较好。当输入空气温度、净辐射、相对湿度、土壤温度、土壤含水率所有环境因子时，基于AT-LSTM模型的模拟效果最好，均方根误差（Root Mean Square Error，RMSE）为0.013 mm/30 min，平均绝对误差（Mean Absolute Error，MAE）为0.006 mm/30 min，相关系数（Correlation Coefficient，R）值为0.905。且无论是从小时尺度、日尺度和月尺度来看，AT-LSTM模型的模拟效果均优于其他3种模型。在环境因子缺失的情况下，净辐射对盐池县ET的模拟贡献程度最大，仅输入净辐射时，AT-LSTM模型模拟得到的RMSE和MAE分别为0.014、0.007 mm/30 min，R为0.892，模型模拟精度较高，AT-LSTM模型模拟精度高，模型稳定性强，对蒸散量模拟预测具有一定的适用性，仅输入净辐射的AT-LSTM模型可以作为环境数据缺失条件下的蒸散量预测模型。     

施氮量对不同水稻品种氮肥利用率与农艺性状的影响

为探明施氮量对不同水稻品种产量、氮肥利用率和农艺性状的影响,以适合江苏省种植的12个中粳稻（含籼/粳杂交稻）品种为材料,大田种植,设置0N（全生育期不施氮）、200N（全生育期施纯氮200kg/hm ²）和360N（全生育期施纯氮360kg/hm²）3个施氮量处理,研究各品种产量、氮肥利用率和一些农艺性状的差异。结果表明：甬优2640在3个施氮水平产量均最高,库容量大是其高产的主要原因。其他品种产量对氮肥响应的表现不一。在较低氮（0N、200N）条件下,淮稻13号和武运粳30的产量较高,宁粳1号和扬粳4038的产量较低。在高氮（360N）条件下,宁粳1号和扬粳4038产量较高。在较低施氮量条件下,产量和氮肥利用率较高的品种具有较高的干物质积累量、茎蘖成穗率、叶面积指数、粒叶比及群体生长速率,这些指标可作为筛选氮高效品种的农艺性状指标。     

沙枣树胶对红葡萄树提取液抗氧活性保护作用的研究

该文就沙枣树胶对红葡萄树提取液抗氧性的保护作用及其变化进行了初步研究,结果表明红葡萄树提取液具有极强的抗羟自由基(H0·)能力,而抗超氧阴离子自由基(O-2·)的能力较弱,即在空气中易氧化、抗氧活性迅速降低、消失;当加入沙枣树胶初期,其抗氧性能迅速降低,3d后开始上升,15d后其对超氧阴离子自由基清除率达到30%,60d后对羟自由基清除率达到68%,其后,一直保持稳定.在沙枣树胶的多糖活性成分的作用下,红葡萄树胶的抗氧活性显著增强,稳定性提高.研究结果初步表明,沙枣树胶对红葡萄树提取液的抗氧活性具有显著的保护作用.