Abundance of Iron-Oxidizing Thiobacilli and Biological Sulfur Oxidation Potential from Soil Impacted by Coal and Coal Refuse Piles

This study was conducted to assess the abundance of iron-oxidizing bacteria and biological sulfur oxidation potential from soil impacted by coal and coal refuse from two coal-burning electric power facilities located at the U.S. Department of Energy's Savannah River Site (Aiken, S.C.) and the South Carolina Electric and Gas Site at Beech Island, S. C. Significantly higher MPN counts of iron-oxidizing bacteria were obtained from samples collected at the confluence of a coal storage runoff containment basin, a coal reject (refuse) pile, and an adjacent wetland at the Savannah River Site. Significant differences in pH, sulfate-S, ferrous- and ferric-iron were also obtained between sampling locations. No significant differences in ferric/ferrous ratios were determined. These ratios however, exceeded a value of 2.0 when sample pH values were less than 4.5. Under optimal conditions, biological thiosulfate-S oxidation potentials (in vitro) showed a 4- to 7-day lag in the appearance of sulfate-S, and a final pH (after twenty-four days of perfusion) of 1.97 to 3.90. These results indicate that contamination of subsurface water by acidic leachate derived from thionic bacterial activity will occur if coal and coal refuse piles are not confined by an impermeable surface or containment facility.     

Heterotrophic N2-fixation in arid soil crusts

The cryptogamic soil crusts of the Great Basin Artemisia, Ceratoides, and Atriplex plant communities contain a significant heterotrophic N₂-fixing microbial population in addition to the predominating filamentous cyanobacteria. The bacterial association with the cyanobacteria exhibits a phycosphere-like effect. Heterotrophically fixed N gains reached 17.5 μg N· g^?1 of soil (23.1% increase above the initial soil N content) and 45.9 μg N·g^?1 of soil (57.4% increase) after 3 and 5 weeks, respectively. (NH₄)₂SO₄ and native plant material amendments to soil resulted in a 41–100% reduction in N₂-fixation. The potential input of N to soil crusts may be reduced in the presence of shrub-produced allelochemic agents and by concurrent denitrification.     

Soil biological properties of a montane forest sere: Corroboration of Odum's postulates

Changes in organic C content, N component pools, Shannon-Weaver diversity index (H′) of microbial populations, nitrification potential, and ATP and dehydrogenase activities were examined in soils along a montane meadow-aspen-fir-spruce sere.Along the sere organic C increased from 2.15 to 26.8%, total N from 0.13 to 0.98%, C: N ratios from 17 to 27, total NH₄⁺ from 103 to 850 μg g^?1, total NH₄⁺:NO₃^? ratios from 69 to 326, and microbial diversity index, H′, from 0.87 to 1.28. Coefficients of determination, r², for H′ vs organic C and total N in A-horizons, were 0.99 and 0.98, respectively, and H′ vs combined O- and A-horizons 0.68 and 0.70, respectively, indicating the presence of different microbial communities in the mineral and forest floor soils. Radiocarbon dating of humic acids and humin showed the longest mean residence times (920 and 1050 yr BP) in the meadow soils, suggesting a more efficient organic matter turnover and selective accumulation of recalcitrant organic components than in soils of more mature stages. The ATP content and dehydrogenase activity values were not statistically different in the forest sequence soils. Rates of nitrification potentials measured in vitro increased along the sere in the surface soils.Information obtained from seral soil variables supported hypothesized successional trends relating to organic matter content, species diversity, nutrient cycling, nutrient exchange rate and nutrient conservation. Nitrification potentials of soils, however, contradicted the postulate that nutrient conservation increases as an ecosystem matures.