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Morio Imada Takashi Kunisaki Nobuya Mizoue Yukio Teraoka 《Journal of Forest Research》1997,2(2):89-93
A spacing experiment for Japanese oak (Quercus mongolica var.grosseserrata) with Nelder's design have been studied at the Kyushu University Forest in Hokkaido, since 1975. The oak seedlings were planted
according to systematic spacings which were 20 levels varying gradually from 40,816 to 1,086 seedlings per hectare with concentric
circular arcs (22 in number) and spokes (41 in number). In 1992 when the planted oak seedlings were 18-year-old, DBH, crown
diameter, total height and clear length of the survival trees were measured. Based on the results obtained from analyzing
the relationships between planting density and the mean values of each block, the optimum planting density should be 7,241
seedlings per hectare.
The title is tentative translation from the original Japanese title by the authors of this paper. 相似文献
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Saito M Saito K Kunisaki N Kimura S 《Journal of agricultural and food chemistry》2002,50(24):7169-7174
We have investigated the inhibitory effects of polyphenols from natural products, such as green tea, bilberry, grape, ginkgo, and apple, on rainbow trout gelatinase activities. Gelatinases from the skin, muscle, and blood of rainbow trout contained serine proteinase, metalloproteinase, and other proteinase activities as measured by gelatin zymography. The polyphenols of green tea caused the strong inhibition of some gelatinase activities when compared with those of the other products. This inhibition was quite similar to that of metalloproteinase by ethylenediaminetetraacetic acid, suggesting that the effects of green tea polyphenols on proteinase activities are specific for metalloproteinases. The major catechins of green tea polyphenols were then separated and identified by reverse-phase chromatography to be (-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epicatechin. The effects of these catechins on gelatinase activities were examined; the most potent inhibitor of metalloproteinase activities was found to be EGCG. These results have indicated that green tea polyphenols including EGCG are useful for regulating metalloproteinase activities of fish meat. 相似文献
3.
Manabu MATSUDA Keiko KUROSAKI Naomichi OKAMURA 《The Journal of reproduction and development》2014,60(4):274-279
Exposure of mice to a high dose of estrogens including diethylstilbestrol (DES) during the neonatal period modifies the
developmental plan of the genital tract, which leads to various permanent changes in physiology, morphology and gene expression.
These changes include development of an abnormal vaginal epithelium lined with hyperplastic mucinous cells accompanied by
Tff1 gene expression in mice. Here, the influence of vitamin D on the direct effect of estrogen on the
developing mouse vagina was examined. The mid-vagina of neonatal mice was cultured in a serum-free medium containing estradiol-17β
(E2) and various concentrations of 1,25-dihydroxyvitamin D3 (1,25(OH)2D) ex
vivo and then was transplanted under the renal capsule of ovariectomized host mice for 35 days. Exposure to
E2 alone caused the vaginal tissue to develop estrogen-independent epithelial hyperplasia and to express TFF1 mRNA,
while addition of a low nanomolar amount of 1,25(OH)2D added at the same time as E2 to the culture medium
attenuated the effects of estrogen. Expression of vitamin D receptor was also evident in the neonatal mouse vagina. Interestingly,
addition of 25-hydroxyvitamin D3, a pro-activated form of vitamin D, at the micromolar level was found to be potent in
disrupting the developmental effects of E2, while cholecalciferol was not at least at the dose examined.
Correspondingly, expression of Cyp27B1, a kidney-specific 25-hydroxyvitamin D hydroxylase, was evident in the neonatal mouse
vagina when examined by RT-PCR. In addition, simultaneous administration of 1,25(OH)2D successfully attenuated
DES-induced ovary-independent hyperplasia in the vagina in neonatal mice in vivo. Thus, manipulation of vitamin D
influenced the harmful effects of estrogens on mouse vaginal development. 相似文献
4.
Priyanka KUMARI Kyung Yeon EO Woo-Shin LEE Junpei KIMURA Naomichi YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):850
Leptospira, Giardia intestinalis and Toxoplasma gondii infections are reported in humans and animals worldwide, but molecular surveillance of these pathogens in Korean wildlife is still limited. Here, we examined the prevalence of these pathogens in environmental feces of Eurasian otters, leopard cats and raccoon dogs using nested PCR followed by DNA sequencing. G. intestinalis was detected in all of three animals, while T. gondii was detected only in leopard cats. Leptospira wolffii was detected in raccoon dog and Eurasian otter. Our results suggest that these animals can act as a reservoir of these zoonotic pathogens. Consistent monitoring of these pathogens in wildlife is needed to prevent from their infections in humans and livestock in Korea. 相似文献
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Hisashi Sugita Takashi Kunisaki Takeyasu Takahashi Ryoichi Takahashi 《Journal of Forest Research》2008,13(3):155-164
Naturally regenerated trees in young Cryptomeria japonica plantations were investigated in 141 quadrats of 10 × 50 m within a watershed of 1,000 ha, and factors affecting their composition
and abundance were examined. The species composition of naturally regenerated trees was classified into four types. Dominant
species were Swida controversa, Magnolia hypoleuca and Pterocarya rhoifolia in Type A1, Betula maximowicziana, M. hypoleuca, Quercus crispula and Castanea crenata in Type A2, Q. serrata and C. crenata in Type B1, and Pinus densiflora in Type B2. The results of path analysis showed significant influences of previous forest type and geology among the factors
that correlated with the species composition of naturally regenerated trees; previous forest type in particular showed a higher
absolute path coefficient value. Species composition types of naturally regenerated trees corresponded to the previous forest
types: Types (A1 and A2) corresponded to the natural forests composed of Thujopsis dolabrata var. hondai, Fagus crenata, Aesculus turbinata, etc., Type B1 corresponded to the secondary Q. crispula and Q. serrata forest, and Type B2 corresponded to the secondary P. densiflora forest, respectively. The abundance of naturally regenerated trees was strongly affected by geology; i.e., plantations on
soft-sedimentary dacitic tuff contained great amounts of colonizing P. densiflora trees. 相似文献
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SUMMARY: In a study of the relationship between structural changes and rheological parameters of raw and cooked abalone meat, abalone Haliotis discus was cooked in boiling water for 3 h, then cut up and separated into cross- and vertical sections. Structural change was observed using a light microscope and scanning electron micrography. The rheological parameters were obtained by stress-relaxation experiments. Microscopic photography revealed that structural change between muscle fibers in both sections of the cooked meat was greater than that in the raw meat. The elastic modulus and rupture strength in both sections of cooked meat showed no difference between the cross- and vertical sections, and were smaller than those for the raw meat. The relaxation time for the cooked meat was also greater than for the raw meat. These results were mainly because of collagen being gelatinized during heating. These changes were also observed by staining collagen and differential scanning calorimetry. 相似文献
8.
Localization of a novel RNA-binding protein, SKIV2L2, to the nucleus in the round spermatids of mice
Osman BA Kawashima A Tamba M Satoh E Kato Y Iki A Konishi K Matsuda M Okamura N 《The Journal of reproduction and development》2011,57(4):457-467
In our previous study (Kawashima et al., Biol Reprod 2009; 80: 1293-1304), we suggested that the first cycle of spermatogenesis recovered from busulfan-induced temporary arrest was a good model system to analyze the proteins expressed at the specific stages of spermatogenesis in the mouse, and this has been confirmed in the present paper. Namely, six-week-old mice were injected with busulfan at 20 mg/kg body weight. The germ cells except for the undifferentiated spermatogonia disappeared by 32 days after injection. The surviving spermatogonia started to proliferate, and spermatogenesis was entirely recovered about 77 days after injection. By proteome analysis of the busulfan-treated testis during the process of recovery of spermatogenesis, we identified a protein that was expected to be expressed in the spermatogenic cells as Superkiller viralicidic activity-2-like-2 (SKIV2L2). Skiv2l2 mRNA was found in the kidney, epididymis and heart as well as the testis. In the testis, Skiv2l2 mRNA was shown to be highly expressed in the spermatocytes at stages I to VI. On the other hand, SKIV2L2 protein was found to be predominantly localized in the nuclei of round spermatids. In accordance with the fact that SKIV2L2 belongs to the Ski2 family within the superfamily 2 of RNA helicases, it has been shown that SKIV2L2 has both the RNA-binding and ATPase activities. 相似文献
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Itami T Tamaru N Kawase K Ishizuka T Tamura J Miyoshi K Umar MA Inoue H Yamashita K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1603-1609
Cardiovascular effects of tramadol were evaluated in dogs anesthetized with sevoflurane. Six beagle dogs were anesthetized twice at 7 days interval. The minimum alveolar concentration (MAC) of sevoflurane was earlier determined in each dog. The dogs were then anesthetized with sevoflurane at 1.3 times of predetermined individual MAC and cardiovascular parameters were evaluated before (baseline) and after an intravenous injection of tramadol (4 mg/kg). The administration of tramadol produced a transient and mild increase in arterial blood pressure (ABP) (P=0.004) with prolonged increase in systemic vascular resistance (SVR) (P<0.0001). Compared with baseline value, mean ABP increased significantly at 5 min (119% of baseline value, P=0.003), 10 min (113%, P=0.027), and 15 min (111%, P=0.022). SVR also increased significantly at 5 min (128%, P<0.0001), 10 min (121%, P=0.026), 30 min (114%, P=0.025), 45 min (113%, P=0.025) and 60 min (112%, P=0.048). Plasma concentrations of tramadol were weakly correlated with the percentage changes in mean ABP (r=0.642, P<0.0001) and SVR (r=0.646, P<0.0001). There was no significant change in heart rate, cardiac output, cardiac index, stroke volume, pulmonary arterial pressure, right atrial pressure and pulmonary capillary wedge pressure. In conclusion, the administration of tramadol produces a prolonged peripheral vascular constriction in dogs anesthetized with sevoflurane, which is accompanied with a transient and mild increase in arterial blood pressure. It also indicated that the degree of vasoconstriction might depend on the plasma concentration of tramadol. 相似文献
10.
Rana Kobayashi Kentaro Nagaoka Naomichi Nishimura Satoshi Koike Eiki Takahashi Kimie Niimi Harutaka Murase Teruo Kinjo Takamitsu Tsukahara Ryo Inoue 《Animal Science Journal》2020,91(1)
Fecal microbiota in seven different monogastric animal species, elephant, horse, human, marmoset, mouse, pig and, rat were compared using the same analytical protocol of 16S rRNA metagenome. Fecal microbiota in herbivores showed higher alpha diversity than omnivores except for pigs. Additionally, principal coordinate analysis based on weighted UniFrac distance demonstrated that herbivores and pigs clustered together, whereas other animal species were separately aggregated. In view of butyrate‐ and lactate‐producing bacteria, predominant genera were different depending on animal species. For example, the abundance of Faecalibacterium, a known butyrate producer, was 8.02% ± 3.22% in human while it was less than 1% in other animal species. Additionally, Bifidobacterium was a predominant lactate producer in human and marmoset, while it was rarely detected in other omnivores. The abundance of lactate‐producing bacteria in herbivores was notably lower than omnivores. On the other hand, herbivores as well as pig possess Fibrobacter, a cellulolytic bacterium. This study demonstrated that fecal microbiota in herbivorous animals is similar, sharing some common features such as higher alpha diversity and higher abundance of cellulolytic bacterium. On the other hand, omnivorous animals seem to possess unique fecal microbiota. It is of interest that pigs, although omnivore, have fecal microbiota showing some common features with herbivores. 相似文献
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