全文获取类型
收费全文 | 1793篇 |
免费 | 119篇 |
国内免费 | 131篇 |
专业分类
林业 | 152篇 |
农学 | 51篇 |
基础科学 | 111篇 |
173篇 | |
综合类 | 863篇 |
农作物 | 99篇 |
水产渔业 | 78篇 |
畜牧兽医 | 294篇 |
园艺 | 162篇 |
植物保护 | 60篇 |
出版年
2024年 | 13篇 |
2023年 | 20篇 |
2022年 | 63篇 |
2021年 | 87篇 |
2020年 | 67篇 |
2019年 | 66篇 |
2018年 | 60篇 |
2017年 | 58篇 |
2016年 | 43篇 |
2015年 | 88篇 |
2014年 | 74篇 |
2013年 | 87篇 |
2012年 | 145篇 |
2011年 | 121篇 |
2010年 | 145篇 |
2009年 | 138篇 |
2008年 | 154篇 |
2007年 | 112篇 |
2006年 | 116篇 |
2005年 | 101篇 |
2004年 | 75篇 |
2003年 | 33篇 |
2002年 | 45篇 |
2001年 | 60篇 |
2000年 | 40篇 |
1999年 | 16篇 |
1998年 | 2篇 |
1995年 | 2篇 |
1994年 | 5篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1963年 | 1篇 |
1961年 | 1篇 |
1957年 | 1篇 |
1930年 | 1篇 |
排序方式: 共有2043条查询结果,搜索用时 78 毫秒
1.
2.
通过对棉花不同时期去主茎叶、叶枝叶、果枝叶和全部叶片的研究,初步探讨了棉花叶源对库的影响.结果表明:无论蕾期还是盛铃期,叶片是棉花光合产物的主要来源,没有叶片棉花就不能正常生长,也很难保证其产量;不同部位的叶片中,主茎叶的作用显著,蕾期去主茎叶后株高日增长量减少0.5 cm以上,干物质积累明显下降,盛铃期则导致棉铃大量脱落,成铃平均减少2个以上;去果枝叶的作用相对较小,去叶枝叶几乎无影响. 相似文献
3.
真核表达载体构建表达PRV闽A株gE基因表位抗原编码区片段的重组质粒 总被引:2,自引:1,他引:1
根据伪狂犬病病毒闽A株gE基因表位抗原编码区的序列与身份种真核表达载体pPICZaA、pAcGP67A序列与特性分别设计了两对PCR引物。通过PCR方法扩增到了两端具有不同酶切位点的gE基因表位抗原编码片段。将这2个片段分别克隆到pPICZaA与pAcGP67A载体,转化大肠杆菌TOP10菌档及XL1-Blue菌株,获得了含伪狂犬病病毒闽A株gE基因表位抗原编码区的重组质粒pICZaA-FS与pAcGP67A-FS。序测定结果显示两个重组质粒中插入片段的大小与方向均正确。 相似文献
4.
伪狂犬病病毒(PRV)是α疱疹病毒属的一个成员,为伪狂犬病的病原,猪是伪狂犬病病毒的自然宿主,该病原一旦感染猪,则产生潜伏感染,其致死率随猪年龄的增加而降低。由于伪狂犬病的暴发引起畜牧业的巨大经济损失,许多国家都进行免疫接种预防该病,而该病在不同的国家地区危害不同,所造成的经济损失也不一样,另外各国的经济发展不平衡,因此对该病所采取的防制策略亦有所不同,文章综述了美国,日本,欧共体及其成员国之间对伪狂犬病采取的防制策略及取得的进展,并根据我国伪狂犬病的流行现状,提出了应采取的控制策略。 相似文献
5.
利用芥蓝×青花菜DH群体构建AFLP连锁图谱 总被引:9,自引:2,他引:9
利用青花菜(Brassica oleracea var. italica) 与芥蓝(Brassica oleracea var. alboglabra) 杂交后代双单倍体(DH) 群体为材料, 通过AFLP技术构建了一个甘蓝类作物较高密度的遗传连锁图谱。该图谱包括9个主要连锁群及2个小连锁群, 总图距801.5 cM, 含337个AFLP标记, 标记间平均距离为3.6 cM,为甘蓝类作物基因定位、比较基因组学及重要经济性状QTL分析提供了一个框架图谱。 相似文献
6.
7.
AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI. 相似文献
8.
AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of [3H]-TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited ratthymus lymphocytes proliferation[(0.14±0.03)A vs(0.32±0.16)A,P<0.05],but this ef ect had no relationship with the concentration of c-myc antisense oligonucleot ide.c-myc antisense oligonucleotide decreased the expression of c-myc mRNA in rat thymus lymphocytes. CONCLUSION: c-myc antisense oligonucleotide inhibited rat thymus lymphocyte proliferation. 相似文献
9.
AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg-1·d-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue. 相似文献
10.
AIM: To investigate effect of atrial natriuretic peptide (ANP) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rat. METHODS: Mean arterial blood pressure (MAP) was recorded with model 6280 physiology intelligentialize grapher, nitric oxide (NO) and endothelin (ET) concentrations in plasma were measured after lipopolysaccharide (LPS) or following LPS ,ANP was injected into vein in rats. After experiment,lung water as well as pulmonary histopathological changes was measured and observed, respectively. RESULTS: Administration of LPS elicited a persistence decrease in MAP (8.1 kPa±2.6 kPa,at 4 h,P<0.01 vs control); NO and ET concentration in plasma was evident higher than that in control group, respectively (P<0.01); Wet-dry ratio of lung was higher than that in control group (5.15±0.43,at 4 h) (P<0.05); Alveolus detelectasis was observed and pulmonary mesenchyme was thicker than that in control group. No erythrocyte and leukocytes in alveolus,which show an interstitial pulmonary edema, was observed in LPS+ANP group, ANP maintained MAP at higher levels (13.35 kPa±2.93 kPa, at 4 h, P<0.05 vs LPS) after an transient decline when LPS was injected; NO and ET concentration of plasma had all significantly decrease, respectively (P<0.05 vs LPS, at 4 h); Wet-dry ratio of lung was lower than LPS group (4.57±0.35, P<0.05). Compared with control group the ratio was not evident difference (P>0.05); The histopathological of lung displayed markedly improved. CONCLUSION: ANP attenuates ALI induced by LPS in the rat. The effect of ANP may be via decreasing secretion of ET,NO and regulation arterial blood pressure. 相似文献